Relations Between Changes in Contents of Phenolic Acids and Phenolic Polymers and Induced Resistance to Bacterial Blight in Rice under Nickel Treatment

doi:10.3969/j.issn.1001-7216.2010.04.018

Abstract

Abstract: Changes in accumulation of total phenolics, phenolic acids, phenolic polymers and activity of cell wall bound peroxidase (POD) were investigated in leaves of rice exposed to nickel treatment. Ten soluble phenolic acids and seven bound phenolic acids were detected. The levels of soluble chlorogenic acid and salicylic acid were significantly promoted by nickel treatment, whereas the contents of their bound forms remained unchanged. Nickel treatment evidently increased the concentrations of both soluble and bound ferulic acid. However, nickel treatment led to a significant decrease in the contents of soluble syringic acid and pcoumaric acid but an increase in those of their bound forms. Moreover, the pronounced accumulation of phenolic polymers and enhancement in activities of cell wall bound POD, either with ferulic acid or syringaldazine as its substrate, were registered in rice leaves following nickel treatment. The present results combined with the previous study suggest that the accumulation of phenolic acids and phenolic polymers are involved in the establishment of nickelinduced resistance against bacterial blight in rice.

Key words: phenolic acids, phenolic polymers, induced resistance, bacterial blight, nickel, rice (Oryza sativa)

摘要： 研究了镍处理下水稻叶片中总酚、酚酸、酚多聚物积累和细胞壁结合态过氧化物酶（POD）活性的变化。镍处理导致总酚含量显著上升，检测到10种可溶性酚酸和7种结合态酚酸。镍处理下可溶性绿原酸和水杨酸含量明显上升，而它们的结合态含量变化不明显；镍处理显著提高可溶性和结合态阿魏酸的含量，显著降低可溶性丁香酸和对香豆酸的含量，却诱导其结合态含量增加。镍处理后酚多聚物明显积累，以阿魏酸和丁香醛为底物的细胞壁结合态POD的活性显著升高。这些结果结合以前的研究提示，酚酸和酚多聚物积累参与了镍诱导的水稻对白叶枯病的抗性。

关键词: 酚酸, 酚多聚物, 诱导抗性, 白叶枯病, 镍, 水稻

TAN Xin-zhong,PENG Xi-xu,HU Yao-jun,TANG Xin-ke,ZHOU Ping-lan,DENG Xiao-bo,WANG Hai-hua*. Relations Between Changes in Contents of Phenolic Acids and Phenolic Polymers and Induced Resistance to Bacterial Blight in Rice under Nickel Treatment[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2010.04.018 .

谭新中,彭喜旭,胡耀军,唐新科,周平兰,邓小波,王海华. 镍处理下水稻酚酸和酚多聚物含量的变化与白叶枯病诱导抗性的关系[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2010.04.018 .

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