Genetic Analysis and Molecular Mapping of a PhotoperiodThermoSensitive Genic Male Sterile Gene (PTGMS2-1) in Rice Line Guangzhan 63S

doi:10.3969/j.issn.1001-7216.2010.04.016

Abstract

Abstract: Guangzhan 63S, a photoperiodthermosensitive genic male sterile (PTGMS) line, was one of the most widely used PTGMS lines in twoline indica hybrid rice breeding system in China. Genetic analysis indicated that the sterility of Guangzhan 63S was controlled by a single recessive gene using the F2 population derived from a cross between the Guangzhan 63S and Han 1587. Molecular mapping was performed on the F2 mapping population using SSR and InDel markers and bulkedsegregant analysis(BSA). The PTGMS21 was restricted to a 390kb region on chromosome 2 between the markers S24 and S227, at genetic distances of 0.5 cM and 1.1 cM, respectively, and cosegregated with S224.

Key words: photoperiodthermosensitive genic male sterility, genetic analysis, molecular mapping

摘要： 水稻光温敏雄性核不育系广占63S的不育基因来源于光敏核不育系农垦58S，其育性转换表现为温敏型，是迄今两系杂交水稻中应用最广泛的籼型光温敏核不育系之一。对广占63S/旱1587组合的F₂分离群体进行不育基因的遗传分析，结果表明广占63S的不育性受1对隐性不育基因控制。利用SSR和InDel分子标记技术，结合BSA法，以广占63S与旱1587组合的F2分离群体为材料，将不育基因（暂命名为PTGMS2-1）定位于第2染色体标记S2-4与S2-27之间物理距离390 kb的区间内，与两标记间的遗传距离分别为0.5和1.1 cM，与标记S2-24共分离。

关键词: 光温敏雄性不育性, 遗传分析, 分子定位

WANG Bao-he,XU Jian-jun,WU Yin-hui,ZHU Jin-yan,LI Sheng-qiang,ZHOU Yong,CHENG Xiao-tao,LIANG Guo-hua. Genetic Analysis and Molecular Mapping of a PhotoperiodThermoSensitive Genic Male Sterile Gene (PTGMS2-1) in Rice Line Guangzhan 63S[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2010.04.016 .

王宝和,徐建军,吴银慧,朱金燕,李生强, 周勇, 程小涛, 梁国华,. 水稻光温敏雄性核不育系广占63S不育基因PTGMS2-1的遗传分析与分子定位[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2010.04.016 .

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