Genetic Diversity and Pathogenicity Variation of Different Rhizoctonia solani Isolates in Rice from Zhejiang Province, China

doi:DOI: 10.3969/j.issn.1001-7216.2010.12

Abstract

Abstract: Sixty Rhizoctonia solani strains were isolated from rice sheath blight samples collected from ricegrowing regions of Zhejiang Province, China. Hyphal fusion identification revealed that all the 60 strains belonged to anastomosis group AG1, as they all fused with the three intraspecific group of AG1 group(AG1ⅠA, AG1ⅠB and AG1ⅠC). Fiftyeight strains out of the 60 strains fused with the standard strain of AGBⅠ group. Pathogenicity identification analysis in vitro indicated significant difference in pathogenicity among the strains. TTZF1, the most pathogenetic strain, was significantly more pathogenetic than the other 52 strains. Further internal transcribed spacer(ITS) analysis indicated that phylogenetic tree coincided with the result of hyphal fusion identification, and was irrelevant with pathogenicity difference.

Key words: rice sheath blight, Rhizoctonia solani, anastomosis group, internal transcribed spacer, phylogenesis, pathogenicity

摘要： 鉴定了从浙江省各地采集的60株水稻纹枯病菌株的菌丝融合群归属情况。该60个菌株均与AG1融合群的3个亚群AG1ⅠA、AG1ⅠB、AG1ⅠC的标准菌株融合，当属AG1融合群。其中，有58株均与标准菌株AGBⅠ融合，所有菌株与其他标准菌株均无融合现象。通过离体接种对菌株致病力进行鉴定，发现各菌株间致病力差异显著。致病力最强的TTZF1与其中52个菌株差异极为显著。进一步的转录间隔区（ITS）序列分析显示，系统发育树的结果与融合群鉴定的结果一致，与菌株间致病力差异无相关性。

关键词: 水稻纹枯病, 立枯丝核菌, 菌丝融合群, 转录间隔区, 系统发育, 致病力

CHEN Tao, ZHANG Zhen, CHAI Rong-yao,WANG Jiao-yu,MAO Xue-qin,QIU Hai-ping,DU Xin-fa,JIANG Hua, WANG Li-an,WANG Yan-li .

Genetic Diversity and Pathogenicity Variation of Different Rhizoctonia solani Isolates in Rice from Zhejiang Province, China

[J]. Chinese Journal of Rice Science, DOI: DOI: 10.3969/j.issn.1001-7216.2010.12 .

陈涛,张震,柴荣耀,王教瑜,毛雪琴,邱海萍,杜新法,姜华,王立安. 浙江省水稻纹枯病菌的遗传分化与致病力研究[J]. 中国水稻科学, DOI: DOI: 10.3969/j.issn.1001-7216.2010.12 .

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