Abstract:

The near isogenic lines(NILs)of a rice few-tillering control gene ft1,based on the genetic background of 02428,were established by crossing G069 with 02428 and continuous backcrossing by using 02428 as the backcrossing parent and named 02428-ft1.Cytological study indicated that the development of lateral meristem was inhibited by the few-tillering control gene.Genome-wide expression analysis between 02428-ft1 and 02428 was carried out by using an Affymetrix gene chip including 59 712 cloned or predicted gene feature cDNA sequences.It was found that there were 136 cDNA showing significant change in its expression level,including 27 activated and 30 silenced genes,which were significantly controlled by the few-tillering gene in the NILs 02428-ft1.Function analysis of the differently expressed genes by using the on-line analysis systems of Affymetrix,NCBI and TIGR showed that the functions of the differently expressed genes were extensive,including transcription factors,kinases,function proteins,regulatory proteins and so on.

Key words: fewtillering, rice, near isogenic line, gene chip, genomewide expression analysis, function annotation

摘要： 利用水稻寡分蘖突变体G069与广亲和材料02428杂交和连续回交，建立了寡分蘖基因在02428基因组背景下的近等基因系02428ft1.在分蘖始期对其分蘖节位的石蜡切片观察表明，寡分蘖基因抑制了水稻侧芽的发生。利用含有59 712个预测基因或已知基因的表达特征序列的基因芯片进行全基因组表达谱分析，结果表明共有136个cDNA克隆的表达在近等基因系02428ft1中发生了变化，其中27个被激活，30个沉默，受到了寡分蘖基因的明显调控。功能分析表明, 这些受寡分蘖基因调控的基因功能分布较为广泛，包括转录因子、激酶、功能蛋白和调节蛋白等，为水稻分蘖调控机制的研究提供了相关信息。

关键词: 寡分蘖, 水稻, 近等基因系, 基因芯片, 全基因组表达分析, 功能注释