Chinese Journal OF Rice Science

Chinese Journal of Rice Science

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Establishment of Sequence Related Amplification Polymorphism and Segregation of the Polymorphic Loci in F2 Population for Rice

JIANG Shu-kun , ZHONG Ming , XU Zheng-jin , ZHANG Li , ZHANG Xi-juan , MA Hui , CHEN Li-jing , GUO Zhi-fu   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10

水稻相关序列扩增多态性反应体系的建立及其多态性位点在F2群体中的分离分析

姜树坤1;钟鸣1，*;徐正进2 ;张丽1 ;张喜娟2 ;马慧1;陈丽静1;郭志富1   

  1. 1沈阳农业大学 辽宁省农业生物技术重点实验室， 辽宁 沈阳 110161； 2沈阳农业大学 辽宁省北方粳稻育种重点实验室， 辽宁 沈阳 110161； *通讯联系人， E-mail: Zhming@syau.edu.cn

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Abstract: An effective PCR protocol for detecting sequence related amplified polymorphism(SRAP) in rice was developed.SRAP markers were used for segregation analysis in an F2 population derived from a cross between Shennong 606 and Lijiangxintuanheigu.Among 110 primer pairs,35 pairs generated 143 polymorphic bands with an average of 4.09 polymorphic bands per primer pair and 24 markers(16.78%) showed the genetic distortion(P<0.05).Of them,11 markers deviated towards pollen parent Shennong 606 and 12 markers towards ovule parent Lijiangxintuanheigu,only one towards heterozygote.It was found that the segregation distortion might be caused by gamete and zygote jointly.

Key words: rice, sequence related amplification polymorphism(SRAP), amplified protocol, segregation distortion

摘要： 建立了一套完整、稳定、高效的水稻相关序列扩增多态性(SRAP)反应体系，同时利用110对SRAP引物对水稻品种沈农606和丽江新团黑谷进行比较扩增，其中35对引物可以检测到多态性，约占所用引物的31.82%。用该35对引物进一步对上述两个品种配制的杂交后代的F2进行检测，共扩增出1683条带，平均每对引物48条带，其中多态性条带143条，平均每对引物4.09条多态性条带。有24个多态性位点在F2群体中的分离显著或极显著地偏离了理论比值而表现异常分离，比率为16.78%。这些偏分离位点中偏向沈农606基因型的有11个;偏向丽江新团黑谷基因型的有12个;仅有1个偏杂合体类型。卡方检验说明，标记位点的偏分离是配子体和合子体选择共同作用的结果。

关键词: 水稻, 相关序列扩增多态性