Abstract: In order to improve the precision of marker-assisted selection for fragrance gene in rice, a functional marker GRFM04 has been developed based on the deletion of 8 bp in DNA sequence of BAD2 gene, namely the fragrance gene, in the fragrant rice varieties. The F2 segregation population derived from a cross of fragrant rice variety Yuefeng B and non-fragrant rice variety Zhenfeng B, and 16 fragrant as well as 6 non-fragrant varieties were analyzed by using the marker GRFM04. The results showed that, the homozygous genotype with fragrance, the heterozygous genotype. and the homozygous non-fragrant genotype could be clearly distinguished and the band types were in completely accordance with the phenotypes of fragrance for all the individual plants in the F2 population and the 22 fragrant and non-fragrant rice varieties. The development of the functional marker GRFM04 will be of practical significance in enhancing the efficiency in genetically improving fragrant traits and fragrant rice breeding.

Key words: rice, fragrance gene, functional marker, marker-assisted selection

摘要： 为了提高水稻香味基因的分子标记辅助选择的准确性，根据水稻隐性香型品种与非香型品种的BAD2基因（即香味基因）序列之间存在8 bp碱基缺失，设计出香味基因fgr的InDel功能标记GRFM04。利用该标记对粤丰B（香型）/振丰B（非香型）的F2分离群体和其他16份香稻品种和6份非香稻品种进行检测验证。依据其PCR扩增产物电泳带型，可以准确地区分出香型纯合基因型、非香型纯合基因型和杂合基因型3种带型，且3种带型与其植株或品种相应的香味性状表现完全呈一一对应关系。

关键词: 水稻, 香味基因, 功能标记, 分子标记辅助选择