Abstract: A safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display.The transcript increased 6 h after the rice seedlings were sprayed with the safener solution and 5 days later,the expression could still be detected.The fragment was recycled from the poly-gel and sequenced,and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice data base,and showed 60%-84% homologous to the gene Yippee in several eukaryotes in amino acid sequence.The fragment was extended to a 831 bp cDNA,a primer was designed and RT-PCR analysis supported the induction result.

Key words: rice, chemical induction, mRNA differential display, S-ethyl-N, N-dipropylthiocarbonate, Yippee gene

摘要： 为寻找化学诱导表达基因，利用mRNA差异显示方法，从除草剂安全剂AD67（苯叉酰胺）诱导处理的水稻品种9311的幼苗中筛选分离得到1个诱导表达增强的cDNA片段。该片段在处理后6 h表达明显增强，直到第5天仍然有表达。从polygel上回收片段并测序，核酸同源序列分析显示它与水稻多个EST和cDNA序列一致性达到100%，氨基酸序列分析表明它与水稻、四膜虫、隐球酵母等真核生物的Yippee基因相似性达60%~84%。序列经延伸得到831 bp的cDNA，由此设计引物并进行RTPCR分析，结果说明该基因在除草剂安全剂AD67诱导后表达增强。

关键词: 水稻, 化学诱导, 差异显示, 苯叉酰胺, Yippee基因