Abstract: A primer design method was developed for detection of point mutation in rice.Twelve primer pairs were designed for detection of point mutation in 12 candidate genes.A single band was amplified successfully with each of 10 out of 12 primers(83%)from the genome and point mutations were detected in IR64 mutants as well.This method can be used for primer design for animals and other plant species also.In addition,common questions encountered in primer design were discussed and the corresponding measures were put forward.

Key words: rice, targeting induced local lesion in genome(TILLING), point mutation detection, primer design, polymerase chain reaction

摘要： 介绍了一种用于水稻点突变检测的PCR引物设计方法。利用该方法根据12个候选基因序列设计了12对引物，其中10对引物可以正常工作，能从基因组中扩增出特异性条带，合格率为83%，并且成功地在突变体中检测到点突变。这一方法不仅可以用于水稻，也可以用于其他动植物。同时，对引物设计过程中常见问题进行了讨论并给出了解决办法。

关键词: 水稻, 定向诱导基因组局部突变, 点突变检测, 引物设计, 聚合酶链式反应