Chinese Journal of Rice Science

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Discussion on Photoreceptor for Negative Phototropism in Rice Root

WANG Yue-xia , WANG Zhong , SUO Biao , GU Yun-jie , WANG Hui-hui , ZHANG Wen-hu , KONG Yu   

  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-10 Published:2007-03-10

关于水稻根负向光性光受体的探讨

汪月霞1,2;王忠1,2,*;索标3;顾蕴洁1,2;王慧慧2;张文虎2;孔妤2   

  1. 1扬州大学 江苏省作物遗传生理重点实验室, 江苏 扬州 225009; 2扬州大学 生物科学与技术学院, 江苏 扬州 225009; 3上海交通大学 农业与生物学院, 上海 201101; *通讯联系人, E-mail: wangzhong@yzu.edu.cn

Abstract: To properly explore the photoreceptor for the negative phototropism in rice(Oryza sativa L.)root,lights with different wavelengths were applied to investigate the effect of light quality on phototropic response.The negative phototropic bending could be induced prominently by blue/UV light,while not be induced by red and far-red light.Absorption spectrum of the extracted solution from rice cap had two peaks under 350 nm and 450 nm accordingly,and the molecular weight of the 120 kD protein of the cap under unilateral light was larger than that under the dark.It suggested that the blue light receptor might be the photoreceptor for the negative phototropism in rice root.

Key words: rice, negative phototropism, root, light quality, photoreceptor

摘要: 为了研究水稻根负向光性的光受体,观察了光质对稻根的负向光性效应,用紫外可见分光光度计测定了水稻根冠浸提液的吸收光谱,并对根冠中的蛋白质进行了SDSPAGE电泳分析。蓝紫光能显著诱导稻根负向光性,而红光无效;根冠浸提液的吸收光谱在350 nm和450 nm各有一个吸收峰;根冠中有120 kD的光受体特征的蛋白条带。推测稻根负向光性的光受体可能为蓝光受体。

关键词: 水稻, 负向光性, 根, 光质, 光受体