Abstract: The full-length of intact ZmC₄Ppc gene is 6781 bp, and it’s difficult to obtain clear bands amplified by PCR, which affected the accuracy of marker-assisted selection (MAS) both in rice and maize. Specific fragments presented only in maize rather than in rice were identified and used for primer design using Primer Premier 5.0. A pair of specific primer termed MRpc (forward: 5′ AAGCAGGGAAGCGAGACG 3′, reverse: 5′ GATTGCCGCCAGCAGTAG 3′) was used for selection of transformed rice and ZmC₄Ppc gene could be highly consitutive expressed at every developmental stage in rice. MRpc was used for MAS of progenies carrying ZmC₄Ppc gene in rice and a transformed rice line FPM881 was gained, which was tested for analyzing the genetic background, PEPCase activity, net photosynthetic rate (Pn), general combining ability (GCA) and specific combining ability (SCA). The results indicated that the FPM881 showed an above 95% similar genetic background, a higher expression of PEPCase and an improved net photosynthetic rate than those of control. Analysis of yield components showed that some of the progenies carrying the ZmC₄Ppc gene had better GCA and SCA for grain yield per plant, number of panicles per plant, and 1000-grain weight compared to the control. It suggested that the introduction of maize ZmC₄Ppc gene via MAS with stable expression could increase rice grain yield and would likely provide a pathway for rice improvement.

Key words: ZmC4Ppc gene, MRpc, marker-assisted selection, rice, maize

摘要： ZmC4Ppc基因全长6781 bp，PCR扩增时带型不清晰，重复性差，影响标记辅助选择的准确性。通过序列比对找到了该基因在玉米上存在而在水稻上不存在的特异片段，利用Primer Premier 5.0设计了1对用于水稻标记辅助选择的特异性引物 （前引物为5′ AAGCAGGGAAGCGAGACG 3′，后引物为5′ GATTGCCGCCAGCAGTAG 3′，命名为MRpc）。结果表明，经MRpc筛选的该基因在各生育阶段都能高效表达， 且为组成性表达。对标记辅助选择后代FPM881进行了遗传背景、PEPCase活性、净光合速率、一般配合力(GCA)和特殊配合力(SCA)分析。 FPM881与对照的遗传背景相似度已达95%以上，ZmC4Ppc在MAS的各世代中能够高效表达。GCA和SCA分析结果表明从FPM881中能够筛选出单株产量、有效穗和千粒重优于对照、GCA较高的改良恢复系并得到SCA显著高于对照的杂交组合。试验结果说明，ZmC4Ppc基因高效表达能提高经济产量，在水稻遗传背景中发挥其基因效应。

关键词: ZmC4Ppc基因, 特异性引物, 标记辅助选择