Abstract: An experiment was conducted to analyze the allelic variation of the Wx gene in 50 non-glutinous rice varieties(lines) using molecular markers RM190 [for(CT)n single sequence repeat] and 484/W2R-ACCⅠ(for G/T SNP).A total of seven homozygous(CT)n-type variations,namely(CT)20,(CT)19,(CT)18,(CT)17,(CT)14,(CT)11 and(CT)10,and one heterozygous genotype(CT)11/(CT)18 were detected with RM190,of which(CT)11 and(CT)18 were predominant.Two homozygous(G/G and T/T genotypes) and one heterozygous G/T Wx genotype were detected using 484/W2R-ACCⅠ.Most of the materials with a(CT)11 of RM190 were G/G SNP of 484/W2R-ACCⅠ,while(CT)18 were T/T.The materials tested were grouped into 10 categories using the two markers together.Further analysis indicated that 59.3% variance of amylose content was attributed to the polymorphism of Wx gene resulted from RM190 marker,whereas 56.1% and 24.6% of the variance in amylose content and gel consistency were respectively attributed to the polymorphism of Wx gene resulted from 484/W2RACCⅠmarker.Furthermore,in the presence of SSR and CAPS markers together,72.4% of the variance in amylose content could be explained.The application prospects of the two markers in breeding were also discussed.

Key words: waxy gene, microsatellite, cleaved amplified polymorphic sequence marker, gelatinization temperature, gel consistency, amylose content, rice, polymorphism

摘要： 利用RM190和484/W2RACCⅠ标记分析了50个水稻非糯品种的Wx基因的等位性变异。其中，RM190揭示出7种(CT)n纯合变异类型和1种杂合类型，分别为 (CT)20、(CT)19、(CT)18、(CT)17、(CT)14、(CT)11、(CT)10和(CT)11/(CT)18，并以（CT）11和（CT）18两种类型为主；484/W2RACCⅠ共揭示出2种纯合类型和1 种杂合类型，分别为G/G型、T/T型及G/T型；(CT)11大多为G/G型，(CT)18大多为T/T型，这两个标记可将供试材料划分为10种等位基因变异类型。进一步分析表明，RM190揭示的Wx基因多态性可以解释直链淀粉含量变异的59.3 %；484/W2RACCⅠ揭示的Wx基因多态性可以分别解释直链淀粉含量及胶稠度变异的56.1%和24.6%；而两标记共同可解释直链淀粉含量变异的72.4%。还对这两个标记在育种实践中的应用前景进行了探讨。

关键词: 蜡质基因, 微卫星标记, 酶切扩增多态性序列标记, 糊化温度, 胶稠度, 直链淀粉含量, 水稻, 多态性