Abstract: Two ABA-specifically-inducible proteins from rice callus,were isolated and purified by precipitation with 65%-100% saturated(NH4)2SO4,followed by the DEAE-sepharose,TSK-gel,and two-dimension electrophoresis.Iso-electric point(pI) of the proteins with the same molecular mass(24.5 kD)were 6.1 and 6.9 respectively.Western blotting analysis indicated that the expression of the proteins at the different tissues had obvious diversity.The A1(pI 6.1) protein was(detected) in the rice callus treated with ABA and the seed embryos(SE),but not in young plants,the callus without ABA treatment and the white dry callus(WDC).The A2(pI 6.9) protein was found in the callus treated with ABA,WDC and SE,but not in other materials without ABA treatment.These two proteins might play some important roles in the processes of seed embryo(or somatic embryos) formation.

Key words: rice, callus, abscisic acid, specifically-inducible protein, purification, expression

摘要： 用硫酸铵分级沉淀、离子交换（DEAESepharose和TSKgel）的方法，结合SDSPAGE和2D电泳（twodimension electrophoresis）技术，从水稻愈伤组织中分离并精制了两个分子量相同（24.5 kD）, 等电点分别为61和6.9的脱落酸（ABA）特异诱导蛋白。Westernblot印迹分析发现，这两个蛋白在不同组织中的表达差异明显。A1（pI 6.1）蛋白在ABA处理的水稻愈伤组织和种子胚中表达，但在未经ABA处理的材料(包括幼芽、愈伤组织)和白色干燥性愈伤组织中没有检测到表达；A2（pI 6.9）蛋白在经ABA处理材料、种胚和白色干燥性愈伤组织中检测到表达，在未经ABA处理的材料中没有检测到表达。推测这两个蛋白可能在胚（或不定胚）的形成过程中起重要作用。

关键词: 水稻, 愈伤组织, 脱落酸, 特异诱导蛋白