Abstract: Sporopollenin behavior during the process of tapetal disintegration of rice cultivar M-101 and its induced genetic male sterile(GMS) no pollen mutant, line Ⅰ-15, was observed by using the yellow fraction of pyronin B to specifically induce the sporopollenin to fluoresce. In cultivar M-101, the process of tapetal disintegration might be divided into two phases: secreting sporopollenin at microspore developmental stage; and degrading non-sporopollenin substances at pollen developmental stage. In line Ⅰ-15, although a series of abnormalities occurred during the process of tapetal disintegration, both the timing of the beginning and the phase sequence of tapetal disintegration were similar to those of cultivar M-101. The events of sporopollenin behavior during the process of tapetal disintegration and their relations to pollen development are discussed.

Key words: Rice genetic male sterility, Tapetal disintegration, Sporopollenin behavior

摘要： 利用派罗宁B-黄色组分诱导孢粉素产生荧光的特异性,观察了水稻M 101品种以及它的无花粉型胞核雄性不育(GMS)突变体Ⅰ-15系花药绒毡层解体过程中孢粉素的行为。M-101品种花药绒毡层的解体过程大体可分为两个阶段:在小孢子发育期,绒毡层主要分泌孢粉素;在花粉发育期,绒毡层主要降解非孢粉素物质。Ⅰ-15系花药绒毡层的解体过程虽然出现了一系列异常现象,但绒毡层解体的起始时间和阶段顺序性与M-101品种相似。对绒毡层解体过程孢粉素行为的有关事态及其与花粉发育的关系等问题进行了讨论。

关键词: 稻胞核雄性不育性, 绒毡层解体, 孢粉素行为