Mapping of FertilityRestoring Gene for WildAbortive Type Cytoplasmic Male Sterility in a japonica Rice Restorer Line SWR78

doi:10.3969/j.issn.1001-7216.2009.04.07

Abstract

Abstract: According to the fertility segregation of F2 and BC1F1 populations derived from a cross between Suqiu A with wildabortive type cytoplasmic male sterility (CMS) and SWR78(a japonical rice restorer line), it could be presumed that the fertility restoration of this cross was controlled by three genes. Then, individuals with absolutely abortive pollen of F2 population were used to map the fertilityrestoring gene. The Rf4 gene was mapped on the long arm of chromosome 10 flanked by the markers RM5629, RM5373, STS1017 and STS1018 at the distances of 0.17, 0.03, 0.03 and 0.07 cM, respectively. The Rf4 gene was localized between RM5373 and STS1017, and the physical distance of the interval between the two markers was about 78 kb.

Key words: japonica rice, cytoplasmic male sterility, fertilityrestoring gene, molecular mapping

摘要： 利用野败(WA)型粳稻广亲和不育系苏秋A和广亲和广谱型恢复系SWR78配组，根据F2与BC1F1群体的育性分离情况，初步推测WA型苏秋A的育性恢复至少由3对基因控制。选取F2群体中无染色花粉植株，采用隐性基因组分析法进行恢复基因定位，将其中1个主效基因Rf4定位于第10染色体长臂上，与标记RM5629、RM5373、STS1017和STS1018分别相距0.17、0.03、0.03和0.07 cM。Rf4位于标记RM5373与STS1017之间，两标记间的物理距离为78 kb。

关键词: 粳稻, 细胞质雄性不育, 恢复基因, 基因定位

ZHANG Honggen,ZHU Zhengbin,LI Bo,LIU Chao,TANG Shuzhu*,LIANG Guohua,GU Minghong. Mapping of FertilityRestoring Gene for WildAbortive Type Cytoplasmic Male Sterility in a japonica Rice Restorer Line SWR78 [J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2009.04.07 .

张宏根,朱正斌,李波,刘超,汤述翥,梁国华,顾铭洪. 粳稻野败型细胞质雄性不育恢复系SWR78的恢复基因定位[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2009.04.07 .

References

[1]曾千春, 周开达, 朱祯, 等. 中国水稻杂种优势利用现状. 中国水稻科学, 2000, 14(4): 243-246.
[2]汤述翥, 张宏根, 孔宪旺, 等. 水稻广亲和广谱型恢复系SWR78的选育. 作物学报, 2007, 33(10): 1567-1574.
[3]Roger S D, Bendich A J. Extraction of DNA from plant tissue. Plant Mol Biol, 1988, A6: 1-10.
[4]Yao F Y, Xu C G, Yu S B, et al. Mapping and genetic analysis of two fertility restorer loci in the wildabortive cytoplasmic male sterility system of rice (Oryza sativa L.). Euphytica, 1997, 98: 183-187.
[5]杨仁崔, 卢浩然. 水稻恢复系IR24恢复基因的初步分析. 作物学报, 1984, 10(2): 81-86.
[6]胡锦国. 水稻雄性不育性遗传分析指标的探讨. 华中农学院学报, 1983, 2(3): 17-20.
[7]张桂权, 卢永根. 水稻质核互作孢子体雄性不育性的基因分析. 作物学报, 1987, 13(1): 23-28.
[8]梁康迳, 王乃元, 杨仁崔. 杂交籼稻恢复性基因的遗传及等位分析. 福建农业学报, 1990, 19(4): 397-402.
[9]滕利生, 申宗坦. 水稻胞质不育的恢复基因分析. 作物学报, 1996, 22(2): 142146.
[10]何光华, 朱云发, 裴炎, 等. 利用极大似然法分析野败型杂交籼稻恢复基因的遗传. 西南农业大学学报, 2000, 13(2): 14-18.
[11]严菊强, 薛庆中. 水稻广亲和性和胞质雄性不育恢复性的遗传分析. 遗传学报, 1995, 22 (5): 361-371.
[12]雷捷成．野败型水稻雄性不育隐性恢复基因及其在育种实践中的意义. 福建农业科技, 1983(5): 16-19.
[13]周天理, 沈锦华, 叶复初. 野败型杂交籼稻的育性基因分析. 作物学报, 1983, 9(4): 241-247.
[14]章旺根, 申宗坦. 水稻品种育性恢复基因的分析. 作物学报, 1987, 13(2): 97101.
[15]Ramalinggam J, Nadarajan N, Varniarajan C, et al. Allelic relationship among fertilityrestoring genes in rice. IRRN, 1995, 20(3): 5-6.
[16]Raj K G, Virmani S S. Genetics of fertility restoration of ‘WA’ type cytoplasmic male sterility in rice. Crop Sci, 1988, 28: 787-792.
[17]Zhang G, Bharaj T S, Lu Y, et al. Mapping of the Rf 3 nuclear fertility restoring gene for WA cytoplasmic male sterility in rice using RAPD and RFLP markers. Theor Appl Genet, 1997, 94: 27-33.
[18]张群宇, 刘耀光, 张桂权, 等. 野败型水稻细胞质雄性不育恢复基因Rf4的分子标记定位．遗传学报, 2002, 29(11): 1001-1004.
[19]Tan X L, Vanavichit A, Amornsilpa S, et al. Genetic analysis of rice CMSWA fertility restoration based on QTL mapping. Theor Appl Genet, 1998, 97(5/6): 994-999.
[20]景润春, 何予卿, 黄青阳, 等. 水稻野败型细胞质雄性不育恢复基因的ISSR和SSLP标记分析. 中国农业科学, 2000, 33(2): 10-15.
[21]何光华, 王文明, 刘国庆, 等. 利用SSR标记定位明恢63的2对恢复基因. 遗传学报, 2002, 29(9): 798-802.
[22]Bharaj T S, Virmani S S, Khush G S. Chromosomal location of fertility restoring genes for ‘wide abortive’ cytoplasmic male sterility using primary trisomics in rice. Euphytica, 1995, 83: 169-173.
[23]李平, 周开达, 陈英, 等. 利用分子标记定位水稻野败型核质互作雄性不育恢复基因. 遗传学报, 1996, 23(5): 357-362.
[24]庄杰云, 樊叶杨, 吴建利, 等. 水稻CMSWA育性恢复基因的定位. 遗传学报, 2001, 28(2): 129-134.
[25]Ahmadikhah A, Karlov G I. Molecular mapping of the fertilityrestoration gene Rf4 for WAcytoplasmic male sterility in rice. Plant Breeding, 2006, 125(4): 363-367.
[26]Liu X Q, Xu X, Tan Y P, et al. Inheritance and molecular mapping of two fertilityrestoring loci for Honglian gametophytic cytoplasmic male sterility in rice (Oryza sativa L.). Mol Gen Genom, 2004, 271: 586-594.