Abstract: One hundred RAPD primers were used to amplify true and false hybrid rice ⅡYou 63 genomic DNAs in this experiment. The main DNA polymorphisms from the two materials were not found, indicating that true and fasle ⅡYou 63 genetic background are similar. However, we had established a PCR system which can be used to determine the seed purity of Shanyou 63 by using a primer P18 to amplify a specific band of 0. 8 kb from restorer line Minghui 63. This technique can also be used to determine true and false ⅡYou 63 correctly and fast, verifying that the Shanyou 63 PCR system is suitable to determine true and false hybrid rices using Minghui 63 as restorer line. ReplaceFont('ChDivSummary','真、假杂交水稻Ⅱ优６３的ＲＡＰＤ鉴定钱前，陈洪，孙宗修，朱立煌（中国水稻研究所，杭州３１０００６）（中国科学院遗传研究所，北京１００１０１）关键词；杂交水稻，ＲＡＰＤ标记，真假鉴定ＴｈｅＳｔｕｄｙｏｎＤｅｔｅｒｍｉｎｉｎｇＴｒｕｅａｎｄＦａｌｓｅＨｙ...');ReplaceFont('EnDivSummary','One hundred RAPD primers were used to amplify true and false hybrid rice ⅡYou 63 genomic DNAs in this experiment. The main DNA polymorphisms from the two materials were not found, indicating that true and fasle ⅡYou 63 genetic background are similar. However, we had established a PCR system which can be used to determine the seed purity of Shanyou 63 by using a primer P18 to amplify a specific band of 0. 8 kb from restorer line Minghui 63. This technique can also be used to determine true and false ⅡYou 63 correctly and fast, verifying that the Shanyou 63 PCR system is suitable to determine true and false hybrid rices using Minghui 63 as restorer line.');if(document.getElementById('ChDivSummary') != null && document.getElementById('ChDivSummary').innerHTML!=""){CutSpan('ChDivSummary',500);DisplaySpanDiv('ChDivSummary');ClearSummaryOnLoad('SummaryLinkChID','SummaryLinkEnID');} if(document.getElementById('EnDivSummary') != null && document.getElementById('EnDivSummary').innerHTML!=""){CutSpan('EnDivSummary',1000);DisplaySpanDiv('EnDivSummary');ClearSummaryOnLoad('SummaryLinkEnID','SummaryLinkChID');}ReplaceChar1('ChDivSummary');ReplaceChar('ChDivSummary');ReplaceChar1('EnDivSummary');ReplaceChar('EnDivSummary');

Key words: determining true and false, hybrid rice , Random Amplified Polymorphic DNA marker, seed authentication

摘要： 以鉴定杂交水稻汕优63种子纯度PCR技术体系为基础，对也采用明恢63为父本的杂交水稻Ⅱ优63与在生产上造成严重损失的假冒Ⅱ优63进行了分子标记鉴定。

关键词: 杂交水稻, RAPD标记, 真假鉴定