Abstract: The effects of hormone and colchlcine on the induction, differentiation, propagation and chromosome doubling of haploid somaclonal micro-buds of indica rice were studied. The results indicated that: 1)2 mg/L of 2,4-D can enhance the culture ability of haploid indica rice. Higher or lower concentration of 2, 4-D can enhance the frequency of induction or differentiation, but reduce the ratio of micro-buds. 2)NAA has distinct effect on the propagation of indica haploid micro-buds. 0.5 mg/L of NAA can propagate micro-buds quickly, the number of micro-buds is 46.21 times of original ones after 35 days, which is 2.8 times of control without NAA, and the weight per micro-bud is only 0.079 mg. The acceptable culture medium to propagate micro-buds is N6 appended with 2.5 mg/L MET, 0.5 mg/L NAA and 2 mg/L 6-BA. 3) Indica haploid micro-buds treated for 48 h with colchiclne at 500mg/L can obtain higher diploid ratio. Its green plantlets ratio and diploid plantlets ratio is 42.9% and 60.0% respectively. Treatment of colchicine is uncalled-for to the callus. Although the treatment of colchiclne can enhance the diploid plantlets ratio from haploid micro-buds, but the total diploid plantlets ratio is lower than the control because of decreasing of differentiation frequency from callus.

Key words: colchicine, haploid, hormone, micro-bud, somaclonal line

摘要： 以籼稻单倍体无性系微芽为材料，研究了激素及秋水仙碱等处理对籼稻单倍体微芽的诱导、分化、扩增及加倍的影响。2 mg/L的2,4-D可提高籼稻单倍体微芽的培养力。NAA对籼稻单倍体微芽的扩增有明显影响，0.5 mg/L的NAA可成倍扩增微芽，培养35 d后，其芽数比原始芽数增加了46.21倍，比不加NAA的对照增加了2.8倍，且单芽重仅0.079 mg。籼稻单倍体微芽扩增的适宜培养基为：N6附加MET 2.5 mg/L、NAA 0.5 mg/L、6-BA 2 mg/L。500 mg/L秋水仙碱溶液处理籼稻单倍体微芽48 h，其二倍体得率较高，绿苗率及二倍体率分别为42.9%和60.0%；秋水仙碱处理愈伤组织的效果不佳，虽然提高秋水仙碱处理浓度可提高二倍体率，最高可达100%，但由于绿苗分化率下降，使总的二倍体得苗率比不处理的对照低。

关键词: 单倍体, 体细胞无性系, 微芽, 组织培养, 激素, 秋水仙碱