Abstract: Anther mRNAs of cytoplasmic male sterile (CMS) rice and its maintainer lines of Yuetai were studied by DDRT-PCR. The cDNA bands showed very little difference between CMS rice and its maintainer lines. Similar results had been obtained at different developmental stages of both CMS and its maintainer lines. These resultsindicated that most of the genes were constitutively expressed. About 24 differential bands were found, AGCK-20 only existed in CMS when hybridized with total RNA by means of dot blotting.

Key words: anther, cytoplasmic male sterility, developmental stage, display, RNA

摘要： 应用DDRT-PCR技术分析了粤泰不育系及保持系单核、二核及三核期的mRNA。不育系和保持系本身不同发育时期花药cDNA扩增带型相似，不育系和保持系同时期花药的cDNA扩增带型也很相似，说明大多数基因都是组成型表达的。在实验中共观察到24条差异带，通过与总RNA斑点杂交对部分差异带初步筛选，表明AGCK-20仅与不育系总RNA有杂交信号。

关键词: 雄性不育, 花药, 差异展示, 发育时期