Improving   Resistance of a GoodQuality japonica Variety Wuyujing 3 to Rice Stripe Virus via Molecular MarkerAssisted Selection

Abstract

Abstract: A molecular markerassisted selection strategy was used to improve the resistance of Wuyujing 3 to rice stripe disease(RSD). A resistant variety Dular in which two linked QTLs qSTV11b and qSTV11c for the resistance to RSD on chromosome 11 were identified in the former research was used as the resistant donor parent and Wuyujing 3 as the recurrent parent. From BC1F1, all the individuals were analyzed with four markers anchoring qSTV11b and qSTV11c. The individuals with the bands from Dular were selected for continual backcrossing. From BC2F1, the individuals with the bands of the four markers from Dular was selfed. From BC2F2 the progeny with homologous markers as Dular was harvested. By the dynamic clustering analysis of the backcross progeny, 9 individuals of BC3F2 generation, 31 individuals of BC4F1 generation and Wuyujiang 3 were grouped together. Finally, a number of lines carrying the diseaseresistance genes have been obtained. Two BC2F3 lines with homozygous diseaseresistance genotype, which have excellent agronomic characters and output and quality characters have also been obtained.

Key words: japonica rice, rice stripe disease, resistance, breeding, molecular marker assisted selection

摘要： 在对抗水稻条纹叶枯病品种Dular进行抗性基因定位的基础上，以它为抗性供体亲本，武育粳3号为抗性受体亲本，通过杂交和回交，从BC1F1开始利用与条纹叶枯病抗性QTL qSTV11b和 qSTV11c 紧密连锁的4个分子标记进行辅助选择，改良武育粳3号的条纹叶枯病抗性和选育抗病新品种。对检测到抗病基因的回交后代进行主要性状的聚类分析，分别有9个抗病基因纯合的BC3F2单株和31个抗病基因杂合的BC4F1单株与武育粳3号聚为一类。经进一步纯合稳定，有望筛选到抗性得到改良、性状回复到武育粳3号的株系。对18个抗病基因纯合的BC2F3株系进行鉴定，其条纹叶枯病的抗性均达到抗的水平，有5个株系丰产性较武育粳3号有明显提高，其中2个株系外观品质也较武育粳3号有了明显改进，达到国家优质稻谷标准。

关键词: 粳稻, 条纹叶枯病, 抗性, 育种, 分子标记辅助选择

ZHANG Honggen#,LI Bo#,ZHU Zhengbin,CUI Xiaofen,TANG Shuzhu*, LIANG Guohua, GU Minghong. Improving Resistance of a GoodQuality japonica Variety Wuyujing 3 to Rice Stripe Virus via Molecular MarkerAssisted Selection [J]. Chinese Journal of Rice Science.

张宏根, 李波, 朱正斌, 崔小芬, 汤述翥, 梁国华, 顾铭洪. 分子标记辅助选择改良武育粳3号的条纹叶枯病抗性[J]. 中国水稻科学.

References

[1]南京农业大学. 农业植物病理学. 南京: 江苏科学技术出版社, 1996: 102-104.

[2]Toriyama S. An RNAdependent RNA polymerase associated with the filamentous nucleoproteins of rice stripe virus. J Gen Virol, 1986, 67: 1247-1255.

[3]朱美凤, 肖庆璞, 王法明, 等. 江南稻区新发生的几种稻病害. 植物保护学报, 1964, 2(3): 100-102.

[4]林奇英, 谢联辉, 周仲驹, 等. 水稻条纹叶枯病的研究:Ⅰ.病害的分布和损失. 福建农学院学报, 1990, 19(4): 421-425.

[5]谢联辉, 魏太云, 林含新, 等. 水稻条纹病毒的分子生物学. 福建农业大学学报, 2001, 30(3): 269-279.

[6]杨荣明, 刁春友, 朱叶芹. 江苏省水稻条纹叶枯病上升原因及防治对策. 植保技术与推广, 2002, 22(3): 9-10, 12.

[7]周彤, 吉健安, 周益军, 等. 水稻品种(系)田间抗条纹叶枯病的鉴定初报. 江苏农业学报, 2006, 22(4): 468-469.

[8]罗兵前, 周林杰, 顾磊. 对水稻条纹叶枯病防治策略的再认识. 江苏农业科学, 2007(1): 73-75.

[9]吴书俊. 水稻抗条纹叶枯病的基因定位及其基因工程育种[D]. 扬州: 扬州大学, 2005.

[10]王才林. 江苏省水稻育种与生产现状及发展趋势. 江苏农业科学, 2005 (2): 1-6.

[11]张坚勇, 万向元, 肖应辉, 等. 水稻品种食味品质性状稳定性分析. 中国农业科学, 2004, 37(6): 788-794.

[12]Roger S D, Bendich A J. Extraction of DNA from plant tissue. Plant Mol Biol, 1988, A6: 1-10.

[13]Washio O, Ezuka A, Toriyama K, et al. Testing method for genetics of and breeding for resistance to rice stripe disease. Bull Chugoku Agric Exp Sta Series, 1968, 16: 39-197.

[14]朱满山, 顾铭洪, 汤述翥. 不同粳稻品种和DH群体稻米淀粉RVA 谱特征与蒸煮理化指标及相关分析. 作物学报, 2007, 33(3): 41-418.

[15]薛庆中, 张能义, 熊兆飞, 等. 应用分子标记辅助选择培育抗白叶枯病恢复系. 浙江农业大学学报, 1998, 24(6): 581-582.

[16]曹立勇, 庄杰云, 占小登, 等. 抗白叶枯病杂交水稻的分子标记辅助育种. 中国水稻科学, 2003, 17(2): 184-186.

[17]黄廷友, 李仕贵, 王玉平, 等. 分子标记辅助选择改良蜀恢527 对白叶枯病的抗性. 生物工程学报, 2003, 19(2): 153-157.

[18]彭应财, 李文宏, 樊叶杨, 等. 利用分子标记辅助选择技术育成抗白叶枯病杂交稻协优218. 杂交水稻, 2003, 18(5): 5-7.

[19]杨子贤, 姜恭好, 徐才国, 等. 利用分子标记辅助选择改良 9311 对白叶枯病和螟虫抗性. 分子植物育种, 2004, 2(4): 473-480.

[20]罗彦长, 王守海, 李成荃, 等. 分子标记辅助选择抗白叶枯病基因在选育水稻广亲和恢复系上的应用. 中国水稻科学, 2005, 19(1): 36-40

[21]李仕贵, 王玉平, 黎汉云, 等. 利用微卫星标记鉴定水稻的稻瘟病抗性. 生物工程学报, 2000， 16(3): 324-327.

[22]刘士平, 李信, 汪朝阳, 等. 利用分子标记辅助改良珍汕97的稻瘟病抗性. 植物学报, 2003, 45(11): 1346-1350.

[23]陈学伟, 李仕贵, 马玉清, 等. 水稻抗稻瘟病基因Pi-d(t)1、Pi-b、Pi-ta2的聚合及分子标记选择. 生物工程学报, 2004, 20(5): 708-714.

[24]陈志伟, 官华忠, 吴为人, 等. 稻瘟病抗性基因Pi1连锁SSR标记的筛选和应用. 福建农林大学学报:自然科学版, 2005, 34(1): 74-77.

[25]Washio O, Ezuka A, Sakurai Y, et al. Studies on the breeding of rice varieties resistant to stripe disease:Ⅰ. Varietal difference in resistance to stripe disease. Jpn J Breeding, 1967, 17: 91-98.

[26]Washio O, Ezuka A, Sakurai Y, et al. Studies on the breeding of rice varieties resistant to stripe disease: Ⅲ. Genetic studies on resistance to stripe disease in foreign varieties. Jpn J Breeding, 1968, 18: 167-172.

[27]Maeda H, Sugisawa T, Yoshihiro S. QTL analysis for rice stripe resistance in the Japanese upland rice Kanto. Breeding Sci, 2004, 54: 19-26.

Improving Resistance of a GoodQuality japonica Variety Wuyujing 3 to Rice Stripe Virus via Molecular MarkerAssisted Selection

分子标记辅助选择改良武育粳3号的条纹叶枯病抗性

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