Abstract: Rice uncoated seeds were soaked in pBI121 plasmid solution, and screened in G418 solution. The rice seedlings resistant to G418 were got. Southern blot analysis indicates that NPT Ⅱ gene in pBI121 integrated into the genome of rice acceptor. By means of scanning electronic microscope, the surface of cell in apical point show that there were different microstructures between materials soaked in DNA solution and controls, which means that special gene expression must have been induced by DNA molecules.

Key words: cell surface, gene transfer, microscope, molecular cross

摘要： 以pBI121质粒作外源DNA，去壳水稻种子为浸泡受体。对G418抗性苗进行Southern blot分析，证实pBI121上的NPTⅡ基因整合到受体基因组中。以溶解pBI121的TE溶液浸泡处理为对照，用扫描电镜观察胚芽生长点外层细胞表面，结果显示，对照与DNA溶液浸泡组两者间细胞表面结构有相似性，但DNA溶液浸泡组出现对照组所没有的表面孔洞结构，说明DNA可诱导生长点细胞表面产生新的结构。

关键词: 细胞表面, 基因转移, 显微观察, 分子杂交