Abstract: Experiment on microelements found that copper was the only element responsible for the pronounced effect of enhancement on plant regeneration in rice callus culture. By supplement of copper to the medium, the plant regeneration frequency, the number and the weight of the regenerated plants were evidently increased; the effective concentration range of copper to be used is a broad one. When surveying calli of other varieties using copper at 5 μmol/L, eight out of 15 showed positive response above significant level. Results of the present experiments indicate that raising copper concentration of the medium is a simple and effective method for the enhancement of plant regeneration in rice callus culture.

Key words: callus culture, plant regeneration, copper

摘要： 在培养基中添加各常用微量元素的实验结果表明，只有铜元素具有明显的促进水稻愈伤组织植株再生的作用。铜元素不仅能提高再生植株频率和数量，还能提高再生植株的重量，其作用浓度的范围相当宽广。采用添加5 μmol/L铜元素的方法调查另外15个品种的培养效果，发现其中有8个对铜元素的正向反应达到了统计学上的显著水平。据此认为，提高培养基中铜元素浓度是一种增强水稻愈伤组织植株再生的简单和有效的方法。

关键词: 组织培养, 植株再生