Abstract: The effects of sorbitol and mannitol as carbon source on callus induction, plantlet regeneration and haploid chromosome doubling in diploid and haploid young panicle culture of indica rice line TC90 were studied. The results showed that 2% or 4% sorbitol as medium carbon source could efficiently maintain explants alive at least for 25 days, and plantlet regeneration frequency reached 52. 6% and 90. 0% respectively, and haploid doubling rate reached as high as 30. 5%. On the contrary, the explants did not produce any callus, became pale, shrunk and died out after being cultured seven days when 2%or 4% mannitol used as carbon source.

Key words: sorbitol, auto-doubling, somatic cell culture, rice

摘要： 山梨醇和甘露醇作碳源，对籼稻品系TC90及其花培单倍体幼穗愈伤组织诱导、植株分化与染色体自然加倍的研究结果表明，2%和4%的山梨醇作唯一碳源时，可维持外植体生存25 d以上，绿苗再生频率分别为52.6%和90.0%，单倍体自然加倍率达30.5%。2%或4%的甘露醇作碳源时，外植体均不能生长，逐渐变得苍白、干瘪，培养7 d后失去分化再生能力。

关键词: 山梨醇, 自然加倍, 体细胞培养, 水稻