Chinese Journal of Rice Science

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Microbial Diversity of Nonpathogenic Pseudomonads and Related Bacteria from Rice Seeds in Zhejiang Province of China and Luzon Island of the Philippines (in English)

Xie Guanlin; Wu Zhixian; Yu Xuefang; T W Mew   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-10-10 Published:1999-10-10

菲律宾吕宋岛和中国浙江省的稻种非致病假单胞杆菌及相关细菌多样性研究

谢关林1;吴芝仙2;余雪芳1;苗东华3   

  1. 1浙江大学 植物保护系,中国 浙江 杭州 310029; E-mail:glxie@zjau.edu.cn; 2 浙江大学 动物科学学院,中国 浙江 杭州 310029;3 国际水稻研究所 昆虫和植物病理学部,菲律宾马尼拉933信箱

Abstract: One hundred and sixteen seed samples from Zhejiang Province of China and 129 from Luzon Island of the Philippines were studied for species diversity of Pseudomonads and related bacteria during 1993 - 1998. Over 1200 bacterial isolatesfrom Zhejiang Province and 2300 from Luzon Island were picked and identified by bacteriology, pathogenicity and numeric taxonomy method (Biolog). The fluorescent bacteria population in unit of rice seed from Luzon island was significantly higher thanthat from Zhejiang Province. Sixteen species or types of Pseudomonas and two related species were identified, about half of which were not recorded from rice seeds before. Five hundreds and three nonpathogenic Pseudomonads and related bacterial isolates from Zhejiang Province and Luzon Island were tested for antagonists against Rhizoctonia solani, Sarocladium oryzae, and Acidovorax avenae subsp. avenae. About 12% and 17% of the total isolates were antagonistic against one or more of the pathogens in Zhejiang Province and Luzon Island respectively.

Key words: bacterial diversity, Pseudomonas, rice , seed, bacterial population, biological control

摘要: 在1993~1998年间对采自中国浙江省的116批及菲律宾吕宋岛的129批稻种进行了假单胞杆菌及相关细菌多样性研究。分别从浙江省和吕宋岛分离出1200多个和2300多个有关菌株,并对它们进行了细菌学、致病性和数值分类(Biolog)法测定。结果显示,吕宋岛稻种上的荧光细菌数明显高于浙江省。鉴定出16个假单胞杆菌种或型及2个相关种,其中约一半的细菌种尚未有从稻种上分离出来的记录。503个菌株对水稻纹枯病菌、水稻叶鞘腐败病菌及水稻细菌性褐条病菌的拮抗性测定表明,在浙江省约占稻种细菌总数12%的菌对一个或多个水稻病原菌具拮抗性,而在吕宋岛约占17%左右。

关键词: 细菌多样性, 假单胞杆菌, 水稻, 种子, 细菌群体, 生物