Abstract: Gene expression patterns in rice elongation induced by GA₃ were investigated by using mRNA differential display technique. From 100 combinations of anchor and arbitrary primers, twenty one GA₃ induced cDNA fragments were obtained. One of them was sequenced and shown to encode extensin like protein. Quantitative PCR confirmed that the expression of GA15b was induced by GA₃. Southern blot analysis indicated that the GA15b was a multicopy gene.

Key words: Oryza sativa, gibberellin, extensin like protein, gene

摘要： 采用mRNA 差异显示技术对受GA₃ 诱导的水稻伸长相关基因进行了分离和鉴定。经100 个引物组合差异显示,在获得21 个受GA₃ 诱导表达的cDNA 片段的基础上, 对其中的GA 15b 片段克隆进行了序列测定。其序列经国际联网BLAST 查询表明GA 15b 为伸展蛋白类似蛋白的候选基因片段。定量PCR 进一步确证GA 15b 受GA₃ 所诱导。Southern 杂交表明GA 15b 以多拷贝形式存在。

关键词: 水稻, 赤霉素, 伸展蛋白, 基因