Abstract: By using 15 microsatellite DNA primers, Simple Sequence Length Polymorphism(SSLPs) of 13 rice genotypes were analyzed and detected with different methods. The results show that denaturing polyacrylamide electrophoresis including isotopic labelling and automated fluorescence is more effective than electrophoresis on 3% MetaPhor agarose gel . The advantage of the automated fluorescence system as compared to standard [ ³²P] labelling sequencing gels are: (1) several bases resolving clearly ; (2) the ability to visualize and analyze by several products in the same lane within the same size range, but with different fluorescent labels; (3)automated sizing; (4)automated data output; and (5)elimination of radioactivity. These advantages increase the efficiency and rapidity of rice SSLPs analysis.

Key words: rice, simple sequence repeat, polymorphic detection, research method

摘要： 用15对水稻微卫星引物，对13个不同类型及来源的水稻基因型材料进行了简单序列重复片段长度的多态性(SSLPs)分析，并与不同的检测和分析方法进行了比较。聚丙烯酰胺凝胶电泳无论是放射性自显影分析还是荧光标记的自动测序仪分析都比高密度的琼脂糖电泳(3% MetaPhor agarose)有效。利用荧光标记自动测序系统的SSLPs分析较之传统的32P标记的聚丙烯酰胺凝胶电泳分析，具有以下优点：(1)借助于荧光染料标记的丰富内标，可以区别几个bp之差的SSLPs ；(2)借助于不同的荧光标记可以在同一样孔里分析多个样品；(3)自动给出SSLPs片段的长度；(4)自动的数据输出和分析；(5)避免了放射性同位素有可能造成的环境污染。因此，该方法具有快速、高效的特点。

关键词: 水稻, 简单序列重复, 多态性检测