Abstract: Using 72 RFLP markers distributed on 12 chromosomes together with 8 restriction enzymes the RFLPs among a rice variety, Kinmaze and its giant embryo and sugary endosperm mutants derived from MNU treatment were studied. The results indicated that RFLPs between ge mutant and Kinmaze, su mutant and Kinmaze, and ge and su mutants were 8.3%, 5.6% and 12.5%, respectively. The mutation frequencies in ge and su mutant were 0.0227 and 0.0070 respectively. Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation.

Key words: rice, giant embryo, sugary endosperm, mutant, restriction fragement length polymorphism, molecular mapping

摘要： 利用均匀分布于水稻12条染色体上的72个RFLP标记与8种限制性内切酶（EcoR Ⅰ、EcoRⅤ、HindⅢ、Dra Ⅰ、ScaⅠ、Xba Ⅰ、BamH Ⅰ 和BglⅡ）的不同组合，对化学诱变剂MNU诱变产生的巨大胚、甜胚乳两个突变体及其原始品种金南风三者之间进行RFLP分析。结果表明：巨大胚突变体与金南风、甜胚乳突变体与金南风以及两突变体之间的多态性分别为8.3%、5.6%和12.5%。巨大胚和甜胚乳两突变体基因突变频率分别为0.0227和0.0070。根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。

关键词: 水稻, 巨大胚, 甜胚乳, 突变体, 限制性片段长度多态性, 分子标记