Abstract: By using mRNA differential display, gene expression patterns in rice induced by plant hormone-gibberellin were investigated. From 50 combinations of anchor and arbitrary primers, twenty one tagged cDNA fragments were obtained and screened the fragments by reverse-Northern. Five positive cDNA fragments were cloned and sequenced. One of which was shown to encode sequences for rice chloroplast ATP synthase. Northern blot analysis indicated that the upregulation of this gene occurs at the transcriptional level in rice after gibberellin treatment for 16 h, suggesting that chloroplast ATP synthase may play a role in rice response to gibberellin.

Key words: Oryza sativa, gibberellin, chloroplast, ATP synthase, gene, cloning

摘要： 采用mRNA差异显示技术分离和鉴定了水稻受赤霉素诱导的差异表达基因。经50个引物组合差异显示，获得21个诱导表达差异的cDNA片段。经反向Northern初步筛选对其中5个阳性片段进行克隆及序列分析。其序列经国际联网BLAST查询表明编号为GA21C的为水稻叶绿体ATP合成酶基因片段。Southern杂交结果证实此基因为单拷贝。Northern杂交结果显示确受赤霉素诱导表达且在诱导16 h后达到高峰，表明赤霉素诱导水稻产生生理反应过程涉及叶绿体基因表达。

关键词: 水稻, 赤霉素, 叶绿体, ATP合成酶