Abstract: Quantitative trait loci (QTLs) affecting total leaf number of the main stem, leafing rate at two different growth stages, heading date, and plant height in a recombinant inbred lines (RIL)population from the cross Lemont (japonica)×Teqing (indica) were investigated using a complete RFLP map with 182 marker loci. Eight QTLs for rate of leaf growth at two different stages, two QTLs for total leaf number of main stem, three QTLs for heading date, and four QTLs for plant height were mapped. Combined with previous studies on this cross, these results provided useful information on the nature of some detected QTLs. For example, QLn3, a main effect QTL from Teqing that give 1.5 leaf more of total leaf number, 9 days delayed heading, and 0.2 leaf increase each ten days during tillering stage in greenhouse condition was identified on chromosome 3 near the marker RG348. This QTL appeared to affect the basic vegetative period of rice plants since it was not affected by the daylength. On the other hand, QHd8 which affects heading date, plant height, and total leaf number of main stem appeared to be influenced by daylength.

Key words: recombinant inbred line, leafing rate, total leaf number, gene location, rice

摘要： 用一张具有182个RFLP标记的分子连锁图谱和一套重组自交系(RIL)群体，对水稻植株主茎总叶片数、叶片生长速率、抽穗期和株高等数量性状进行QTL区间作图研究，定位了影响水稻出叶速率的8个QTL，主茎总叶片数的2个QTL、抽穗期的3个QTL和株高的4个QTL。对这些QTL的遗传效应分析并结合先前用同组合材料研究结果，为进一步明确一些QTL的基因功能提供了有用的信息。如控制水稻主茎总叶片数的一个主效QTL，即QLn3，它同时影响分蘖期出叶速率、抽穗期及株高等数量性状。该位点来自特青的等位基因，其加性效应可使水稻在温室冬季短日条件下主茎总叶片数增加1.5叶左右，抽穗期延迟9 d，同时具有使分蘖期出叶速率降低0.2叶/10 d的效应。由于该QTL位点的基因不受光照长度的影响，说明它们有可能是由一个影响水稻基本营养生长期的基因控制或者这些基因紧密连锁；而另一个QTL（QHd8）位点的基因对主茎总叶数、抽穗期和株高的效应似乎受光照长度的影响较大。

关键词: 重组自交系, 出叶速率, 主茎总叶数, 基因定位, 水稻