Abstract: The RAPD analysis was conducted on the main 31 restorer lines of hybrid rice in China. Out of 48 random primers, 9 primers were screened, then amplified the 31 genomic DNAs. Seventy-eight RAPD fragments were generated in all, 61 of them were polymorphic markers. Every primer offered 8.7 markers on average. The molecular phylogenetic trees constructed by UPMGA method showed the genetic relationship among 31 restorer lines, which was identical with pedigree analysis.

Key words: hybrid rice, random amplified polymorphic DNA, restorer line, polymorphism, genetic relationship

摘要： 利用RAPD引物对我国杂交稻主要的31个恢复系进行了DNA多态性分析。从152个随机引物中筛选出48个引物扩增恢复系的基因组DNA，选取9个引物的扩增结果进行分析，共获得78条带，其中61条带为多态性标记，每个引物平均提供8.7个标记信息。由UPMGA方法得到的聚类分析结果表明了31个恢复系间的亲缘关系，聚类结果与它们的系谱关系基本吻合。

关键词: 杂交水稻, 随机扩增多态性DNA, 恢复系, 多态性, 亲缘关系