Chinese Journal OF Rice Science

Chinese Journal of Rice Science

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cDNA Cloning and Sequence Analysis of Genome Segment S10 from Rice Black-Streaked Dwarf Virus

ZHANG Heng-mu 1; 2; CHEN Jian-ping 2; *; XUE Qing-zhong 1; LEI Juan-li   

  1. (1 College of Agriculture and Biotechnology; Zhejiang University; Hangzhou 310029; China; 2Virology Laboratory; Zhejiang Academy of Agricultural Sciences; Hangzhou 310021)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-01-10 Published:2002-01-10

水稻黑条矮缩病毒基因组片段S10的cDNA克隆及全序列分析

张恒木 1，2；陈剑平 2，*；薛庆中 1；雷娟利 2   

  1. 1 浙江大学 农业与生物技术学院， 浙江 杭州 310029； 2 浙江省农业科学院 病毒学实验室， 浙江 杭州 310021； * 通讯联系人，E-mail:jpchen@mail.hz.zj.cn

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Abstract: Genome segment S10 of a Zhejiang isolate of rice black-streaked dwarf virus (RBSDV-Zj) was amplified by RT-PCR and sequenced. It consisted of 1801 nt (EMBL accession number is AJ297433) with only one open reading frame which encodes a polypeptide with molecular weight of 63.1 kD. Sequence comparison showed 93.8% and 96.2% homology at level of nucleotide and amino acid sequences between RBSDV-Zj and Japanese isolate (RBSDV-Jap), respectively;and shared 87.1% and 92.0% homology at level of nucleotide and acid sequences between RBSDV-Zj and Italian MRDV, respectively.

Key words: rice black-streaked dwarf virus, genome segment, sequence analysis

摘要： 基于RT-PCR策略克隆了水稻黑条矮缩病毒（rice black streaked dwarf virus，RBSDV）浙江分离株基因组S10，并测定了它的全序列。结果表明，水稻黑条矮缩病毒浙江分离物（RBSDV-Zj）基因组片段S10全长1801 nt（EMBL登录号为AJ297433），仅含有一个大的开放阅读框（open reading frame，ORF），编码一个63.1 kD的多肽，与日本的水稻黑条矮缩病毒基因组片段S10在核苷酸和氨基酸水平上同源性分别为93.8%和96.2%；与意大利玉米粗缩病毒（maize rough dwarf virus，MRDV）的基因组片段S10在核苷酸和氨基酸水平上的同源性分别为87.7%和92.0%，推测它们是同一病毒的不同地理小种。

关键词: 水稻黑条矮缩病毒, 基因组片段