Improve the Character of Rice Plants Utilizing Potassium by Overexpressed Foreign Potassium Channel Genes

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Improve the Character of Rice Plants Utilizing Potassium by Overexpressed Foreign Potassium Channel Genes

YAN Wei-dong ¹; WANG Xiao-chang¹; HE Si-jie ²; TANG Li ¹; AN Zhi-zhuang ¹; TIAN Wen-zhong ²; SHI Wei-ming^{1; *}; CAO Zhi-hong¹

(¹ Institute of Soil Science; Chinese Academy of Sciences; Nanjing 210008; China; ²Institute of Genetics; Chinese Academy of Sciences, Beijing 100101, China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-01-10 Published:2002-01-10

利用外源钾通道基因改良水稻钾素营养

严蔚东 1; 王校常 1; 何锶洁 2; 汤利 1; 安志装 1; 田文忠 2; 施卫明 1,*; 曹志洪 1

1中国科学院南京土壤研究所，江苏南京 210008；2 中国科学院遗传研究所，北京 100101；*通讯联系人，E-mail:wmshi@issas.ac.cn

Abstract

Abstract: The potassium channel genes (KAT1 and AKT1) were introduced into the rice (Zhonghua 8, Zhonghua 9, Zhonghua 13 and 8706) by using particle bombardment method. The molecular analysis confirmed the integration and expression of these genes in the transgenic rice plants. The results showed that transgenic plants have the high capacity of accumulating potassium in a pot cultural experiment with two different K levels.

Key words: potassium channel gene, rice, potassium, plant nutrition, gene transfer

摘要： 通过基因枪导入法，将外源钾通道基因 (KAT1和AKT1)导入水稻中花8号、中花9号、中花13以及8706中，获得了转基因植株，经分子检测，证实了钾通道基因在水稻基因组中的整合与表达。盆栽试验表明，转基因植株在低钾和高钾两种钾水平下其钾的累积能力都有明显提高。

关键词: 钾通道基因, 水稻, 钾素, 植物营养

YAN Wei-dong ,WANG Xiao-chang ,HE Si-jie ,TANG Li ,AN Zhi-zhuang ,TIAN Wen-zhong ,SHI Wei-ming ,CAO Zhi-hong . Improve the Character of Rice Plants Utilizing Potassium by Overexpressed Foreign Potassium Channel Genes[J]. Chinese Journal of Rice Science.

严蔚东 , 王校常 , 何锶洁 , 汤利 , 安志装 , 田文忠 , 施卫明 , 曹志洪 . 利用外源钾通道基因改良水稻钾素营养[J]. 中国水稻科学.

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