Chinese Journal of Rice Science

• 实验技术 • Previous Articles     Next Articles

Improvement of AFLP Protocol for Silver-Staining in Rice

ZHU Zheng-ge 1; 3; JIA Ji-zeng 2; SUN Zong-xiu 1   

  1. (1 The Key Laboratory for Rice Biology; Ministry of Agriculture; China National Rice Research Institute; Hangzhou 310006; China; 2The Key Laboratory for Crop Germplasm Resources and Biotechnology, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-01-10 Published:2002-01-10

水稻AFLP指纹银染法显带研究

朱正歌 1,3; 贾继增 2; 孙宗修 1   

  1. 1中国水稻研究所, 农业部水稻生物学重点实验室, 浙江 杭州 310006; 2中国农业科学院 作物品种资源研究所, 农业部作物品种资源与生物技术重点实验室, 北京 100081; 3河北师范大学 生命科学学院, 河北 石家庄 050016

Abstract: Using transgenic rice as materials, the technique of AFLP silver-staining protocol and some skills were specified, and excellent results were obtained.

Key words: amplified fragment length polymorphism, silver-staining, experimental technique

摘要: 以转基因水稻为材料,对AFLP指纹银染技术及实验操作中的关键技巧做了比较详尽的叙述,取得了比较理想的实验结果。

关键词: 随机片段长度多态性, 银染, 实验技术