中国水稻科学

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CRMS诱导水稻雄性不育的研究:Ⅲ .CRMS对水稻花药游离脯氯酸含量的影响及其与花药败育的关系

俞美玉;王 熹;陶龙兴;姚福德   

  1. 中国水稻研究所,杭州310006
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1991-12-05 发布日期:1991-12-05

The Male Sterility in Rice Plant Induced by CRMS: Ⅲ. Effect of CRMS on free proline content in anthers of rice and its possible relation to abortion of anther

Yu Meiyu; Wang Xi; Tao Longxing; Yao Fude   

  • Received:1900-01-01 Revised:1900-01-01 Online:1991-12-05 Published:1991-12-05

摘要: 利用3H-脯氟酸、3H-谷氨酸示踪分析方法,研究了CRMS诱导水稻雄性不育的作用机理。试验结果表明,CRMS阻碍剑叶向花药运输脯氨酸,抑制花药本身合成脯氨酸能力,并降低脯氨酸合成醇不溶性大分子物质的结合率,引起花药营养匮乏,导致花药败育。

关键词: 雄性不育, 游离脯氨酸, 谷氨酸, 化学杀雄剂

Abstract: The feeding experiment with 3H-proline and infiltration one with 3H-glutamic acid into anthers were carried out to investige the effects of CRMS on proline transport from flag leaf into anthers, and the transformability of anthers from glutamic acid into proline and the possible cause of anther abortion. The results were obtained as follows: (1) There was significantly difference in the content of free proline in anthers between CK and CRMS treated plant at miosis of pollen mother cell. (2) The free proline in anthers mainly came from the vegetative parts, and this transporting process as an active one, could be inhibited by CRMS. The anther themselves can also transform glutamic acid into proline. These might result in the anther proline deficiency and its abortion.

Key words: Male Sterility, Free proline, Glutamic acid, Chemical hybridizing agent