水稻中八个稻瘟病抗性基因特异分子标记的开发及应用

doi:10.16819/j.1001-7216.2024.230811

中国水稻科学 ›› 2024, Vol. 38 ›› Issue (5): 525-534.DOI: 10.16819/j.1001-7216.2024.230811

水稻中八个稻瘟病抗性基因特异分子标记的开发及应用

杨好¹^,^#, 黄衍焱¹^,^#, 王剑²^,³, 易春霖¹, 石军⁴, 谭楮湉¹, 任文芮¹, 王文明¹^,^*()

¹四川农业大学西南作物基因资源发掘与利用国家重点实验室, 成都 611130
²四川农业大学农学院, 成都 611130
³四川省农业科学院植物保护研究所, 成都 610066
⁴绵阳市农业科学研究院/厅市共建作物特色资源创制及应用四川省重点实验室, 四川绵阳 621023

收稿日期:2023-08-31 修回日期:2023-11-17 出版日期:2024-09-10 发布日期:2024-09-10
通讯作者: *email: j316wenmingwang@163.com
作者简介:^#共同第一作者
基金资助:
国家自然科学基金资助项目(U19A2033);国家自然科学基金资助项目(31901839);四川省青年科技创新研究团队资助项目(2022JDTD0023)

Development and Application of Specific Molecular Markers for Eight Rice Blast Resistance Genes in Rice

YANG Hao¹^,^#, HUANG Yanyan¹^,^#, WANG Jian²^,³, YI Chunlin¹, SHI Jun⁴, TAN Chutian¹, REN Wenrui¹, WANG Wenming¹^,^*()

¹State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China
²College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
³Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
⁴Institute of Rice Research, Mianyang Academy of Agricultural Sciences/Crop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang 621023, China

Received:2023-08-31 Revised:2023-11-17 Online:2024-09-10 Published:2024-09-10
Contact: *email: j316wenmingwang@163.com
About author:^#These authors contributed equally to the work

摘要/Abstract

摘要：

【目的】为了明确水稻亲本的稻瘟病抗性基因组成，并有效地应用于水稻抗性育种，开发不受遗传背景限制的分子标记至关重要。这些分子标记能够精确地识别亲本中的抗性基因，为分子辅助育种提供重要的工具。【方法】发掘抗性基因编码区在155份水稻材料中等位基因的核苷酸多态性位点，在特异性最强位点开发分子标记。【结果】经多重阳性及阴性对照或部分结果的重测序检验，为Pit、Pish、Pib、Pid3、Pi5、Pia、Pi54和Pita2/Ptr等8个抗性基因开发了有效的分子标记，并明确这些抗性基因在109个四川盆地常用育种亲本中的组成。其中，Pia基因不存在于这些亲本中，Pit、Pish、Pi54、Pid3、Ptr/Pita2、Pi5和Pib基因分别存在于3.67%、13.76%、14.68%、18.35%、24.77%、26.61%和38.53%的亲本中。另外，有21.10%的水稻亲本不含这些基因，35.78%的亲本只含有1个抗性基因，43.12%的亲本聚合了2~4个抗性基因。

关键词: 水稻, 稻瘟病, 抗性基因, 分子标记

Abstract:

【Objective】To elucidate the composition of blast resistance genes in rice parents and facilitate their effective utilization in rice resistance breeding, it’s crucial to develop molecular markers that transcend genetic background limitations. These molecular markers can accurately discern resistance genes in parents, serving as essential tools for marker-assisted breeding.【Method】By comparing the coding sequence of each Resistance gene with allelic genes in 155 rice resources, nucleotide polymorphism sites within each resistance gene were identified. Primer pairs for molecular markers were then developed targeting the most specific polymorphism sites.【Results】Effective molecular markers were successfully developed for eight resistance genes: Pit, Pish, Pib, Pid3, Pi5, Pia, Pi54, and Pita2/Ptr. These markers were validated through examination using multiple positive and negative controls or gene sequencing. Subsequently, these markers were utilized to identify the resistance gene composition in 109 breeding parent lines commonly used in the Sichuan Basin. Results revealed that the Pia gene was absent in these parent lines, while Pit, Pish, Pi54, Pid3, Ptr/Pita2, Pi5, and Pib genes were present in 3.67%, 13.76%, 14.68%, 18.35%, 24.77%, 26.61%, and 38.53% of lines, respectively. Furthermore, 21.10% of the rice parent lines lacked these resistance genes, 35.78% possessed one resistance gene, and 43.12% harbored two to four resistance genes.

Key words: rice, rice blast disease, resistance genes, molecular markers

杨好, 黄衍焱, 王剑, 易春霖, 石军, 谭楮湉, 任文芮, 王文明. 水稻中八个稻瘟病抗性基因特异分子标记的开发及应用[J]. 中国水稻科学, 2024, 38(5): 525-534.

YANG Hao, HUANG Yanyan, WANG Jian, YI Chunlin, SHI Jun, TAN Chutian, REN Wenrui, WANG Wenming. Development and Application of Specific Molecular Markers for Eight Rice Blast Resistance Genes in Rice[J]. Chinese Journal OF Rice Science, 2024, 38(5): 525-534.

图/表 7

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编号 No.	水稻品种 Rice variety	编号 No.	水稻品种 Rice variety	编号 No.	水稻品种 Rice variety	编号 No.	水稻品种 Rice variety
1	IRBLa-A (Pi-a)	35	绵恢146 Mianhui 146	69	泰恢808 Taihui 808	103	德香074B Dexiang 074B
2	IRBLi-F5 (Pi-i)	36	绵恢725 Mianhui 725	70	五山丝苗 Wushansimiao	104	赣香B Ganxiang B
3	IRBLks-F5 (Pi-ks)	37	南恢558 Nanhui 558	71	西科恢768 Xikehui 768	105	广8B Guang 8B
4	IRBLk-Ka (Pi-k)	38	南恢968 Nanhui 968	72	雅禾 Yahe	106	泸香618B Luxiang 618B
5	IRBLkp-K60 (Pi-kp)	39	蜀恢257 Shuhui 257	73	雅恢2115 Yahui 2115	107	绵5B Mian 5B
6	IRBLkh-K3 (Pi-kh)	40	蜀恢308 Shuhui 308	74	雅恢2116 Yahui 2116	108	忠香B Zhongxiang B
7	IRBLz-Fu (Pi-z)	41	蜀恢451 Shuhui 451	75	雅恢2117 Yahui 2117	109	内香6B Neixiang 6B
8	IRBLz5-CA (Pi-z5)	42	蜀恢498 Shuhui 498	76	雅恢2118 Yahui 2118	110	千乡654B Qianxiang 654B
9	IRBLzt-T (Pi-zt)	43	蜀恢505 Shuhui 505	77	雅恢2119 Yahui 2119	111	荃9311B Quan 9311B
10	IRBLta-K1 (Pi-ta)	44	蜀恢548 Shuhui 548	78	雅恢2199 Yahui 2199	112	蓉18B Rong 18B
11	IRBLb-B (Pi-b)	45	蜀恢573 Shuhui 573	79	雅恢2275 Yahui 2275	113	蓉7B Rong 7B
12	IRBLt-K59 (Pi-t)	46	蜀恢586 Shuhui 586	80	雅恢2348 Yahui 2348	114	双1B Shuang 1B
13	IRBLsh-B (Pi-sh)	47	蜀恢589 Shuhui 589	81	雅恢2816 Yahui 2816	115	泰丰B Taifeng B
14	IRBL1-CL (Pi-1)	48	猪毛糯 Zhumaonuo	82	雅恢2918 Yahui 2918	116	武金4B Wujin 4B
15	IRBL3-CP4 (Pi-3)	49	HR2168	83	雅恢5049 Yahui 5049	117	武香B Wuxiang B
16	IRBL5-M (Pi-5)	50	R900	84	雅恢5217 Yahui 5217	118	雅1B Ya 1B
17	IRBL7-M (Pi-7)	51	R9311	85	雨恢38 Yuhui 38	119	雅3B Ya 3B
18	IRBL9-W (Pi-9)	52	成恢19 Chenghui 19	86	岳恢9113 Yuehui 9113	120	雅7B Ya 7B
19	IRBL12-M (Pi-12)	53	乐恢188 Lehui 188	87	粤农丝苗 Yuenongsimiao	121	宜香1B Yixiang 1B
20	IRBL19-M (Pi-19)	54	乐恢584 Lehui 584	88	D297B	122	泰国香稻 Thai fragrant rice
21	IRBLkm-Ts (Pi-km)	55	泸恢17 Luhui 17	89	D35B	123	楚粳28 Chujing 28
22	IRBL20-IR24 (Pi-20)	56	泸恢8258 Luhui 8258	90	D62B	124	野香B Yexiang B
23	IRBLta2-Pi (Pi-ta2)	57	绵恢523 Mianhui 523	91	D79B	125	绵恢6139 Mianhui 6139
24	IRBL11-Zh (Pi-11)	58	绵恢528 Mianhui 528	92	F-32B	126	绵恢313 Mianhui 313
25	E33	59	绵恢662 Mianhui 662	93	F59B	127	绵恢357 Mianhui 357
26	成恢3203 Chenghui 3203	60	明恢63 Minghui 63	94	G46B	128	绵恢365 Mianhui 365
27	成恢727 Chenghui 727	61	明恢86 Minghui 86	95	川106B Chuan 106B	129	绵恢821 Mianhui 821
28	成恢993 Chenghui 993	62	内恢99-14 Neihui 99-14	96	川608B Chuan 608B	130	绵恢823 Mianhui 823
29	德恢6099 Dehui 6099	63	蜀恢137 Shuhui 137	97	川谷B Chuangu B	131	绵恢837 Mianhui 837
30	多恢1号 Duohui 1	64	蜀恢208 Shuhui 208	98	川华B Chuanhua B	132	绵恢857 Mianhui 857
31	航恢1179 Hanghui 1179	65	蜀恢527 Shuhui 527	99	川农1B Chuannong 1B	133	绵恢815 Mianhui 815
32	航恢570 Hanghui 570	66	蜀恢538 Shuhui 538	100	川农4B Chuannong 4B	134	日本晴 NPB
33	华占 Huazhan	67	蜀恢569 Shuhui 569	101	川种3B Chuanzhong 3B	135	中花11 Zhonghua 11
34	乐恢433 Lehui 433	68	蜀恢882 Shuhui 882	102	德66B De 66B	136	丽江新团黑谷 LTH

编号 No.	水稻品种 Rice variety	编号 No.	水稻品种 Rice variety	编号 No.	水稻品种 Rice variety	编号 No.	水稻品种 Rice variety
1	IRBLa-A (Pi-a)	35	绵恢146 Mianhui 146	69	泰恢808 Taihui 808	103	德香074B Dexiang 074B
2	IRBLi-F5 (Pi-i)	36	绵恢725 Mianhui 725	70	五山丝苗 Wushansimiao	104	赣香B Ganxiang B
3	IRBLks-F5 (Pi-ks)	37	南恢558 Nanhui 558	71	西科恢768 Xikehui 768	105	广8B Guang 8B
4	IRBLk-Ka (Pi-k)	38	南恢968 Nanhui 968	72	雅禾 Yahe	106	泸香618B Luxiang 618B
5	IRBLkp-K60 (Pi-kp)	39	蜀恢257 Shuhui 257	73	雅恢2115 Yahui 2115	107	绵5B Mian 5B
6	IRBLkh-K3 (Pi-kh)	40	蜀恢308 Shuhui 308	74	雅恢2116 Yahui 2116	108	忠香B Zhongxiang B
7	IRBLz-Fu (Pi-z)	41	蜀恢451 Shuhui 451	75	雅恢2117 Yahui 2117	109	内香6B Neixiang 6B
8	IRBLz5-CA (Pi-z5)	42	蜀恢498 Shuhui 498	76	雅恢2118 Yahui 2118	110	千乡654B Qianxiang 654B
9	IRBLzt-T (Pi-zt)	43	蜀恢505 Shuhui 505	77	雅恢2119 Yahui 2119	111	荃9311B Quan 9311B
10	IRBLta-K1 (Pi-ta)	44	蜀恢548 Shuhui 548	78	雅恢2199 Yahui 2199	112	蓉18B Rong 18B
11	IRBLb-B (Pi-b)	45	蜀恢573 Shuhui 573	79	雅恢2275 Yahui 2275	113	蓉7B Rong 7B
12	IRBLt-K59 (Pi-t)	46	蜀恢586 Shuhui 586	80	雅恢2348 Yahui 2348	114	双1B Shuang 1B
13	IRBLsh-B (Pi-sh)	47	蜀恢589 Shuhui 589	81	雅恢2816 Yahui 2816	115	泰丰B Taifeng B
14	IRBL1-CL (Pi-1)	48	猪毛糯 Zhumaonuo	82	雅恢2918 Yahui 2918	116	武金4B Wujin 4B
15	IRBL3-CP4 (Pi-3)	49	HR2168	83	雅恢5049 Yahui 5049	117	武香B Wuxiang B
16	IRBL5-M (Pi-5)	50	R900	84	雅恢5217 Yahui 5217	118	雅1B Ya 1B
17	IRBL7-M (Pi-7)	51	R9311	85	雨恢38 Yuhui 38	119	雅3B Ya 3B
18	IRBL9-W (Pi-9)	52	成恢19 Chenghui 19	86	岳恢9113 Yuehui 9113	120	雅7B Ya 7B
19	IRBL12-M (Pi-12)	53	乐恢188 Lehui 188	87	粤农丝苗 Yuenongsimiao	121	宜香1B Yixiang 1B
20	IRBL19-M (Pi-19)	54	乐恢584 Lehui 584	88	D297B	122	泰国香稻 Thai fragrant rice
21	IRBLkm-Ts (Pi-km)	55	泸恢17 Luhui 17	89	D35B	123	楚粳28 Chujing 28
22	IRBL20-IR24 (Pi-20)	56	泸恢8258 Luhui 8258	90	D62B	124	野香B Yexiang B
23	IRBLta2-Pi (Pi-ta2)	57	绵恢523 Mianhui 523	91	D79B	125	绵恢6139 Mianhui 6139
24	IRBL11-Zh (Pi-11)	58	绵恢528 Mianhui 528	92	F-32B	126	绵恢313 Mianhui 313
25	E33	59	绵恢662 Mianhui 662	93	F59B	127	绵恢357 Mianhui 357
26	成恢3203 Chenghui 3203	60	明恢63 Minghui 63	94	G46B	128	绵恢365 Mianhui 365
27	成恢727 Chenghui 727	61	明恢86 Minghui 86	95	川106B Chuan 106B	129	绵恢821 Mianhui 821
28	成恢993 Chenghui 993	62	内恢99-14 Neihui 99-14	96	川608B Chuan 608B	130	绵恢823 Mianhui 823
29	德恢6099 Dehui 6099	63	蜀恢137 Shuhui 137	97	川谷B Chuangu B	131	绵恢837 Mianhui 837
30	多恢1号 Duohui 1	64	蜀恢208 Shuhui 208	98	川华B Chuanhua B	132	绵恢857 Mianhui 857
31	航恢1179 Hanghui 1179	65	蜀恢527 Shuhui 527	99	川农1B Chuannong 1B	133	绵恢815 Mianhui 815
32	航恢570 Hanghui 570	66	蜀恢538 Shuhui 538	100	川农4B Chuannong 4B	134	日本晴 NPB
33	华占 Huazhan	67	蜀恢569 Shuhui 569	101	川种3B Chuanzhong 3B	135	中花11 Zhonghua 11
34	乐恢433 Lehui 433	68	蜀恢882 Shuhui 882	102	德66B De 66B	136	丽江新团黑谷 LTH

序号 No.	R基因 R gene	引物名称 Primer	引物序列 Sequence	PCR产物长度 Size of PCR production(bp)	PCR退火温度 Annealing temperature(℃)	PCR延伸时间 Extension time (s)
1	Pit	Pit-pF	TTGGAGCTACGGTTGTTCAGA	655	59	40
		Pit-pR	AGTGCTAGAACCTACGGATTCC	655	59	40
		Pit-mF	GAACCAACCGGCAACAAAaT	682	56	50
		Pit-mR	CAACTTTGATGGCAAGAGGAAcA	682	56	50
2	Pish	Pish/Pi37-F	CCTTGAGAAGCTATGTGTATACAAC	349	55	30
2	Pish	Pish/Pi35-R	AGAGAGAAACCCTGGAACTTT	349	55	30
3	Pib	Pib-F2	CCATTCCAGCAATCAGGTAGC	638	55	40
		Pib-R2	AGAGGGCCATACATTTCGCA	638	55	40
		Pib-mF	CTTTCTCTACGAGGATGCTAaC	205	60	30
		Pib-mR	GACACCTCTGCATTAAACTAaG	205	60	30
4	Pid3	Pid3-F2	TCCTTCTGTTGGTGTGCCAG	692	66	40
		Pid3-R2	TCCGTCGGACTCTATCCACA	692	66	40
		Pid3-mF	AGCTGTGGCAAGCCTAACtC	166	57	20
		Pid3-mR	GTCACAAATCATTCGCTCTTTTTaC	166	57	20
5	Pi5	Pi5-1-gF	CAATCCAATATGATAGGACTTAAAA	496	59	30
		Pi5-1-gR	GAAGGAGTTATTCTCCGCAT	496	59	30
		Pi5-1-2F	CATATATCAATTCAGCTGTGCAT	163	59	20
		Pi5-1-2R	GGCCCAACTATGGGTATAATAC	163	59	20
6	Pia	Pia(RGA5)-F	GGTGGACAGCGTGGAGATAA	280	59	20
6	Pia	Pia(RGA5)-R	CACATGGTTGAGCAAGGGTT	280	59	20
7	Pi54	Pi54-F	CAAGAATGGCAAAACTTCAAGAG	314	59	20
		Pi54-R	GAGATTATCAACTCCACTTATCTCTG	314	59	20
		Pi54-mF	CAAGAATGGCAAAACTTCAAGtG	250	60	20
		Pi54-mR2	GCCTTTTCACATGCTCTCtT	250	60	20
8	Ptr/Pi-ta2	Ptr/Pi-ta2-F	CAGCAAGGATGCAGATTTGACT	186 (R) 198 (S)	59	20
		Ptr/Pi-ta2-R	GGGTATTTCGCAGGCATAAACTC	186 (R) 198 (S)	59	20
		Ptr/Pi-ta2-F	CAGCAAGGATGCAGATTTGACT	154	58	20
		ptr-Rs	TGGTTTTGGTTYTTCTGGTTTTT	154	58	20

序号 No.	R基因 R gene	引物名称 Primer	引物序列 Sequence	PCR产物长度 Size of PCR production(bp)	PCR退火温度 Annealing temperature(℃)	PCR延伸时间 Extension time (s)
1	Pit	Pit-pF	TTGGAGCTACGGTTGTTCAGA	655	59	40
		Pit-pR	AGTGCTAGAACCTACGGATTCC	655	59	40
		Pit-mF	GAACCAACCGGCAACAAAaT	682	56	50
		Pit-mR	CAACTTTGATGGCAAGAGGAAcA	682	56	50
2	Pish	Pish/Pi37-F	CCTTGAGAAGCTATGTGTATACAAC	349	55	30
2	Pish	Pish/Pi35-R	AGAGAGAAACCCTGGAACTTT	349	55	30
3	Pib	Pib-F2	CCATTCCAGCAATCAGGTAGC	638	55	40
		Pib-R2	AGAGGGCCATACATTTCGCA	638	55	40
		Pib-mF	CTTTCTCTACGAGGATGCTAaC	205	60	30
		Pib-mR	GACACCTCTGCATTAAACTAaG	205	60	30
4	Pid3	Pid3-F2	TCCTTCTGTTGGTGTGCCAG	692	66	40
		Pid3-R2	TCCGTCGGACTCTATCCACA	692	66	40
		Pid3-mF	AGCTGTGGCAAGCCTAACtC	166	57	20
		Pid3-mR	GTCACAAATCATTCGCTCTTTTTaC	166	57	20
5	Pi5	Pi5-1-gF	CAATCCAATATGATAGGACTTAAAA	496	59	30
		Pi5-1-gR	GAAGGAGTTATTCTCCGCAT	496	59	30
		Pi5-1-2F	CATATATCAATTCAGCTGTGCAT	163	59	20
		Pi5-1-2R	GGCCCAACTATGGGTATAATAC	163	59	20
6	Pia	Pia(RGA5)-F	GGTGGACAGCGTGGAGATAA	280	59	20
6	Pia	Pia(RGA5)-R	CACATGGTTGAGCAAGGGTT	280	59	20
7	Pi54	Pi54-F	CAAGAATGGCAAAACTTCAAGAG	314	59	20
		Pi54-R	GAGATTATCAACTCCACTTATCTCTG	314	59	20
		Pi54-mF	CAAGAATGGCAAAACTTCAAGtG	250	60	20
		Pi54-mR2	GCCTTTTCACATGCTCTCtT	250	60	20
8	Ptr/Pi-ta2	Ptr/Pi-ta2-F	CAGCAAGGATGCAGATTTGACT	186 (R) 198 (S)	59	20
		Ptr/Pi-ta2-R	GGGTATTTCGCAGGCATAAACTC	186 (R) 198 (S)	59	20
		Ptr/Pi-ta2-F	CAGCAAGGATGCAGATTTGACT	154	58	20
		ptr-Rs	TGGTTTTGGTTYTTCTGGTTTTT	154	58	20

含有Pish基因的材料 Materials containing Pish	含有Pib基因的材料 Materials containing Pib	含有Pib基因的材料 Materials containing Pib	含有Pid3基因的材料 Materials containing Pid3	含有Pi5-1基因的材料 Materials containing Pi5-1
IRBLsh-B	IRBLb-B	雅恢2275 Yahui 2275	德恢6099 Dehui 6099	IRBL5-M
R900	德恢6099 Dehui 6099	雅恢2348 Yahui 2348	航恢1179 Hanghui 1179	E33
D35B	航恢570 Hanghui 570	雅恢2918 Yahui 2918	华占 Huazhan	成恢3203 Chenghui 3203
G46B	华占 Huazhan	岳恢9113 Yuehui 9113	蜀恢505 Shuhui 505	成恢727 Chenghui 727
川谷B Chuangu B	南恢558 Nanhui 558	粤农丝苗 Yuenongsimiao	蜀恢548 Shuhui 548	成恢993 Chenghui 993
川华B Chuanhua B	南恢968 Nanhui 968	川106B Chuan 106B	蜀恢586 Shuhui 586	德恢6099 Dehui 6099
川农4B Chuannong 4B	蜀恢505 Shuhui 505	川608B Chuan 608B	五山丝苗 Wushansimiao	航恢1179 Hanghui 1179
德66B De66B	蜀恢586 Shuhui 586	川华B Chuanhua B	雅禾 Yahe	航恢570 Hanghui 570
广8B Guang 8B	R900	川种3B Chuanzhong 3B	雅恢2115 Yahui 2115	华占 Huazhan
泸香618B Luxiang 618B	乐恢188 Lehui 188	泸香618B Luxiang 618B	雅恢2116 Yahui 2116	绵恢146 Mianhui 146
蓉7B Rong 7B	乐恢584 Lehui 584	忠香B Zhongxiang B	雅恢2118 Yahui 2118	南恢558 Nanhui 558
武金4B Wujin 4B	泸恢17 Luhui 17	泰丰B Taifeng B	雅恢2119 Yahui 2119	南恢968 Nanhui 968
武香B Wuxiang B	绵恢528 Mianhui 528	雅1B Ya 1B	雅恢2918 Yahui 2918	蜀恢257 Shuhui 257
雅3B Ya 3B	蜀恢569 Shuhui 569	宜香1B Yixiang 1B	雅恢5049 Yahui 5049	蜀恢308 Shuhui 308
雅7B Ya 7B	蜀恢882 Shuhui 882	楚粳28 Chugeng 28	雅恢5217 Yahui 5217	蜀恢451 Shuhui 451
含有Pish基因	五山丝苗 Wushansimiao	野香B Yexiang B	雨恢38 Yuhui 38	蜀恢498 Shuhui 498
	西科恢768 Xikehui 768	绵恢313 Mianhui 313	蓉7B Rong 7B	蜀恢548 Shuhui 548
	雅恢2115 Yahui 2115	绵恢365 Mianhui 365	双1B Shuang 1B	蜀恢589 Shuhui 589
	雅恢2116 Yahui 2116	绵恢821 Mianhui 821	雅1B Ya 1B	HR2168
	雅恢2118 Yahui 2118	绵恢823 Mianhui 823	绵恢815 Mianhui 815	R900
	雅恢2119 Yahui 2119	绵恢815 Mianhui 815		乐恢584 Lehui 584
	雅恢2199 Yahui 2199			泸恢8258 Luhui 8258
				绵恢523 Mianhui 523
				绵恢528 Mianhui 528
				广8B Guang 8B
				绵恢6139 Mianhui 6139
				绵恢313 Mianhui 313
				绵恢821 Mianhui 821
				绵恢837 Mianhui 837
				绵恢857 Mianhui 857

水稻中八个稻瘟病抗性基因特异分子标记的开发及应用

Development and Application of Specific Molecular Markers for Eight Rice Blast Resistance Genes in Rice

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