中国水稻科学 ›› 2024, Vol. 38 ›› Issue (3): 277-289.DOI: 10.16819/j.1001-7216.2024.231110
收稿日期:
2023-11-15
修回日期:
2024-01-22
出版日期:
2024-05-10
发布日期:
2024-05-13
通讯作者:
*email:cuikehui@mail.hzau.edu.cn
基金资助:
ZHAO Yiting, XIE Keran, GAO Ti, CUI Kehui*()
Received:
2023-11-15
Revised:
2024-01-22
Online:
2024-05-10
Published:
2024-05-13
Contact:
*email:cuikehui@mail.hzau.edu.cn
摘要:
【目的】明确分蘖期干旱锻炼对幼穗分化期高温下水稻穗发育和产量形成的影响,并探究其生理机理。【方法】以两优培九(热敏感型)和汕优63(耐热型)为材料,在盆栽条件下设置全生育期淹灌和分蘖期干旱锻炼、幼穗分化期适温和高温共四种水分和温度处理组合,研究干旱锻炼对高温胁迫下水稻颖花分化与退化、花粉活力、颖花育性、颖花大小、产量及穗生理特性的影响。【结果】与淹灌相比,在适温下干旱锻炼对两个品种产量无显著影响,但高温下干旱锻炼后两优培九和汕优63的产量分别显著提高了54.0%和20.1%,这主要是因为结实率和千粒重显著提高。与淹灌相比,幼穗分化期高温下,分蘖期干旱锻炼显著增强两优培九颖花过氧化物酶和超氧化物歧化酶活性,降低了丙二醛含量,其花粉活力和颖花育性分别显著提高了26.0%和39.0%。与适温相比,全生育期淹灌下,高温显著降低了两个品种的颖花大小和总颖花分化数。与淹灌相比,分蘖期干旱锻炼显著提高了幼穗分化期高温下两品种穗非结构性碳水化合物含量和颖花细胞分裂素(反式玉米素+反式玉米素核苷)含量,两个品种颖花长、颖花宽和总颖花分化数平均显著增加了3.2%、4.4%和15.0%。【结论】分蘖期干旱锻炼可通过增加抗氧化酶活性、非结构性碳水化合物和细胞分裂素含量来促进颖花发育和提高颖花育性,进而缓解幼穗分化期高温对水稻产量的不利影响,研究结果可为高温热害频发地区的水稻生产实践提供理论指导。
赵艺婷, 谢可冉, 高逖, 崔克辉. 水稻分蘖期干旱锻炼对幼穗分化期高温下穗发育和产量形成的影响[J]. 中国水稻科学, 2024, 38(3): 277-289.
ZHAO Yiting, XIE Keran, GAO Ti, CUI Kehui. Effects of Drought Priming During Tillering Stage on Panicle Development and Yield Formation Under High Temperature During Panicle Initiation Stage in Rice[J]. Chinese Journal OF Rice Science, 2024, 38(3): 277-289.
图1 干旱锻炼处理期间的土壤水势数据为均值 ± 标准误 (n=3)。LYPJ:两优培九;SY63:汕优63。W1:淹灌;W2:干旱锻炼。下同。
Fig. 1. Soil water potential during drought priming treatment Data are shown as mean ± SE (n=3). LYPJ, Liangyoupeijiu; SY63, Shanyou 63. W1, Flooding irrigation; W2, Drought priming. The same as below.
图2 高温处理期间温室的平均温度和湿度数值为高温处理期间各时间点的均值 ± 标准误 (n=4)。T1:适温处理;T2:高温处理。
Fig. 2. Average daytime temperature and relative humidity in the greenhouse during high temperature treatment The data at each point-in-time are the average values over the duration of the high temperature treatment ± SE (n=4). T1, Normal temperature; T2, High temperature.
品种 Variety | 处理 Treatment | 产量 Yield(g/plant) | 单株穗数 Panicles per plant | 每穗粒数 Grains per panicle | 结实率 Seed setting rate(%) | 千粒重 1000-grain weight(g) |
---|---|---|---|---|---|---|
两优培九 | W1T1 | 24.6±0.1 a | 11.4±0.6 a | 133.7±2.8 a | 69.0±1.7 a | 23.6±0.1 a |
Liangyoupeijiu | W2T1 | 24.4±1.0 a | 11.9±0.5 a | 133.2±2.0 a | 64.7±2.1 a | 23.9±0.3 a |
W1T2 | 15.5±1.1 b | 11.0±0.2 a | 131.6±5.6 a | 48.5±3.1 b | 22.3±0.2 b | |
W2T2 | 23.9±1.6 a | 12.0±0.7 a | 131.9±11.6 a | 66.2±1.8 a | 23.3±0.3 a | |
汕优63 | W1T1 | 35.5±0.4 a* | 12.3±0.4 a | 133.9±5.8 ab | 84.6±3.5 a* | 25.6±0.1 a* |
Shanyou 63 | W2T1 | 34.6±1.9 a* | 13.3±0.2 a | 142.5±8.3 a | 73.4±1.5 b* | 25.2±0.1 a* |
W1T2 | 25.9±1.2 b* | 12.3±0.4 a | 119.7±3.1 b | 78.7±3.5 ab* | 22.6±0.1 c | |
W2T2 | 31.2±1.2 a* | 13.4±0.6 a | 128.1±2.3 ab | 78.4±1.4 ab* | 23.5±0.2 b | |
ANOVA | ||||||
品种 Variety(V) | ++ | ns | ns | ++ | ++ | |
温度 Temperature(T) | ++ | ns | ns | ns | ++ | |
水分 Water content(W) | + | ++ | ns | ns | + | |
V×T | ns | ns | ns | ns | ++ | |
V×W | ns | ns | ns | ++ | ns | |
T×W | ++ | ns | ns | ++ | + | |
V×T×W | ns | ns | ns | ns | ns |
表1 干旱锻炼对幼穗分化期高温下水稻产量的影响
Table 1. Effects of drought priming on rice yield under high temperature during panicle initiation stage
品种 Variety | 处理 Treatment | 产量 Yield(g/plant) | 单株穗数 Panicles per plant | 每穗粒数 Grains per panicle | 结实率 Seed setting rate(%) | 千粒重 1000-grain weight(g) |
---|---|---|---|---|---|---|
两优培九 | W1T1 | 24.6±0.1 a | 11.4±0.6 a | 133.7±2.8 a | 69.0±1.7 a | 23.6±0.1 a |
Liangyoupeijiu | W2T1 | 24.4±1.0 a | 11.9±0.5 a | 133.2±2.0 a | 64.7±2.1 a | 23.9±0.3 a |
W1T2 | 15.5±1.1 b | 11.0±0.2 a | 131.6±5.6 a | 48.5±3.1 b | 22.3±0.2 b | |
W2T2 | 23.9±1.6 a | 12.0±0.7 a | 131.9±11.6 a | 66.2±1.8 a | 23.3±0.3 a | |
汕优63 | W1T1 | 35.5±0.4 a* | 12.3±0.4 a | 133.9±5.8 ab | 84.6±3.5 a* | 25.6±0.1 a* |
Shanyou 63 | W2T1 | 34.6±1.9 a* | 13.3±0.2 a | 142.5±8.3 a | 73.4±1.5 b* | 25.2±0.1 a* |
W1T2 | 25.9±1.2 b* | 12.3±0.4 a | 119.7±3.1 b | 78.7±3.5 ab* | 22.6±0.1 c | |
W2T2 | 31.2±1.2 a* | 13.4±0.6 a | 128.1±2.3 ab | 78.4±1.4 ab* | 23.5±0.2 b | |
ANOVA | ||||||
品种 Variety(V) | ++ | ns | ns | ++ | ++ | |
温度 Temperature(T) | ++ | ns | ns | ns | ++ | |
水分 Water content(W) | + | ++ | ns | ns | + | |
V×T | ns | ns | ns | ns | ++ | |
V×W | ns | ns | ns | ++ | ns | |
T×W | ++ | ns | ns | ++ | + | |
V×T×W | ns | ns | ns | ns | ns |
图3 干旱锻炼对幼穗分化期高温下水稻花粉活力和颖花育性的影响数据为均值 ± 标准误 (n=3)。不同小写字母表示同一品种在不同水分和温度处理组合下同一性状在P < 0.05水平下差异显著(LSD法)。*表示相同水分与温度处理组合下两个品种间同一性状在P < 0.05水平下差异显著。V表示品种,T表示温度,W表示水分。+和++分别表示在P < 0.05和P < 0.01水平下差异显著,ns表示在P < 0.05水平下差异不显著。W1T1表示淹灌和适温,W2T1表示干旱锻炼和适温,W1T2表示淹灌和高温,W2T2表示干旱锻炼和高温。
Fig. 3. Effects of drought priming on rice pollen viability and spikelet fertility under high temperature during panicle initiation stage Data are shown as mean ± SE (n=3). Different lowercase letters indicate significant difference for a given trait among the four water and temperature treatment combinations for the same variety at P<0.05 (LSD test). * indicates the significant difference for a given trait between two varieties in the same combination of water and temperature treatment at P<0.05. V indicates variety, T indicates temperature, and W indicates water. + and ++ indicate significant difference at P <0.05 and P <0.01, respectively, while ns indicates no significant difference at P <0.05. W1T1 indicates flooded irrigation and normal temperature, W2T1 indicates drought priming and normal temperature, W1T2 indicates flooded irrigation and high temperature, and W2T2 indicates drought priming and high temperature.
品种 Variety | 处理 Treatment | 抽穗期Heading stage | 成熟期Mature stage | |||
---|---|---|---|---|---|---|
颖花长 Spikelet length(mm) | 颖花宽 Spikelet width(mm) | 籽粒长 Grain length(mm) | 籽粒宽 Grain width(mm) | |||
两优培九 | W1T1 | 9.07±0.02 a* | 3.00±0.03 a | 8.85±0.05 a* | 2.62±0.05 b | |
Liangyoupeijiu | W2T1 | 8.97±0.00 a* | 2.98±0.04 a | 8.82±0.02 ab* | 2.69±0.02 a | |
W1T2 | 8.77±0.07 b* | 2.82±0.02 b | 8.72±0.03 b* | 2.54±0.03 c | ||
W2T2 | 9.00±0.13 a* | 2.96±0.07 a | 8.80±0.08 ab* | 2.63±0.02 b | ||
汕优63 | W1T1 | 8.40±0.11 a | 3.28±0.04 a* | 8.44±0.06 a | 2.96±0.00 a* | |
Shanyou 63 | W2T1 | 8.37±0.02 a | 3.22±0.01 a* | 8.48±0.04 a | 2.94±0.02 a* | |
W1T2 | 7.98±0.01 b | 2.97±0.02 c* | 8.15±0.07 c | 2.78±0.01 c* | ||
W2T2 | 8.28±0.03 a | 3.09±0.01 b* | 8.32±0.09 b | 2.87±0.01 b* | ||
ANOVA | ||||||
品种 Variety(V) | ++ | ++ | ++ | ++ | ||
温度 Temperature(T) | + | ++ | + | + | ||
水分 Water content(W) | ns | ns | ns | ++ | ||
V×T | ns | ns | ns | ns | ||
V×W | ns | ns | ns | + | ||
T×W | + | + | ns | ++ | ||
V×T×W | ns | ns | ns | + |
表2 干旱锻炼对幼穗分化期高温下颖花大小和籽粒大小的影响
Table 2. Effects of drought priming on rice spikelet size and grain size under high temperature during panicle initiation stage
品种 Variety | 处理 Treatment | 抽穗期Heading stage | 成熟期Mature stage | |||
---|---|---|---|---|---|---|
颖花长 Spikelet length(mm) | 颖花宽 Spikelet width(mm) | 籽粒长 Grain length(mm) | 籽粒宽 Grain width(mm) | |||
两优培九 | W1T1 | 9.07±0.02 a* | 3.00±0.03 a | 8.85±0.05 a* | 2.62±0.05 b | |
Liangyoupeijiu | W2T1 | 8.97±0.00 a* | 2.98±0.04 a | 8.82±0.02 ab* | 2.69±0.02 a | |
W1T2 | 8.77±0.07 b* | 2.82±0.02 b | 8.72±0.03 b* | 2.54±0.03 c | ||
W2T2 | 9.00±0.13 a* | 2.96±0.07 a | 8.80±0.08 ab* | 2.63±0.02 b | ||
汕优63 | W1T1 | 8.40±0.11 a | 3.28±0.04 a* | 8.44±0.06 a | 2.96±0.00 a* | |
Shanyou 63 | W2T1 | 8.37±0.02 a | 3.22±0.01 a* | 8.48±0.04 a | 2.94±0.02 a* | |
W1T2 | 7.98±0.01 b | 2.97±0.02 c* | 8.15±0.07 c | 2.78±0.01 c* | ||
W2T2 | 8.28±0.03 a | 3.09±0.01 b* | 8.32±0.09 b | 2.87±0.01 b* | ||
ANOVA | ||||||
品种 Variety(V) | ++ | ++ | ++ | ++ | ||
温度 Temperature(T) | + | ++ | + | + | ||
水分 Water content(W) | ns | ns | ns | ++ | ||
V×T | ns | ns | ns | ns | ||
V×W | ns | ns | ns | + | ||
T×W | + | + | ns | ++ | ||
V×T×W | ns | ns | ns | + |
品种 Variety | 处理 Treatment | 总枝梗分化数 NBD (No./panicle) | 一次枝梗分化数 NPBD (No./panicle) | 二次枝梗分化数 NSBD (No./panicle) | 一次枝梗退化数 NPBG (No./panicle) | 二次枝梗退化数 NSBG (No./panicle) |
---|---|---|---|---|---|---|
两优培九 | W1T1 | 73.7±2.3 a | 12.8±0.4 a | 60.9±1.9 a | 0.0±0.0 a | 22.3±2.3 ab |
Liangyoupeijiu | W2T1 | 68.0±1.6 a | 12.3±0.2 a | 55.7±1.5 a | 0.0±0.0 a | 18.9±1.6 ab |
W1T2 | 66.8±1.5 a | 12.1±0.1 a | 54.7±1.4 a | 0.0±0.0 a | 23.8±1.7 a | |
W2T2 | 68.0±1.9 a | 12.2±0.1 a | 55.8±1.8 a | 0.0±0.0 a | 18.6±1.1 b | |
汕优63 | W1T1 | 67.3±0.7 a | 14.0±0.3 a | 53.3±1.0 a* | 0.0±0.0 a | 17.7±1.2 a |
Shanyou 63 | W2T1 | 67.0±2.1 a | 13.2±0.7 a | 53.8±1.5 a | 0.0±0.0 a | 17.2±3.6 a |
W1T2 | 64.4±0.2 a | 13.4±0.1 a* | 51.0±0.3 a | 0.0±0.0 a | 22.4±1.3 a | |
W2T2 | 65.8±1.0 a | 13.1±0.1 a* | 52.7±1.1 a | 0.0±0.0 a | 17.9±0.9 a | |
ANOVA | ||||||
品种 Variety(V) | ns | ns | + | ns | ns | |
温度 Temperature(T) | + | ns | + | ns | ns | |
水分 Water content(W) | ns | ns | ns | ns | ns | |
V×T | ns | ns | ns | ns | ns | |
V×W | ns | ns | ns | ns | ns | |
T×W | ns | ns | ns | ns | ns | |
V×T×W | ns | ns | ns | ns | ns |
表3 干旱锻炼对幼穗分化期高温下每穗枝梗数的影响
Table 3. Effects of drought priming on the number of rachis branches per panicle under high temperature during panicle initiation stage
品种 Variety | 处理 Treatment | 总枝梗分化数 NBD (No./panicle) | 一次枝梗分化数 NPBD (No./panicle) | 二次枝梗分化数 NSBD (No./panicle) | 一次枝梗退化数 NPBG (No./panicle) | 二次枝梗退化数 NSBG (No./panicle) |
---|---|---|---|---|---|---|
两优培九 | W1T1 | 73.7±2.3 a | 12.8±0.4 a | 60.9±1.9 a | 0.0±0.0 a | 22.3±2.3 ab |
Liangyoupeijiu | W2T1 | 68.0±1.6 a | 12.3±0.2 a | 55.7±1.5 a | 0.0±0.0 a | 18.9±1.6 ab |
W1T2 | 66.8±1.5 a | 12.1±0.1 a | 54.7±1.4 a | 0.0±0.0 a | 23.8±1.7 a | |
W2T2 | 68.0±1.9 a | 12.2±0.1 a | 55.8±1.8 a | 0.0±0.0 a | 18.6±1.1 b | |
汕优63 | W1T1 | 67.3±0.7 a | 14.0±0.3 a | 53.3±1.0 a* | 0.0±0.0 a | 17.7±1.2 a |
Shanyou 63 | W2T1 | 67.0±2.1 a | 13.2±0.7 a | 53.8±1.5 a | 0.0±0.0 a | 17.2±3.6 a |
W1T2 | 64.4±0.2 a | 13.4±0.1 a* | 51.0±0.3 a | 0.0±0.0 a | 22.4±1.3 a | |
W2T2 | 65.8±1.0 a | 13.1±0.1 a* | 52.7±1.1 a | 0.0±0.0 a | 17.9±0.9 a | |
ANOVA | ||||||
品种 Variety(V) | ns | ns | + | ns | ns | |
温度 Temperature(T) | + | ns | + | ns | ns | |
水分 Water content(W) | ns | ns | ns | ns | ns | |
V×T | ns | ns | ns | ns | ns | |
V×W | ns | ns | ns | ns | ns | |
T×W | ns | ns | ns | ns | ns | |
V×T×W | ns | ns | ns | ns | ns |
品种 Variety | 处理 Treatment | 总颖花分化数 NSD (No./panicle) | 一次颖花分化数 NPSD (No./panicle) | 二次颖花分化数 NSSD (No./panicle) | 一次颖花退化数 NPSG (No./panicle) | 二次颖花退化数 NSSG (No./panicle) |
---|---|---|---|---|---|---|
两优培九 | W1T1 | 189.4±5.5 a | 69.6±2.9 a | 119.9±4.1 a | 0.0±0.0 a | 7.4±0.9 b* |
Liangyoupeijiu | W2T1 | 187.7±1.3 a | 69.6±1.7 a | 118.1±2.1 a | 0.0±0.0 a | 7.6±3.9 b |
W1T2 | 162.3±1.9 b | 66.0±0.8 a | 96.3±2.2 b* | 0.1±0.1 a | 16.4±3.5 a* | |
W2T2 | 182.9±3.2 a | 67.0±1.3 a | 115.9±2.5 a | 0.1±0.1 a | 8.0±1.8 b | |
汕优63 | W1T1 | 197.0±5.3 a | 80.7±3.3 a | 116.3±7.3 a | 0.1±0.1 a | 3.0±1.0 b |
Shanyou 63 | W2T1 | 193.2±0.8 a | 80.6±3.6 a | 112.7±3.8 a | 0.0±0.0 a | 3.4±0.7 b |
W1T2 | 160.3±3.7 b | 74.0±2.0 a* | 86.3±2.8 b | 0.3±0.3 a | 8.0±1.0 a | |
W2T2 | 188.2±4.0 a | 75.3±1.8 a* | 112.9±5.1 a | 0.0±0.0 a | 4.7±0.9 ab | |
ANOVA | ||||||
品种 Variety (V) | ns | ns | + | ns | ns | |
温度 Temperature (T) | ++ | ns | ++ | ns | + | |
水分 Water content (W) | ++ | ns | ++ | ns | ns | |
V×T | ns | ns | ns | ns | ns | |
V×W | ns | ns | ns | ns | ns | |
T×W | ++ | ns | ++ | ns | ns | |
V×T×W | ns | ns | ns | ns | ns |
表4 干旱锻炼对幼穗分化期高温下每穗颖花数的影响
Table 4. Effects of drought priming on spikelets per panicle under high temperature during panicle initiation stage
品种 Variety | 处理 Treatment | 总颖花分化数 NSD (No./panicle) | 一次颖花分化数 NPSD (No./panicle) | 二次颖花分化数 NSSD (No./panicle) | 一次颖花退化数 NPSG (No./panicle) | 二次颖花退化数 NSSG (No./panicle) |
---|---|---|---|---|---|---|
两优培九 | W1T1 | 189.4±5.5 a | 69.6±2.9 a | 119.9±4.1 a | 0.0±0.0 a | 7.4±0.9 b* |
Liangyoupeijiu | W2T1 | 187.7±1.3 a | 69.6±1.7 a | 118.1±2.1 a | 0.0±0.0 a | 7.6±3.9 b |
W1T2 | 162.3±1.9 b | 66.0±0.8 a | 96.3±2.2 b* | 0.1±0.1 a | 16.4±3.5 a* | |
W2T2 | 182.9±3.2 a | 67.0±1.3 a | 115.9±2.5 a | 0.1±0.1 a | 8.0±1.8 b | |
汕优63 | W1T1 | 197.0±5.3 a | 80.7±3.3 a | 116.3±7.3 a | 0.1±0.1 a | 3.0±1.0 b |
Shanyou 63 | W2T1 | 193.2±0.8 a | 80.6±3.6 a | 112.7±3.8 a | 0.0±0.0 a | 3.4±0.7 b |
W1T2 | 160.3±3.7 b | 74.0±2.0 a* | 86.3±2.8 b | 0.3±0.3 a | 8.0±1.0 a | |
W2T2 | 188.2±4.0 a | 75.3±1.8 a* | 112.9±5.1 a | 0.0±0.0 a | 4.7±0.9 ab | |
ANOVA | ||||||
品种 Variety (V) | ns | ns | + | ns | ns | |
温度 Temperature (T) | ++ | ns | ++ | ns | + | |
水分 Water content (W) | ++ | ns | ++ | ns | ns | |
V×T | ns | ns | ns | ns | ns | |
V×W | ns | ns | ns | ns | ns | |
T×W | ++ | ns | ++ | ns | ns | |
V×T×W | ns | ns | ns | ns | ns |
图4 干旱锻炼对幼穗分化期高温下穗部非结构性碳水化合物含量的影响数据为均值±标准误(n=3)。不同小写字母表示同一品种在不同水分和温度处理组合下同一性状在P < 0.05水平下差异显著(LSD法)。*表示相同水分与温度处理组合下两个品种间同一性状在P < 0.05水平下差异显著。V表示品种,T表示温度,W表示水分。+表示在P < 0.05水平下差异显著,ns表示在P < 0.05水平下差异不显著。W1T1表示淹灌和适温,W2T1表示干旱锻炼和适温,W1T2表示淹灌和高温,W2T2表示干旱锻炼和高温。
Fig. 4. Effects of drought priming on non-structural carbohydrate contents in panicle under high temperature during panicle initiation stage Data are shown as mean ± SE (n=3). Different lowercase letters indicate significant difference for a given trait among the four water and temperature treatment combinations for the same variety at P<0.05 (LSD test). * indicates significant difference for a given trait between two varieties in the same combination of water and temperature treatment at P<0.05. V indicates variety, T indicates temperature, and W indicates water. + indicates significant difference at P <0.05, and ns indicates no significant difference at P <0.05. W1T1 indicates flooded irrigation and normal temperature, W2T1 indicates drought priming and normal temperature, W1T2 indicates flooded irrigation and high temperature, and W2T2 indicates drought priming and high temperature.
图5 干旱锻炼对幼穗分化期高温下颖花丙二醛含量和抗氧化酶活性的影响数据为均值 ± 标准误 (n=3)。不同小写字母表示同一品种在不同水分和温度处理组合下同一性状在P < 0.05水平下差异显著(LSD法)。*表示相同水分与温度处理组合下两个品种间同一性状在P < 0.05水平下差异显著。V表示品种,T表示温度,W表示水分。+和++分别表示在P < 0.05和P < 0.01水平下差异显著,ns表示在P < 0.05水平下差异不显著。W1T1表示淹灌和适温,W2T1表示干旱锻炼和适温,W1T2表示淹灌和高温,W2T2表示干旱锻炼和高温。
Fig. 5. Effects of drought priming on MDA content and antioxidant enzyme activities in spikelet under high temperature during panicle initiation stage Data are shown as mean ± SE (n=3). Different lowercase letters indicate significant difference for a given trait among the four water and temperature treatment combinations for the same variety at P<0.05 (LSD test). * indicates significant difference for a given trait between two varieties in the same combination of water and temperature treatment at P<0.05. V indicates variety, T indicates temperature, and W indicates water. + and ++ indicate significant difference at P <0.05 and P <0.01, respectively, while ns indicates no significant difference at P <0.05. W1T1 indicates flooded irrigation and normal temperature, W2T1 indicates drought priming and normal temperature, W1T2 indicates flooded irrigation and high temperature, and W2T2 indicates drought priming and high temperature.
品种 Variety | 处理 Treatment | 活性细胞 分裂素总量 aCTKs(ng/g) | 反式玉米素含量 tZ(ng/g) | 反式玉米素 核苷含量 tZR(ng/g) | 异戊烯腺嘌呤含量 iP(ng/g) | 异戊烯腺苷含量 iPA(ng/g) | 异戊烯腺苷 磷酸含量 iPMP(ng/g) |
---|---|---|---|---|---|---|---|
两优培九 | W1T1 | 19.38±0.97 a | 0.70±0.01 a | 1.41±0.08 a | 1.90±0.09 a | 1.32±0.06 a* | 14.05±1.11 a |
Liangyoupeijiu | W2T1 | 19.54±1.34 a | 0.69±0.02 a | 1.55±0.09 a | 1.74±0.07 a* | 1.23±0.06 a* | 14.33±1.16 a |
W1T2 | 15.05±0.53 c | 0.11±0.00 c | 0.69±0.02 b | 2.01±0.06 a* | 1.35±0.08 a | 10.89±0.44 b | |
W2T2 | 17.18±0.95 b | 0.15±0.00 b | 1.41±0.06 a | 1.89±0.08 a | 1.33±0.11 a | 12.40±0.82 ab | |
汕优63 | W1T1 | 19.43±0.73 a | 0.64±0.06 a | 1.71±0.14 a* | 1.70±0.21 a | 1.17±0.08 a | 14.23±0.27 a |
Shanyou 63 | W2T1 | 19.56±0.97 a | 0.65±0.07 a | 1.69±0.11 a | 1.58±0.07 a | 1.09±0.08 a | 14.54±0.77 a |
W1T2 | 18.59±0.65 a* | 0.64±0.02 a* | 1.36±0.03 b* | 1.80±0.06 a | 1.29±0.05 a | 13.50±0.63 a* | |
W2T2 | 19.29±1.12 a* | 0.71±0.03 a* | 1.65±0.07 a* | 1.80±0.01 a | 1.30±0.10 a | 13.84±1.03 a | |
ANOVA | |||||||
品种 Variety(V) | + | ++ | ns | + | ns | ns | |
温度 Temperature(T) | + | ++ | ++ | ns | ns | ns | |
水分 Water content(W) | ns | ns | ++ | ns | ns | ns | |
V×T | ns | ++ | ns | ns | ns | ns | |
V×W | ns | ns | ++ | ns | ns | ns | |
T×W | ns | ns | ++ | ns | ns | ns | |
V×T×W | ns | ns | ns | ns | ns | ns |
表5 干旱锻炼对幼穗分化期高温下颖花细胞分裂素含量的影响
Table 5. Effects of drought priming on cytokinin contents in spikelet under high temperature during panicle initiation stage
品种 Variety | 处理 Treatment | 活性细胞 分裂素总量 aCTKs(ng/g) | 反式玉米素含量 tZ(ng/g) | 反式玉米素 核苷含量 tZR(ng/g) | 异戊烯腺嘌呤含量 iP(ng/g) | 异戊烯腺苷含量 iPA(ng/g) | 异戊烯腺苷 磷酸含量 iPMP(ng/g) |
---|---|---|---|---|---|---|---|
两优培九 | W1T1 | 19.38±0.97 a | 0.70±0.01 a | 1.41±0.08 a | 1.90±0.09 a | 1.32±0.06 a* | 14.05±1.11 a |
Liangyoupeijiu | W2T1 | 19.54±1.34 a | 0.69±0.02 a | 1.55±0.09 a | 1.74±0.07 a* | 1.23±0.06 a* | 14.33±1.16 a |
W1T2 | 15.05±0.53 c | 0.11±0.00 c | 0.69±0.02 b | 2.01±0.06 a* | 1.35±0.08 a | 10.89±0.44 b | |
W2T2 | 17.18±0.95 b | 0.15±0.00 b | 1.41±0.06 a | 1.89±0.08 a | 1.33±0.11 a | 12.40±0.82 ab | |
汕优63 | W1T1 | 19.43±0.73 a | 0.64±0.06 a | 1.71±0.14 a* | 1.70±0.21 a | 1.17±0.08 a | 14.23±0.27 a |
Shanyou 63 | W2T1 | 19.56±0.97 a | 0.65±0.07 a | 1.69±0.11 a | 1.58±0.07 a | 1.09±0.08 a | 14.54±0.77 a |
W1T2 | 18.59±0.65 a* | 0.64±0.02 a* | 1.36±0.03 b* | 1.80±0.06 a | 1.29±0.05 a | 13.50±0.63 a* | |
W2T2 | 19.29±1.12 a* | 0.71±0.03 a* | 1.65±0.07 a* | 1.80±0.01 a | 1.30±0.10 a | 13.84±1.03 a | |
ANOVA | |||||||
品种 Variety(V) | + | ++ | ns | + | ns | ns | |
温度 Temperature(T) | + | ++ | ++ | ns | ns | ns | |
水分 Water content(W) | ns | ns | ++ | ns | ns | ns | |
V×T | ns | ++ | ns | ns | ns | ns | |
V×W | ns | ns | ++ | ns | ns | ns | |
T×W | ns | ns | ++ | ns | ns | ns | |
V×T×W | ns | ns | ns | ns | ns | ns |
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