水稻东野型不育系异交不亲和性的鉴定和遗传分析

doi:10.16819/j.1001-7216.2020.0610

中国水稻科学 ›› 2020, Vol. 34 ›› Issue (6): 520-524.DOI: 10.16819/j.1001-7216.2020.0610

水稻东野型不育系异交不亲和性的鉴定和遗传分析

熊涛^1,^2,^#, 聂元元^1,^#, 毛方明¹, 雷建国¹, 毛凌华¹, 朱珊³, 黄仁良³, 沈显华^3,^*(), 严松^1,^3,^*()

¹ 江西省农业科学院江西省超级水稻研究发展中心南昌 330200
² 江西省农业科学院科技管理处南昌 330200
³ 江西省农业科学院水稻研究所南昌 330200

收稿日期:2020-06-30 修回日期:2020-09-29 出版日期:2020-11-10 发布日期:2020-11-10
通讯作者: 熊涛,聂元元,沈显华,严松
基金资助:
江西省重点研发计划资助项目(20181ACF60001);江西省自然科学基金资助项目(20202BABL205015)

Identification and Genetic Analysis of Cross-incompatibility ina RiceDW-type Sterile Line

Tao XIONG^1,^2,^#, Yuanyuan NIE^1,^#, Fangming MAO¹, Jianguo LEI¹, Linghua MAO¹, Shan ZHU³, Renliang HUANG³, Xianhua SHEN^3,^*(), Song YAN^1,^3,^*()

¹ Jiangxi Super-rice Research and Development Center, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
² Department of Science and Technology Management, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
³ Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received:2020-06-30 Revised:2020-09-29 Online:2020-11-10 Published:2020-11-10
Contact: Tao XIONG, Yuanyuan NIE, Xianhua SHEN, Song YAN

摘要/Abstract

摘要： 目的在东野型新三系杂交水稻研究过程中,发现不育系与部分品种杂交或回交存在异交不亲和性。对该性状开展研究,为东野型不育系异交不亲和性的改良提供理论依据,促进东野型三系体系的应用。方法采用东野型同质异核不育系和野败型异质同核不育系分别与保持系品种进行杂交,研究东野型不育系异交不亲和性的产生原因,进而利用一套染色体单片段代换系分别与东野型不育系DY1A杂交,开展遗传分析,并进行基因初步定位。结果异交不亲和性由核质互作产生,且有两种类型,分别发生在杂交当代和回交世代。针对第一种类型,初步遗传分析显示异交不亲和性在细胞核内受单隐性基因控制,暂命名为CI₁_(t),将该基因初步定位在第8染色体RM6838和RM5767两个标记之间,与RM3395连锁。结论东野型不育系的异交不亲和性可以通过分子手段进行遗传改良。

关键词: 东野型不育系, 异交不亲和性, 染色体单片段代换系, 遗传分析, 基因定位

Abstract:

【Objective】In the process of breeding DW-type three-line hybrid rice, cross incompatibility (CI) was found when the cytoplasmic male sterile (CMS) lines were crossed/backcrossed with some varieties. We focused on the traittolayatheoretical basis for improving CI of DW-type CMS lines, so as to promote the application ofDW-type three-line. 【Method】With isocytoplasmallonuclear materials of DW-type and isonuclear alloplasmic male sterile lines of WA-type as female parents, maintainer lines as male parents, cross experimentswere carried out to investigate the cause of CI. Genetic analysis and gene rough mapping were conducted using a set of chromosome single segment substitution lines, which were crossed to a DW-type CMS line DY1A, respectively.【Result】The CI was caused by nuclear-cytoplasmic interactions. Two types of CI were observed that the first type occurred in F₀ crosses and another after backcrossing. For the first type, primarily genetic analysis showed that the CI was controlled by a recessive gene in the nucleus, temporarily named CI₁_(t), which was then mapped between the two markers RM6838 and RM5767, linked toRM3395,on chromosome 8.【Conclusion】The CI of DW-type CMS linescould beimprovedby molecular methods.

Key words: DW-type sterile line, cross incompatibility, chromosome single segment substitution lines, genetic analysis, gene mapping

中图分类号:

S511.032

熊涛, 聂元元, 毛方明, 雷建国, 毛凌华, 朱珊, 黄仁良, 沈显华, 严松. 水稻东野型不育系异交不亲和性的鉴定和遗传分析[J]. 中国水稻科学, 2020, 34(6): 520-524.

Tao XIONG, Yuanyuan NIE, Fangming MAO, Jianguo LEI, Linghua MAO, Shan ZHU, Renliang HUANG, Xianhua SHEN, Song YAN. Identification and Genetic Analysis of Cross-incompatibility ina RiceDW-type Sterile Line[J]. Chinese Journal OF Rice Science, 2020, 34(6): 520-524.

图/表 3

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轮回父本 Recurrent male parent	各世代结实率 Seed setting rates in different generations /%
轮回父本 Recurrent male parent	F₀	BC₁F₀	BC₂F₀	BC₃F₀
粳型japonica
Sasanishiki	1.5±0.8	1.1±0.7	/	/
02428	77.0±5.2	13.5±6.5	0	/
秀水123Xiushui 123	79.4±4.5	16.7±5.6	0	/
籼型indica
华占Huazhan	84.2±6.8	39.3±4.4	13.5±7.2	0
93-11	83.3±5.1	21.7±2.6	1.3±0.7	/
广恢308Guanghui 308	88.6±3.9	35.0±6.8	16.8±5.7	0
金23BJin23B	85.5±5.5	22.2±4.2	5.3±3.6	4.5±2.1
五丰BWufeng B	83.0±7.2	36.3±11.0	17.7±8.2	2.2±0.9
Habataki	82.5±8.0	78.7±6.6	85.0±4.2	82.8±5.0
R668	80.7±4.9	79.5±4.5	82.3±4.5	81.0±4.8
Ⅱ-32B	81.6±5.6	80.0±5.4	84.5±6.7	82.1±5.5

轮回父本 Recurrent male parent	各世代结实率 Seed setting rates in different generations /%
轮回父本 Recurrent male parent	F₀	BC₁F₀	BC₂F₀	BC₃F₀
粳型japonica
Sasanishiki	1.5±0.8	1.1±0.7	/	/
02428	77.0±5.2	13.5±6.5	0	/
秀水123Xiushui 123	79.4±4.5	16.7±5.6	0	/
籼型indica
华占Huazhan	84.2±6.8	39.3±4.4	13.5±7.2	0
93-11	83.3±5.1	21.7±2.6	1.3±0.7	/
广恢308Guanghui 308	88.6±3.9	35.0±6.8	16.8±5.7	0
金23BJin23B	85.5±5.5	22.2±4.2	5.3±3.6	4.5±2.1
五丰BWufeng B	83.0±7.2	36.3±11.0	17.7±8.2	2.2±0.9
Habataki	82.5±8.0	78.7±6.6	85.0±4.2	82.8±5.0
R668	80.7±4.9	79.5±4.5	82.3±4.5	81.0±4.8
Ⅱ-32B	81.6±5.6	80.0±5.4	84.5±6.7	82.1±5.5

水稻东野型不育系异交不亲和性的鉴定和遗传分析

Identification and Genetic Analysis of Cross-incompatibility ina RiceDW-type Sterile Line

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