Wxmp基因背景下可溶性淀粉合成酶基因SSⅡa和去分支酶基因PUL对水稻蒸煮食味品质的影响

doi:10.16819/j.1001-7216.2020.9120

中国水稻科学 ›› 2020, Vol. 34 ›› Issue (3): 217-227.DOI: 10.16819/j.1001-7216.2020.9120

Wx^mp基因背景下可溶性淀粉合成酶基因SSⅡa和去分支酶基因PUL对水稻蒸煮食味品质的影响

姚姝¹, 张亚东¹, 刘燕清¹, 赵春芳¹, 周丽慧¹, 陈涛¹, 赵庆勇¹, 朱镇¹, Balakrishna PILLAY^2,^*, 王才林^1,^*()

¹江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心,南京 210014
²夸祖鲁-纳塔尔大学（西维尔校区）农业工程科学院生命科学系,南非德班

收稿日期:2019-11-24 修回日期:2020-02-05 出版日期:2020-05-15 发布日期:2020-05-10
通讯作者: Balakrishna PILLAY,王才林
基金资助:
江苏省自然科学基金资助项目(BK20180302);江苏省农业科技自主创新基金资助项目(CX[18]1001);江苏省重点研发计划资助项目(BE2018357);现代农业产业技术体系建设专项资金资助项目(CARS-1-62);江苏省作物基因组学和分子育种重点实验室开放课题(PL201902)

Allelic Effects on Eating and Cooking Quality of SSⅡa and PUL Genes Under Wx^mp Background in Rice

Shu YAO¹, Yadong ZHANG¹, Yanqing LIU¹, Chunfang ZHAO¹, Lihui ZHOU¹, Tao CHEN¹, Qingyong ZHAO¹, Zhen ZHU¹, PILLAY Balakrishna^2,^*, Cailin WANG^1,^*()

¹Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu High Quality Rice Research and Development Center, Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China
²School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Durban, South Africa

Received:2019-11-24 Revised:2020-02-05 Online:2020-05-15 Published:2020-05-10
Contact: PILLAY Balakrishna, Cailin WANG

摘要/Abstract

摘要：

【目的】分析在同一主效基因（Wx^mp）背景下可溶性淀粉合成酶基因SSⅡa和去分支酶基因PUL对稻米蒸煮食味品质的影响,以期为水稻品质遗传改良提供依据。【方法】选择在SSⅡa和PUL存在多态性而其他淀粉合成酶相关基因没有多态性的半糯品系宁0145和粳稻品种武运粳21进行杂交,获得F₂群体与F₃株系。利用分子标记,选择含有Wx^mp基因的F₂单株与F₃株系,将这些F₂单株与F₃株系分成SSⅡaⁿPULⁿ、SSⅡaⁿPUL^w、SSⅡa^wPULⁿ和SSⅡa^wPUL^w4种基因型（ⁿ和^w分别表示该基因来源于宁0145和武运粳21）,分析不同基因型蒸煮食味品质性状的差异,探讨同一Wx^mp基因背景下不同SSⅡa和PUL等位基因对蒸煮食味品质性状的影响。【结果】不同基因型间蒸煮食味品质性状均存在显著差异,来源于武运粳21的SSⅡa^w基因和PUL^w基因分别使直链淀粉含量增加0.29%~1.00%和0.62%~1.18%,且PUL的效应大于SSⅡa,两者间存在互作效应。SSⅡa^w基因和PUL^w基因降低胶稠度和崩解值,提高了热浆黏度、冷胶黏度、消减值和回复值,对糊化温度、峰值黏度和峰值时间的作用较小。【结论】明确了Wx^mp背景下SSⅡa和PUL基因对稻米蒸煮食味品质的遗传效应,该研究结果为SSⅡa和PUL基因的分子标记辅助选择改良稻米品质提供了理论依据。

关键词: 半糯基因, 蒸煮食味品质, 可溶性淀粉合成酶基因, 极限糊精酶基因, 等位基因效应

Abstract:

【Objective】Our aim is to analyze the genetic effects of soluble starch synthase gene SSⅡa and debranching enzyme gene PUL on eating and cooking quality under the same main gene background of Wx^mp, so as to lay a theoretical basis for rice quality improvement. 【Method】In this study, a semi-glutinous rice line Ning 0145 and japonica rice variety Wuyunjing 21 were crossed to obtain F₂ population and F₃ lines. There was polymorphism in soluble starch synthase gene SSⅡa and debranching enzyme gene PUL but no polymorphism in other starch synthase related genes between the two parents. With molecular markers, some F₂ plants and F₃ lines containing Wx^mp gene were selected and divided into four genotypes, SSⅡaⁿPULⁿ, SSⅡaⁿPUL^w, SSⅡa^wPULⁿand SSⅡa^wPUL^w(ⁿ and ^w indicated that the genes were contributed by Ning 0145 and Wuyunjing 21, respectively). The allelic effects of SSⅡa and PUL genes on eating and cooking quality under the same Wx^mp gene background was investigated by analyzing the eating and cooking quality and its differences among different genotypes. 【Result】There were significant differences for eating and cooking quality among genotypes of different parental origins. The allelic gene SSⅡa^w and PUL^w from Wuyunjing 21 increased amylose content by 0.29%-1.00% and 0.62%-1.18% respectively, and the effect of PUL was greater than that of SSⅡa. There was interaction between SSⅡa and PUL genes. The SSⅡa^w and PUL^w genes also decreased gel consistency and breakdown viscosity, increased hot paste viscosity, cool paste viscosity, setback viscosity and consistency viscosity, but had little effect on gelatinization temperature, peak viscosity and peak time. 【Conclusion】The genetic effects of SSⅡa and PUL genes on cooking and eating quality of rice under the background of Wx^mp gene were clarified. The results lay a theoretical basis for improving rice quality by molecular marker-assisted selection of SSⅡa and PUL genes.

Key words: semi-glutinous gene, eating and cooking quality, soluble starch synthase gene, pullulanase gene, allelic effect

中图分类号:

姚姝, 张亚东, 刘燕清, 赵春芳, 周丽慧, 陈涛, 赵庆勇, 朱镇, Balakrishna PILLAY, 王才林. Wx^mp基因背景下可溶性淀粉合成酶基因SSⅡa和去分支酶基因PUL对水稻蒸煮食味品质的影响[J]. 中国水稻科学, 2020, 34(3): 217-227.

Shu YAO, Yadong ZHANG, Yanqing LIU, Chunfang ZHAO, Lihui ZHOU, Tao CHEN, Qingyong ZHAO, Zhen ZHU, PILLAY Balakrishna, Cailin WANG. Allelic Effects on Eating and Cooking Quality of SSⅡa and PUL Genes Under Wx^mp Background in Rice[J]. Chinese Journal OF Rice Science, 2020, 34(3): 217-227.

图/表 10

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基因 Gene	引物序列 Primer sequence(5′-3′)	产物大小 Product size/bp	退火温度 Annealing temperature/℃
Wx^mp	O-F: ATGTTGTGTTCTTGTGTTCTTTGCAGGC	439/200	65
	O-R: GTAGATCTTCTCACCGGTCTTTCCCCAA	439/200	65
	I-F: GGGTGAGGTTTTTCCATTGCTACAATCG	439/292	65
	I-R: GTCGATGAACACACGGTCGACTCAAT	439/292	65
SSIIa(Y12)	F: CCAATACCGTAAACTAGCGACTATG	90/81	55
SSIIa(Y12)	R: TACAGGTAGAATGGCAGTGGTG	90/81	55
PUL(Y38)	F: AGTTCGCTAGTCATCTGCTCG	198/194	55
PUL(Y38)	R: CCACATGTCCTTGTCTCCACTT	198/194	55

基因 Gene	引物序列 Primer sequence(5′-3′)	产物大小 Product size/bp	退火温度 Annealing temperature/℃
Wx^mp	O-F: ATGTTGTGTTCTTGTGTTCTTTGCAGGC	439/200	65
	O-R: GTAGATCTTCTCACCGGTCTTTCCCCAA	439/200	65
	I-F: GGGTGAGGTTTTTCCATTGCTACAATCG	439/292	65
	I-R: GTCGATGAACACACGGTCGACTCAAT	439/292	65
SSIIa(Y12)	F: CCAATACCGTAAACTAGCGACTATG	90/81	55
SSIIa(Y12)	R: TACAGGTAGAATGGCAGTGGTG	90/81	55
PUL(Y38)	F: AGTTCGCTAGTCATCTGCTCG	198/194	55
PUL(Y38)	R: CCACATGTCCTTGTCTCCACTT	198/194	55

变异来源 Source of variation	自由度 df	平方和 SS	均方 MS	F值 F value
重复 Repeat	1	0.11	0.11	0.23
F₂单株间 Among F₂ plants	30	61.20	2.04	5.22^**
基因型间 Among genotypes	3	50.64	16.88	35.54^**
基因型内 Within genotypes	27	10.56	0.39	0.82
误差 Error	30	14.25	0.48
总变异 Total variation	61	75.56

变异来源 Source of variation	自由度 df	平方和 SS	均方 MS	F值 F value
重复 Repeat	1	0.11	0.11	0.23
F₂单株间 Among F₂ plants	30	61.20	2.04	5.22^**
基因型间 Among genotypes	3	50.64	16.88	35.54^**
基因型内 Within genotypes	27	10.56	0.39	0.82
误差 Error	30	14.25	0.48
总变异 Total variation	61	75.56

参数 Parameter	直链淀粉含量AC/%	胶稠度GC/mm	糊化温度GT/℃	峰值黏度PV/cP	热浆黏度HPV/cP	崩解值BDV/cP	冷胶黏度CPV/cP	消减值SBV/cP	回复值CSV/cP
最小值 Min	9.12	28.00	70.00	2445	1086	1109	1712	-1214	626
最大值 Max	12.69	103.50	76.10	4124	2312	2092	3356	11	1403
平均 Mean	10.42	53.55	72.84	3366	1834	1532	2831	-535	997
标准差 SD	0.72	16.61	1.15	312.17	228.52	219.69	282.26	306.53	153.78
变异系数 CV/%	6.92	31.02	1.58	9.27	12.46	14.34	9.97	-57.31	15.42

Wx^mp基因背景下可溶性淀粉合成酶基因SSⅡa和去分支酶基因PUL对水稻蒸煮食味品质的影响

Allelic Effects on Eating and Cooking Quality of SSⅡa and PUL Genes Under Wx^mp Background in Rice

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