黑龙江省稻稗对丁草胺的抗性测定及交互抗性的研究

doi:10.16819/j.1001-7216.2020.8143

摘要/Abstract

摘要：

【目的】明确黑龙江省水田稻稗(Echinochloa oryzicola Vasing)对丁草胺的抗药性水平以及对其他细胞分裂抑制剂的交互抗药性水平。【方法】采用琼脂法和整株盆栽法测定了黑龙江省14个地区53个田块的稻稗对丁草胺的抗药性以及对其他3种细胞分裂抑制剂丙草胺、苯噻酰草胺和莎稗磷的交互抗性。【结果】琼脂法检测出庆安03、东京城镇02等4个稻稗生物型对丁草胺产生了中等水平抗性,抗性指数为12.92~26.03,占供试稻稗生物型的7.55%;兴隆镇03、阿城04等4个稻稗生物型对丁草胺产生了低等水平抗性,抗性指数为6.63~8.86,占供试稻稗生物型的7.55%;庆安01、汤原01等5个稻稗生物型对丁草胺敏感性下降,抗性指数为3.18~4.84,占供试稻稗生物型的9.43%;其余阿城02、尚志02等40个稻稗生物型对丁草胺表现敏感,抗性指数均小于3,占供试稻稗生物型的75.47%。选取上述测定结果当中抗性最高的两个生物型,使用整株盆栽法进行对丁草胺的抗性水平验证。发现庆安03和东京城镇02同样产生了中等水平抗性,抗性指数分别为16.78和13.27;同时测定两个抗性生物型对丙草胺、苯噻酰草胺和莎稗磷3种细胞分裂抑制剂的交互抗性,结果显示,庆安03稻稗生物型对丁草胺和丙草胺产生了交互抗性;东京城镇02稻稗生物型对丁草胺、丙草胺和莎稗磷产生了交互抗性。【结论】黑龙江省部分地区稻稗对丁草胺产生了抗药性,且对丙草胺和莎稗磷产生了交互抗性。

关键词: 稻稗, 丁草胺, 细胞分裂抑制剂, 抗性测定, 交互抗性

Abstract:

：【Objcetive】In order to identify the resistance of Echinochloa oryzicola Vasing in Heilongjiang Province to butachlor and the cross-resistance to other cell division inhibitors, 【Mehtod】the agar culture and the whole-plant pot-culture method were used to determine the resistance level of Echinochloa oryzicola Vasing populations collected from 53 plots in 14 areas of Heilongjiang Province to butachlor and the cross-resistance to other three cell-division inhibitors (pretilachlor, mefenacet and anilofos).【Result】The resistant level of Echinochloa oryzicola Vasing to butachlor detected by the agar culture method showed that four populations including Qingan 03 and Dongjingchengzhen 02 have developed a medium level of resistance with resistance indexes ranging from 6.63 to 8.86, accounting for 7.55% of total populations. Four populations, Xinglongzhen 04 and Acheng 04 included, have developed a low level of resistance with resistance indexes between 12.92 and 26.03, accounting for 7.55% of total populations. Five populations such as Qingan 01 and Tangyuan 01 are on the downward trend in sensitivity, with resistance indexes between 3.18 and 4.84, accounting for 9.43% of total populations. Other forty populations such as Acheng 02 are sensitive to butachlor, with resistance indexes less than 3, accounting for 75.47% of total populations. The two biotypes with the highest resistance among the above determinations were selected for the determination of the resistance to butachlor in the whole plant pot culture experiment. It was verified that Qingan 03 and Dongjingchengzheng 02 have also developed a medium level of resistance, with resistance indexes of 16.78 and 13.27, respectively. Meanwhile, the cross-resistance of the two resistant biotypes to pretilachlor, mefenacet and anilofos was tested. Qingan 03 has developed cross-resistance to butachlor and pretilachlor, Dongjingchengzhen 02 has developed cross-resistance to butachlor, pretilachlor and anilofos.【Conclusion】Echinochloa oryzicola Vasing in some areas of Heilongjiang Province have developed resistance to butachlor and cross resistance to pretilachlor and anilofos.

Key words: Echinochloa oryzicola Vasing, butachlor, cell-division inhibitors, resistance determination, cross-resistance

中图分类号:

刘亚光, 吴绘鹏, 李敏, 朱金文, 张苏新, 张春鹏. 黑龙江省稻稗对丁草胺的抗性测定及交互抗性的研究[J]. 中国水稻科学, 2020, 34(1): 88-94.

Yaguang LIU, Huipeng WU, Min LI, Jinwen ZHU, Suxin ZHANG, Chunpeng ZHANG. Determination of Resistance to Butachlor and Its Cross-resistance in Echinochloa oryzicola Vasing in Heilongjiang Province[J]. Chinese Journal OF Rice Science, 2020, 34(1): 88-94.

图/表 4

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用量 Dose
丙草胺（g/hm²）	121.875	243.75	487.5	975	1950
苯噻酰草胺（g/hm²）	131.25	262.5	525	1050	2100
莎稗磷（g/hm²）	73.125	146.25	292.5	585	1170

用量 Dose
丙草胺（g/hm²）	121.875	243.75	487.5	975	1950
苯噻酰草胺（g/hm²）	131.25	262.5	525	1050	2100
莎稗磷（g/hm²）	73.125	146.25	292.5	585	1170

序号 Number	生物型 Biol types	回归方程(Y=bx+a) Regression formula	相关系数(R²) Correlation coefficient	IC₅₀ /(mL·L^-1)	95%置信区间 95 % confidence interval of IC₅₀	抗性指数 Resistant index (RI)
1	庆安03 Qingan 03	Y=0.6249x+5.3495	0.9977	0.2759	0.2333–0.3262	26.03
2	东京城镇02 Dongjingchengzhen 02	Y=0.7161x+5.4681	0.9881	0.2220	0.1532–0.3216	20.94
3	东京城镇01 Dongjingchengzhen 01	Y=0.8097x+5.6677	0.9509	0.1497	0.0719–0.3120	14.12
4	东京城镇04 Dongjingchengzhen 04	Y=0.5719x+5.4937	0.9949	0.1370	0.1092–0.1719	12.92
5	兴隆镇03 Xinglongzhen 03	Y=0.6036x+5.6202	0.9964	0.0939	0.0777–0.1133	8.86
6	阿城04 Acheng 04	Y=0.5032x+5.5592	0.9745	0.0774	0.0462–0.1297	7.30
7	五常01 Wuchang 01	Y=0.6321x+5.7243	0.9940	0.0715	0.0558–0.0915	6.75
8	五常04 Wuchang 04	Y=0.7573x+5.8731	0.9962	0.0703	0.0577–0.0858	6.63
9	庆安01 Qingan 01	Y=0.7769x+6.0019	0.9980	0.0513	0.0442–0.0597	4.84
10	汤原01 Tangyuan 01	Y=0.4799x+5.6407	0.9916	0.0462	0.0338–0.0632	4.36
11	庆安02 Qingan 02	Y=0.8115x+6.0861	0.9889	0.0459	0.0320–0.0658	4.33
12	五常02 Wuchang 02	Y=0.6557x+5.9063	0.9838	0.0415	0.0266–0.0648	3.92
13	东京城镇06 Dongjingchengzhen 06	Y=0.5756x+5.8474	0.9970	0.0337	0.0276–0.0412	3.18
14	上集镇02 Shangjizhen 02	Y=0.5422x+5.8246	0.9929	0.0301	0.0220–0.0413	2.84
15	兴隆镇04 Xinglongzhen 04	Y=0.6402x+5.9935	0.9921	0.0281	0.0200–0.0393	2.65
16	兴隆镇02 Xinglongzhen 02	Y=0.5921x+5.922	0.9891	0.0277	0.0186–0.0413	2.61
17	阿城03 Acheng 03	Y=0.6678x+6.0432	0.9945	0.0274	0.0207–0.0363	2.58
18	海林04 Hailin 04	Y=0.6681x+6.0488	0.9968	0.0269	0.0217–0.0335	2.54
19	五常03 Wuchang 03	Y=0.4909x+5.7742	0.9687	0.0265	0.0132–0.0529	2.50
20	汤原03 Tangyuan 03	Y=0.4187x+5.6701	0.9895	0.0251	0.0168–0.0374	2.37
21	东京城镇03 Dongjingchengzhen 03	Y=0.6216x+5.9989	0.9664	0.0247	0.0119–0.0514	2.33
22	庆安04 Qingan 04	Y=0.6298x+6.0237	0.9915	0.0237	0.0165–0.0341	2.24
23	汤原02 Tangyuan 02	Y=0.3882x+5.6343	0.9988	0.0232	0.0203–0.0266	2.19
24	穆棱01 Muleng 01	Y=0.5035x+5.8225	0.9931	0.0232	0.0167–0.0323	2.19
25	东京城镇07 Dongjingchengzhen 07	Y=0.4979x+5.8154	0.9940	0.0230	0.0169–0.0313	2.17
26	友谊农场03 Youyinongchang 03	Y=0.5335x+5.8759	0.9965	0.0228	0.0180–0.0289	2.15
27	穆棱04 Muleng 04	Y=0.4784x+5.787	0.9925	0.0226	0.0160–0.0320	2.13
28	上集镇03 Shangjizhen 03	Y=0.4824x+5.8069	0.9978	0.0213	0.0176–0.0257	2.01
29	853农场01 853 nongchang 01	Y=0.5656x+5.951	0.9989	0.0208	0.0182–0.0238	1.96
30	兴凯湖01 Xingkaihu 01	Y=0.5636x+5.9508	0.9900	0.0206	0.0137–0.0310	1.94
31	穆棱02 Muleng 02	Y=0.5192x+5.8805	0.9985	0.0201	0.0172–0.0236	1.90
32	佳木斯02 Jiamusi 02	Y=0.6503x+6.107	0.9961	0.0198	0.0153–0.0257	1.87
33	853农场02 853 nongchang 02	Y=0.646x+6.1073	0.9892	0.0193	0.0125–0.0298	1.82
34	穆棱05 Muleng 05	Y=0.4825x+5.8287	0.9959	0.0192	0.0147–0.0250	1.81
35	尚志01 Shangzhi 01	Y=0.5203x+5.8939	0.9913	0.0191	0.0130–0.0283	1.80
36	阿城01 Acheng 01	Y=0.4779x+5.8312	0.9889	0.0182	0.0117–0.0285	1.72
37	友谊农场02 Youyinongchang 02	Y=0.6096x+6.0661	0.9740	0.0178	0.0089–0.0357	1.68
38	海林03 Hailin 03	Y=0.5763x+6.0181	0.9898	0.0171	0.0111–0.0264	1.61
39	海林02 Hailin 02	Y=0.5845x+6.0352	0.9712	0.0169	0.0081–0.0355	1.59
40	友谊农场01 Youyinongchang 01	Y=0.4714x+5.8371	0.9928	0.0168	0.0116–0.0242	1.58
41	佳木斯01 Jiamusi 01	Y=0.5176x+5.9181	0.9957	0.0168	0.0127–0.0223	1.58
42	东京城镇05 Dongjingchengzhen 05	Y=0.4935x+5.8794	0.9961	0.0165	0.0126–0.0217	1.56
43	五常05 Wuchang 05	Y=0.4696x+5.8397	0.9860	0.0163	0.0097–0.0273	1.54
44	上集镇04 Shangjizhen 04	Y=0.6508x+6.1747	0.9790	0.0157	0.0082–0.0298	1.48
45	853农场03 853 nongchang 03	Y=0.7087x+6.2852	0.9982	0.0154	0.0127–0.0185	1.45
46	上集镇01 Shangjizhen 01	Y=0.4217x+5.7735	0.9782	0.0146	0.0075–0.0285	1.38
47	兴隆镇01 Xinglongzhen 01	Y=0.4694x+5.8867	0.9875	0.0129	0.0077–0.0216	1.22
48	友谊农场05 Youyinongchang 05	Y=0.436x+5.8249	0.9800	0.0128	0.0066–0.0248	1.21
49	兴凯湖02 Xingkaihu 02	Y=0.4115x+5.7806	0.9885	0.0127	0.0077–0.0208	1.20
50	穆棱03 Muleng 03	Y=0.4815x+5.9147	0.9874	0.0126	0.0075–0.0212	1.19
51	海林01 Hailin 01	Y=0.495x+5.948	0.9930	0.0122	0.0082–0.0180	1.15
52	尚志02 Shangzhi 02	Y=0.4834x+5.9385	0.9808	0.0114	0.0059–0.0222	1.08
53	阿城02 Acheng 02	Y=0.293x+5.578	0.9872	0.0106	0.0061–0.0184	1.00

序号 Number	生物型 Biol types	回归方程(Y=bx+a) Regression formula	相关系数(R²) Correlation coefficient	IC₅₀ /(mL·L^-1)	95%置信区间 95 % confidence interval of IC₅₀	抗性指数 Resistant index (RI)
1	庆安03 Qingan 03	Y=0.6249x+5.3495	0.9977	0.2759	0.2333–0.3262	26.03
2	东京城镇02 Dongjingchengzhen 02	Y=0.7161x+5.4681	0.9881	0.2220	0.1532–0.3216	20.94
3	东京城镇01 Dongjingchengzhen 01	Y=0.8097x+5.6677	0.9509	0.1497	0.0719–0.3120	14.12
4	东京城镇04 Dongjingchengzhen 04	Y=0.5719x+5.4937	0.9949	0.1370	0.1092–0.1719	12.92
5	兴隆镇03 Xinglongzhen 03	Y=0.6036x+5.6202	0.9964	0.0939	0.0777–0.1133	8.86
6	阿城04 Acheng 04	Y=0.5032x+5.5592	0.9745	0.0774	0.0462–0.1297	7.30
7	五常01 Wuchang 01	Y=0.6321x+5.7243	0.9940	0.0715	0.0558–0.0915	6.75
8	五常04 Wuchang 04	Y=0.7573x+5.8731	0.9962	0.0703	0.0577–0.0858	6.63
9	庆安01 Qingan 01	Y=0.7769x+6.0019	0.9980	0.0513	0.0442–0.0597	4.84
10	汤原01 Tangyuan 01	Y=0.4799x+5.6407	0.9916	0.0462	0.0338–0.0632	4.36
11	庆安02 Qingan 02	Y=0.8115x+6.0861	0.9889	0.0459	0.0320–0.0658	4.33
12	五常02 Wuchang 02	Y=0.6557x+5.9063	0.9838	0.0415	0.0266–0.0648	3.92
13	东京城镇06 Dongjingchengzhen 06	Y=0.5756x+5.8474	0.9970	0.0337	0.0276–0.0412	3.18
14	上集镇02 Shangjizhen 02	Y=0.5422x+5.8246	0.9929	0.0301	0.0220–0.0413	2.84
15	兴隆镇04 Xinglongzhen 04	Y=0.6402x+5.9935	0.9921	0.0281	0.0200–0.0393	2.65
16	兴隆镇02 Xinglongzhen 02	Y=0.5921x+5.922	0.9891	0.0277	0.0186–0.0413	2.61
17	阿城03 Acheng 03	Y=0.6678x+6.0432	0.9945	0.0274	0.0207–0.0363	2.58
18	海林04 Hailin 04	Y=0.6681x+6.0488	0.9968	0.0269	0.0217–0.0335	2.54
19	五常03 Wuchang 03	Y=0.4909x+5.7742	0.9687	0.0265	0.0132–0.0529	2.50
20	汤原03 Tangyuan 03	Y=0.4187x+5.6701	0.9895	0.0251	0.0168–0.0374	2.37
21	东京城镇03 Dongjingchengzhen 03	Y=0.6216x+5.9989	0.9664	0.0247	0.0119–0.0514	2.33
22	庆安04 Qingan 04	Y=0.6298x+6.0237	0.9915	0.0237	0.0165–0.0341	2.24
23	汤原02 Tangyuan 02	Y=0.3882x+5.6343	0.9988	0.0232	0.0203–0.0266	2.19
24	穆棱01 Muleng 01	Y=0.5035x+5.8225	0.9931	0.0232	0.0167–0.0323	2.19
25	东京城镇07 Dongjingchengzhen 07	Y=0.4979x+5.8154	0.9940	0.0230	0.0169–0.0313	2.17
26	友谊农场03 Youyinongchang 03	Y=0.5335x+5.8759	0.9965	0.0228	0.0180–0.0289	2.15
27	穆棱04 Muleng 04	Y=0.4784x+5.787	0.9925	0.0226	0.0160–0.0320	2.13
28	上集镇03 Shangjizhen 03	Y=0.4824x+5.8069	0.9978	0.0213	0.0176–0.0257	2.01
29	853农场01 853 nongchang 01	Y=0.5656x+5.951	0.9989	0.0208	0.0182–0.0238	1.96
30	兴凯湖01 Xingkaihu 01	Y=0.5636x+5.9508	0.9900	0.0206	0.0137–0.0310	1.94
31	穆棱02 Muleng 02	Y=0.5192x+5.8805	0.9985	0.0201	0.0172–0.0236	1.90
32	佳木斯02 Jiamusi 02	Y=0.6503x+6.107	0.9961	0.0198	0.0153–0.0257	1.87
33	853农场02 853 nongchang 02	Y=0.646x+6.1073	0.9892	0.0193	0.0125–0.0298	1.82
34	穆棱05 Muleng 05	Y=0.4825x+5.8287	0.9959	0.0192	0.0147–0.0250	1.81
35	尚志01 Shangzhi 01	Y=0.5203x+5.8939	0.9913	0.0191	0.0130–0.0283	1.80
36	阿城01 Acheng 01	Y=0.4779x+5.8312	0.9889	0.0182	0.0117–0.0285	1.72
37	友谊农场02 Youyinongchang 02	Y=0.6096x+6.0661	0.9740	0.0178	0.0089–0.0357	1.68
38	海林03 Hailin 03	Y=0.5763x+6.0181	0.9898	0.0171	0.0111–0.0264	1.61
39	海林02 Hailin 02	Y=0.5845x+6.0352	0.9712	0.0169	0.0081–0.0355	1.59
40	友谊农场01 Youyinongchang 01	Y=0.4714x+5.8371	0.9928	0.0168	0.0116–0.0242	1.58
41	佳木斯01 Jiamusi 01	Y=0.5176x+5.9181	0.9957	0.0168	0.0127–0.0223	1.58
42	东京城镇05 Dongjingchengzhen 05	Y=0.4935x+5.8794	0.9961	0.0165	0.0126–0.0217	1.56
43	五常05 Wuchang 05	Y=0.4696x+5.8397	0.9860	0.0163	0.0097–0.0273	1.54
44	上集镇04 Shangjizhen 04	Y=0.6508x+6.1747	0.9790	0.0157	0.0082–0.0298	1.48
45	853农场03 853 nongchang 03	Y=0.7087x+6.2852	0.9982	0.0154	0.0127–0.0185	1.45
46	上集镇01 Shangjizhen 01	Y=0.4217x+5.7735	0.9782	0.0146	0.0075–0.0285	1.38
47	兴隆镇01 Xinglongzhen 01	Y=0.4694x+5.8867	0.9875	0.0129	0.0077–0.0216	1.22
48	友谊农场05 Youyinongchang 05	Y=0.436x+5.8249	0.9800	0.0128	0.0066–0.0248	1.21
49	兴凯湖02 Xingkaihu 02	Y=0.4115x+5.7806	0.9885	0.0127	0.0077–0.0208	1.20
50	穆棱03 Muleng 03	Y=0.4815x+5.9147	0.9874	0.0126	0.0075–0.0212	1.19
51	海林01 Hailin 01	Y=0.495x+5.948	0.9930	0.0122	0.0082–0.0180	1.15
52	尚志02 Shangzhi 02	Y=0.4834x+5.9385	0.9808	0.0114	0.0059–0.0222	1.08
53	阿城02 Acheng 02	Y=0.293x+5.578	0.9872	0.0106	0.0061–0.0184	1.00

生物型 Biotype	回归方程 Regression formula	相关系数(R²) Correlation coefficient	ED₅₀ /(g·hm^-2)	95%置信区间 95 % confidence interval of ED₅₀	抗性指数 Resistant index(RI)
庆安03 Qingan 03	Y=0.7056x+2.4725	0.9940	3819.5309	2958.367–4931.375	16.78
东京城镇02 Dongjingchengzhen 02	Y=0.8711x+1.9682	0.9903	3021.6911	2233.694–4087.676	13.27
阿城02 Acheng 02	Y=0.7716x+3.1811	0.9879	227.6830	155.3234–333.7524	1.00