辽宁省粳稻品种稻瘟病抗性基因分析

doi:10.16819/j.1001-7216.2019.8071

摘要/Abstract

摘要：

【目的】为明确辽宁省260份粳稻品种中稻瘟病抗性基因的类型,评价抗性基因利用价值,为辽宁省水稻抗病育种提供参考。【方法】选用9个稻瘟病抗性基因的特异引物分析辽宁省已审定的260份粳稻品种抗性基因种类、基因组合类型及地域分布情况,基于抗病基因检测条带的有无进行品种聚类分析,苗期人工喷雾接种、分蘖盛期和蜡熟期田间自然发病调查,评价9个抗性单基因系的抗病性。【结果】辽宁省粳稻品种中含有抗性基因数量从0~7个不等,抗性基因Pikh检出率最高,其次是Pikm和Pi1;所有参试品种均未检测到Pi9基因,仅在辽宁省农业科学院育成的辽粳421中检测到Pi40基因。参试的水稻品种中共有62个基因组合类型。基于抗性基因检测结果进行聚类分析,可以将260份粳稻材料分为11个类群。9个抗性单基因系中,Pi40抗性最好;其次是Pi9、Pigm和Pita。【结论】抗性基因Pi40、Pi9、Pigm和Pita在辽宁省粳稻品种选育和生产上具有重要的应用潜力;参试品种的80.38%集中在第Ⅴ、Ⅵ、Ⅶ和Ⅸ类群,没有明显的地域差异;参试品种抗病基因组合类型不够丰富,亲缘关系较近。

关键词: 粳稻, 稻瘟病, 抗性基因

Abstract:

【Objective】To clarify the rice blast resistance genes in 260 japonica rice varieties in Liaoning Province, evaluate their utilization value, and provide reference for rice breeding,【Method】specific primers of nine rice blast resistance genes were selected to analyze the species, the quantity and the geographical distribution of resistance genes of 260 japonica rice varieties in Liaoning, and the cluster analysis of varieties was based on the detection bands of disease resistance genes. The disease resistance of the nine resistant mongenic rice lines was evaluated by seedling-spray inoculation, natural disease investigation during the active tillering stage and ripening stage.【Result】The number of blast resistance genes in japonica rice from Liaoning varied from 0 to 7, and the frequency of Pikh gene ranked highest, followed by the Pikm and Pi1. Pi9 gene was not detected in all tested varieties, and Pi40 gene was only detected in Liaojing 421 bred by the Liaoning Academy of Agricultural Sciences. There were 62 gene combinations in the rice varieties tested, and based on the clustering analysis of the results of the resistance gene detection, 260 japonica rice materials can be divided into 11 groups. Among the nine resistant mongenic lines, Pi40 showed the best resistance, followed by Pi9, Pigm and Pita.【Conclusion】The resistance genes Pi9, Pi40, Pita, and Pigm have important application potential in breeding in Liaoning Province. 80.38% of the tested varieties concentrate in groups V, VI, VII and IX. There is no obvious geographical difference; the types of disease resistance gene combinations of the tested varieties are relatively less abundant and the genetic relationship is relatively close.

Key words: japonica rice, rice blast, resistance gene

中图分类号:

潘争艳, 邱福林, 吕桂兰, 马秀芳, 商文奇, 白元俊, 徐正进. 辽宁省粳稻品种稻瘟病抗性基因分析[J]. 中国水稻科学, 2019, 33(3): 241-248.

Zhengyan PAN, Fulin QIU, Guilan LÜ, Xiufang MA, Wenqi SHANG, Yuanjun BAI, Zhengjin XU. Analysis of Rice Blast Resistance Genes in japonica Rice Varieties in Liaoning Province[J]. Chinese Journal OF Rice Science, 2019, 33(3): 241-248.

图/表 6

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基因 Gene	引物 Primer	引物序列 Primer sequence（5′-3′）	片段长度 Length/bp	参考文献 Reference
Pi9	Pi9SNP-F	CGCCGGTTGATAAGTAAAAGCT	126	[11]
	Pi9SNP-R	CAAGAACTAATATCTACCCATGG
Pi40	P2-F	CAACAAACGGGTCGACAAAGG	100-250	[12, 13]
	P2-R	CCCCCAGGTCGTGATACCTTC
Pigm	ZJ58.7-F	ACTTGCTGGGAGAAGGATT	236	[14, 15]
	ZJ58.7-R	AGTTCGTACTTTTCAGGCT
Piz-t	Piz-t PA-F	ATGTGGATGCTGTGTTAT	176	[11]
	Piz-t PA-R	TAGTTTGCTGCTCAATAAGTA
Pita	YL155/YL87Pita-F	AGCAGGTTATAAGCTAGGCC	1042	[16]
Pita	YL155/YL87Pita-R	CTACCAACAAGTTCATCAAA
	YL183/YL87Npita-F	AGCAGGTTATAAGCTAGCTAT	1042	[16]
	YL183/YL87Npita-R	CTACCAACAAGTTCATCAAAS
Pib	PibdomPib-F	GAACAATGCCCAAACTTGAGA	365	[17, 18]
	PibdomPib-R	GGGTCCACATGTCAGTGAGC
	Npib-F	TCGGTGCCTCGGTAGTCAGT	803	[17, 18]
	Npib-R	GGGAAGCGGATCCTAGGTCTS
Pi1	RM144-F	TGCCCTGGCGCAAATTTGATCC	234	[19]
Pi1	RM144-R	GCTAGAGGAGATCAGATGGTAGTGCATG
Pikm	Dkm1-F	CTGGAGAGCCTTCCGTGTCGAC	223	[15]
	Dkm1-R	TCTTCACGACCTCAATCCTCCC
	Dkm2-F	GTTGTTCACTCCGTATCTACTACGTC	291	[15]
	Dkm2-R	TTCCTCCGTGATCTCACCAACG
Pikh	Pikh-F	CAATCTCCAAAGTTTTCAGG	216/359	[20]
Pikh	Pikh-R	GCTTCAATCACTGCTAGACC	216/359	[20]

基因 Gene	引物 Primer	引物序列 Primer sequence（5′-3′）	片段长度 Length/bp	参考文献 Reference
Pi9	Pi9SNP-F	CGCCGGTTGATAAGTAAAAGCT	126	[11]
	Pi9SNP-R	CAAGAACTAATATCTACCCATGG
Pi40	P2-F	CAACAAACGGGTCGACAAAGG	100-250	[12, 13]
	P2-R	CCCCCAGGTCGTGATACCTTC
Pigm	ZJ58.7-F	ACTTGCTGGGAGAAGGATT	236	[14, 15]
	ZJ58.7-R	AGTTCGTACTTTTCAGGCT
Piz-t	Piz-t PA-F	ATGTGGATGCTGTGTTAT	176	[11]
	Piz-t PA-R	TAGTTTGCTGCTCAATAAGTA
Pita	YL155/YL87Pita-F	AGCAGGTTATAAGCTAGGCC	1042	[16]
Pita	YL155/YL87Pita-R	CTACCAACAAGTTCATCAAA
	YL183/YL87Npita-F	AGCAGGTTATAAGCTAGCTAT	1042	[16]
	YL183/YL87Npita-R	CTACCAACAAGTTCATCAAAS
Pib	PibdomPib-F	GAACAATGCCCAAACTTGAGA	365	[17, 18]
	PibdomPib-R	GGGTCCACATGTCAGTGAGC
	Npib-F	TCGGTGCCTCGGTAGTCAGT	803	[17, 18]
	Npib-R	GGGAAGCGGATCCTAGGTCTS
Pi1	RM144-F	TGCCCTGGCGCAAATTTGATCC	234	[19]
Pi1	RM144-R	GCTAGAGGAGATCAGATGGTAGTGCATG
Pikm	Dkm1-F	CTGGAGAGCCTTCCGTGTCGAC	223	[15]
	Dkm1-R	TCTTCACGACCTCAATCCTCCC
	Dkm2-F	GTTGTTCACTCCGTATCTACTACGTC	291	[15]
	Dkm2-R	TTCCTCCGTGATCTCACCAACG
Pikh	Pikh-F	CAATCTCCAAAGTTTTCAGG	216/359	[20]
Pikh	Pikh-R	GCTTCAATCACTGCTAGACC	216/359	[20]

育种单位 Breeding unit	项目 Item	Pi9	Pi40	Pigm	Piz-t	Pita	Pib	Pi1	Pikm	Pikh	总数Total
辽宁省水稻研究所	数目Number	0	1	4	17	18	26	38	35	50	65
LNRRI	比率Ratio/%	0.00	1.54	6.15	26.15	27.69	40.00	58.46	53.85	76.92
盘锦地区及盐碱地研	数目Number	0	0	6	15	14	17	22	29	41	46
究所 PY	比率Ratio/%	0.00	0.00	13.04	32.61	30.43	36.96	47.83	63.04	89.13
沈阳农业大学 SN	数目Number	0	0	2	8	3	4	28	18	33	45
	比率Ratio/%	0.00	0.00	4.44	17.78	6.67	8.89	62.22	40.00	73.33
丹东地区 Dandong	数目Number	0	0	2	19	7	16	12	19	30	30
	比率Ratio/%	0.00	0.00	6.67	63.33	23.33	53.33	40.00	63.33	100.00
抚顺地区 Fushun	数目Number	0	0	2	9	2	1	7	12	12	15
	比率Ratio/%	0.00	0.00	13.33	60.00	13.33	6.67	46.67	80.00	80.00
东亚种业 Dongya	数目Number	0	0	1	5	6	5	10	4	12	13
	比率Ratio/%	0.00	0.00	7.69	38.46	46.15	38.46	76.92	30.77	92.31
营口地区 Yingkou	数目Number	0	0	1	3	1	4	5	6	11	12
	比率Ratio/%	0.00	0.00	8.33	25.00	8.33	33.33	41.67	50.00	91.67
铁岭地区 Tieling	数目Number	0	0	0	5	2	3	7	6	7	10
	比率Ratio/%	0.00	0.00	0.00	50.00	20.00	30.00	70.00	60.00	70.00
庄河地区 Zhuanghe	数目Number	0	0	1	4	0	0	5	2	6	6
	比率Ratio/%	0.00	0.00	16.67	66.67	0.00	0.00	83.33	33.33	100.00
开原地区 Kaiyuan	数目Number	0	0	0	2	2	2	1	1	4	4
	比率Ratio/%	0.00	0.00	0.00	50.00	50.00	50.00	25.00	25.00	100.00
辽宁其他地区 Others	数目Number	0	0	5	11	3	7	10	7	13	14
	比率Ratio/%	0.00	0.00	35.71	78.57	21.43	50.00	71.43	50.00	92.86
总数 Total		0	1	23	97	57	84	144	139	219	260
比率Ratio/%		0.00	0.38	8.85	37.31	21.92	32.31	55.38	53.46	84.23

育种单位 Breeding unit	项目 Item	Pi9	Pi40	Pigm	Piz-t	Pita	Pib	Pi1	Pikm	Pikh	总数Total
辽宁省水稻研究所	数目Number	0	1	4	17	18	26	38	35	50	65
LNRRI	比率Ratio/%	0.00	1.54	6.15	26.15	27.69	40.00	58.46	53.85	76.92
盘锦地区及盐碱地研	数目Number	0	0	6	15	14	17	22	29	41	46
究所 PY	比率Ratio/%	0.00	0.00	13.04	32.61	30.43	36.96	47.83	63.04	89.13
沈阳农业大学 SN	数目Number	0	0	2	8	3	4	28	18	33	45
	比率Ratio/%	0.00	0.00	4.44	17.78	6.67	8.89	62.22	40.00	73.33
丹东地区 Dandong	数目Number	0	0	2	19	7	16	12	19	30	30
	比率Ratio/%	0.00	0.00	6.67	63.33	23.33	53.33	40.00	63.33	100.00
抚顺地区 Fushun	数目Number	0	0	2	9	2	1	7	12	12	15
	比率Ratio/%	0.00	0.00	13.33	60.00	13.33	6.67	46.67	80.00	80.00
东亚种业 Dongya	数目Number	0	0	1	5	6	5	10	4	12	13
	比率Ratio/%	0.00	0.00	7.69	38.46	46.15	38.46	76.92	30.77	92.31
营口地区 Yingkou	数目Number	0	0	1	3	1	4	5	6	11	12
	比率Ratio/%	0.00	0.00	8.33	25.00	8.33	33.33	41.67	50.00	91.67
铁岭地区 Tieling	数目Number	0	0	0	5	2	3	7	6	7	10
	比率Ratio/%	0.00	0.00	0.00	50.00	20.00	30.00	70.00	60.00	70.00
庄河地区 Zhuanghe	数目Number	0	0	1	4	0	0	5	2	6	6
	比率Ratio/%	0.00	0.00	16.67	66.67	0.00	0.00	83.33	33.33	100.00
开原地区 Kaiyuan	数目Number	0	0	0	2	2	2	1	1	4	4
	比率Ratio/%	0.00	0.00	0.00	50.00	50.00	50.00	25.00	25.00	100.00
辽宁其他地区 Others	数目Number	0	0	5	11	3	7	10	7	13	14
	比率Ratio/%	0.00	0.00	35.71	78.57	21.43	50.00	71.43	50.00	92.86
总数 Total		0	1	23	97	57	84	144	139	219	260
比率Ratio/%		0.00	0.38	8.85	37.31	21.92	32.31	55.38	53.46	84.23

所含目标基因数 Number of target genes	品种数量 No. of varieties	比率 Ratio/%	所含目标基因数 Number of target genes	品种数量 No. of varieties	比率 Ratio/%
0	4	1.54	4	46	17.69
1	20	7.69	5	16	6.15
2	72	27.69	6	10	3.85
3	90	34.62	7	2	0.77