湖南水稻主推品种对旱稻孢囊线虫的抗性及评价方法

doi:10.16819/j.1001-7216.2019.8053

摘要/Abstract

摘要：

【目的】旱稻孢囊线虫是一种水稻上重要的病原线虫。筛选抗虫种质资源对抗病品种的选育至关重要。【方法】通过室内二龄幼虫接种和田间自然病圃鉴定,以单株平均孢囊数、相对抗病指数和繁殖系数法评价了湖南省51个主推的水稻品种对旱稻孢囊线虫的抗性。【结果】在供试水稻品种中未发现有免疫和高抗品种。室内接种条件下,在24个中稻品种中,依据单株平均孢囊数评价,仅广两优2010表现为抗;依据相对抗病指数评价,广两优2010、准两优527、广两优1128 3个品种为抗;在27个晚稻品种中,依据单株平均孢囊数评价,盛泰优9712、准两优608、农香优204、岳优9264、岳优3700、中浙优1号、岳优27、湘晚糯1号、贺优50、Y两优9918共10个品种为抗;而依据相对抗病指数评价,湘晚糯1号、贺优50、Y两优9918表现为中感。在田间自然病圃条件下,依据繁殖系数 Pf/Pi评价,筛选出9个晚稻抗性品种,鉴定结果与室内接种相对抗病指数法基本一致。【结论】室内接种相对抗病指数法可以作为评价水稻品种对旱稻孢囊线虫抗性的有效方法。

关键词: 旱稻孢囊线虫, 相对抗病指数, 繁殖系数, 抗性鉴定

Abstract:

【Objective】Heterodera elachista is a new pathogenic nematode of rice. In order to screen the resistant rice cultivars to H. elachista, 【Method】 51 rice varieties from Hunan Province were evaluated by the second-stage juvenile inoculation test in greenhouse and field with average number of cysts per plant, relative resistance index (RRI) and Pf/Pi ratio as indicators. 【Result】No rice cultivar was immune or highly resistant to H. elachista. Among the 24 middle-season rice cultivars, only Guangliangyou 2010 showed resistance to H. elachista based on average number of cysts per plant, and Guangliangyou 2010, Zhunliangyou 527 and Guangliangyou 1128 showed resistance to H. elachista based on RRI evaluation in greenhouse. Among the 27 late rice varieties, Shengtaiyou 9712, Zhunliangyou 608, Nongxiangyou 204, Yueyou 9264, Yueyou 3700, Zhongzheyou 1, Yueyou 27, Xiangwannuo 1, Heyou 50 and Y Liangyou 9918, were resistant to H. elachista based on average number of cyst per plant. However, Xiangwannuo 1, Heyou 50 and Y liangyou 9918 were moderately susceptible based on RRI evaluation. Using Pf/Pi ratio as the evaluation index, nine late resistant varieties screened from greenhouse based on RRI evaluation were basically consistent with field resistance. 【Conclusion】RRI is an efficient index for evaluating the resistance of rice cultivars to rice cyst nematode.

Key words: Heterodera elachista, relative resistance index, Pf/Pi, resistance evaluation

中图分类号:

袁涛, 叶姗, 周建宇, 彭德良, 黄文坤, 丁中. 湖南水稻主推品种对旱稻孢囊线虫的抗性及评价方法[J]. 中国水稻科学, 2019, 33(1): 85-89.

Tao YUAN, Shan YE, Jianyu ZHOU, Deliang PENG, Wenkun HUANG, Zhong DING. Resistance to Heterodera elachista and Evaluation Methods of Major Rice Varieties from Hunan Province, China[J]. Chinese Journal OF Rice Science, 2019, 33(1): 85-89.

图/表 3

参考文献 16

[1]	Ohshima Y. Heterodera elachista n.sp. an upland rice cyst nematode from Japan. Nematol Res, 1974, 4: 51-56.
[2]	Shimizu K.Influence of the upland rice cyst nematode, Heterodera elachista on the yield of the upland-cultured paddy rice. Jpn J Nematol, 1976, 6: 1-6.
[3]	Ding Z, Namphueng J, He X F, Peng D L, Huang W K.First report of the cyst nematode Heterodera elachista on rice in Hunan Province, China. Plant Dis, 2011, 96(1): 151.
[4]	Luca F D, Vovlas N, Lucarelli G, et al.Heterodera elachista the Japanese cyst nematode parasitizing corn in Northern Italy: Integrative diagnosis and bionomics. Eur J Plant Pathol, 2013, 136(4): 857-872.
[5]	王水南, 彭德良, 黄文坤, 丁中. 旱稻孢囊线虫的快速分子检测. 湖南农业大学学报: 自然科学版, 2014, 40(2): 178-182.
	Wang S N, Peng D L, Huang W K, Ding Z.Rapid molecular diagnosis of rice cyst nematode(Heterodera elachista). J Hunan Agric Univ: Nat Sci, 2014, 40(2): 178-182. (in Chinese with English abstract)
[6]	贺沛成, 洪宏, 伍敏敏, 丁中. 旱稻孢囊线虫(Heterodera elachista)孵化特性研究. 植物保护, 2012, 38(1): 101-103.
	Hong P C, Hong H, Wu M M, Ding Z.Study on the hatching characteristics of Heterodera elachista. Plant Prot, 2012, 38(1): 101-103. (in Chinese with English abstract)
[7]	刘炳良, 孙成刚, 王暄, 向桂林, 宋志强, 高菲菲, 李红梅. 小麦品种对禾谷孢囊线虫(Heterodera avenae)江苏沛县群体的抗性鉴定. 麦类作物学报, 2012, 32(3): 563-568.
	Liu B L, Sun C G, Wang X, Xiang G L, Song Z Q, Gao F F, Li H M.Evaluation of the resistance of wheat cultivars to Peixian population of Heterodera avenae from Jiangsu Province, China. J Trit Crops, 2012, 32(3): 563-568. (in Chinese with English abstract)
[8]	Reversat G, Destombes D.Screening for resistance to Heterodera sacchari in the two cultivated rice species, Oryza sativa and O. glaberrima. Fund & Appl Nematol, 1998, 21(4): 307-317.
[9]	郑经武, 程瑚瑞, 方中达. 土壤中线虫孢囊的三种分离方法及综合评价. 植物保护, 1995(1): 50-51.
	Zheng J W, Cheng H R, Fang Z D. Three methods of isolation and comprehensive evaluation of nematode cyst in soil. Plant Prot, 1995(1): 50-51. Chinese with English abstract)
[10]	李永宏, 黄清臻. 新复极差法在生物统计中的应用. 医学动物防制, 2002(5): 270-272.
	Li Y H, Huang Q Z.Application of SSR on the statisties of biology.J Med Pest Control, 2002(5): 270-272. (in Chinese with English abstract)
[11]	Stirling G, Nicol J.Advisory Services for Nematode Pests.Nematologists.org.au, 1999.
[12]	Soriano I R, Schmit V, Brar D S, Prot J C, Reversat G.Resistance to rice root-knot nematode Meloidogyne graminicola identified in Oryza longistaminata and O. glaberrima. Nematology, 1999, 1(4): 395-398.
[13]	丁中, Namphueng J, 何旭峰, 伍敏敏, 洪宏. 旱稻孢囊线虫生活史及侵染特性. 中国水稻科学, 2012, 26(6): 746-750.
	Ding Z, Namphueng J, He X F, Wu M M, Hong H.Life Cycle and infection characteristics of Rice Cyst Nematode, Heterodera elachista Ohshima in rice. Chin J Rice Sci, 2012, 26(6): 746-750. (in Chinese with English abstract)
[14]	苏致衡, 黄文坤, 郑国栋, 张宏嘉, 刘淑艳, 彭德良. 北京地区小麦禾谷孢囊线虫病发生动态调查. 植物保护, 2013, 39(1): 116-120.
	Su Z H, Huang W K, Zheng G D, Zhang H J, Liu S Y, Peng D L.Investigation on the occurrence of Heterodera avenae in Beijing. Plant Prot, 2013, 39(1): 116-120. (in Chinese with English abstract)
[15]	邢小萍, 袁虹霞, 孙君伟, 张洁, 孙炳剑, 李洪连. 河南省小麦主推品种对2种禾谷孢囊线虫的抗性及其评价方法. 作物学报, 2014, 40(5): 805-815.
	Xing X P, Yuan H X, Sun J W, Zhang J, Sun B L, Li H J.Resistance to two species of cereal cyst nematode and evaluation methods in major wheat cultivars from Henan Province, China.Acta Agron Sin, 2014, 40(5): 805-815. (in Chinese with English abstract)
[16]	Seasonal fluctuation and rhizospherical distribution of population of the upland rice cyst nematode,Heterodera elachista.Jpn J Nematol, 1977, 7: 49-57.

品种 Variety	平均单株孢囊数 Mean of cyst per plant	抗性 Resistance	相对抗病指数(RRI) Relative resistance index	抗性 Resistance
广两优2010 Guangliangyou 2010	96.7±11.3	R	0.73	R
准两优527 Zhunliangyou 527	103.9±11.1	MS	0.71	R
广两优1128 Guangliangyou 1128	108.9±7.8	MS	0.70	R
Y两优900 Y liangyou 900	125.9±16.9	MS	0.65	MS
Y两优1998 Y liangyou 1998	141.1±23.9	MS	0.61	MS
Y两优199 Y liangyou 199	149.0±10.9	MS	0.59	MS
隆两优534 Longliangyou 534	151.8±9.8	MS	0.59	MS
和两优1号 Heliangyou 1	155.6±10.5	MS	0.57	MS
C两优386 C liangyou 386	159.0±15.1	MS	0.53	MS
Y两优2号 Y liangyou 2	170.8±8.6	MS	0.52	MS
深两优1号 Shenliangyou 1	204.4±17.4	S	0.43	S
湘两优396 Xiangliangyou 396	211.8±9.1	S	0.41	S
深两优5814 Shenliangyou 5814	215.6±9.9	S	0.40	S
Y两优837 Y liangyou 837	226.1±21.6	S	0.37	S
T优300 T you 300	239.0±20.2	S	0.34	S
深优9595 Shenyou 9595	239.1±18.2	S	0.34	S
奥两优76 Aoliangyou 76	247.4±10.1	S	0.31	S
炳优98 Bingyou 98	249.3±14.1	S	0.31	S
II优416 II you 416	283.3±16.7	S	0.21	HS
荣优390 Rongyou 390	292.6±14.1	S	0.20	HS
C两优4418 C liangyou 4418	308.4±17.4	HS	0.14	HS
新两优6号 Xinliangyou 6	323.3±12.1	HS	0.10	HS
两优3905 Liangyou 3905	337.6±23.7	HS	0.06	HS
隆两优华占 Longliangyouhuazhan	359.9±9.7	HS	0.00	HS

品种 Variety	平均单株孢囊数 Mean of cyst per plant	抗性 Resistance	相对抗病指数(RRI) Relative resistance index	抗性 Resistance
广两优2010 Guangliangyou 2010	96.7±11.3	R	0.73	R
准两优527 Zhunliangyou 527	103.9±11.1	MS	0.71	R
广两优1128 Guangliangyou 1128	108.9±7.8	MS	0.70	R
Y两优900 Y liangyou 900	125.9±16.9	MS	0.65	MS
Y两优1998 Y liangyou 1998	141.1±23.9	MS	0.61	MS
Y两优199 Y liangyou 199	149.0±10.9	MS	0.59	MS
隆两优534 Longliangyou 534	151.8±9.8	MS	0.59	MS
和两优1号 Heliangyou 1	155.6±10.5	MS	0.57	MS
C两优386 C liangyou 386	159.0±15.1	MS	0.53	MS
Y两优2号 Y liangyou 2	170.8±8.6	MS	0.52	MS
深两优1号 Shenliangyou 1	204.4±17.4	S	0.43	S
湘两优396 Xiangliangyou 396	211.8±9.1	S	0.41	S
深两优5814 Shenliangyou 5814	215.6±9.9	S	0.40	S
Y两优837 Y liangyou 837	226.1±21.6	S	0.37	S
T优300 T you 300	239.0±20.2	S	0.34	S
深优9595 Shenyou 9595	239.1±18.2	S	0.34	S
奥两优76 Aoliangyou 76	247.4±10.1	S	0.31	S
炳优98 Bingyou 98	249.3±14.1	S	0.31	S
II优416 II you 416	283.3±16.7	S	0.21	HS
荣优390 Rongyou 390	292.6±14.1	S	0.20	HS
C两优4418 C liangyou 4418	308.4±17.4	HS	0.14	HS
新两优6号 Xinliangyou 6	323.3±12.1	HS	0.10	HS
两优3905 Liangyou 3905	337.6±23.7	HS	0.06	HS
隆两优华占 Longliangyouhuazhan	359.9±9.7	HS	0.00	HS

品种 Variety	平均单株孢囊数 Mean of cyst per plant	抗性 Resistance	相对抗病指数(RRI) Relative resistance index	抗性 Resistance
盛泰优9712 Shengtaiyou 9712	36.3±4.4	R	0.85	R
农香优204 Nongxiangyou 204	36.4±6.9	R	0.85	R
岳优2155 Yueyou 2155	43.6±5.4	R	0.82	R
准两优608 Zhunliangyou 608	47.1±5.4	R	0.81	R
岳优9264 Yueyou 9264	56.1±9.8	R	0.77	R
岳优3700 Yueyou 3700	56.4±10.4	R	0.77	R
中浙优1号 Zhongzheyou 1	58.9±6.3	R	0.76	R
岳优27 Yueyou 27	58.9±9.3	R	0.76	R
H优518 H you 518	71.0±8.7	R	0.71	R
湘晚糯1号 Xiangwannuo 1	88.7±9.3	R	0.63	MS
贺优50 Heyou 50	94.4±17.5	R	0.61	MS
Y两优9918 Y liangyou 9918	99.7±10.5	R	0.59	MS
T优272 T you 272	115.4±14.6	MS	0.52	MS
丰源优299 Fengyuanyou 299	121.6±17.5	MS	0.50	MS
华两优1548 Hualiangyou 1548	123.9±16.6	MS	0.49	S
C两优608 C liangyou 608	124.6±33.6	MS	0.48	S
Y两优19 Y liangyou 19	143.7±19.5	MS	0.41	S
晶两优华占 Jingliangyouhuazhan	145.1±8.8	MS	0.40	S
深优9586 Shenyou 9586	149.0±19.5	MS	0.38	S
T优109 T you 109	153.1±14.5	MS	0.37	S
粤王丝苗Yuewangsimiao	175.9±10.7	MS	0.27	HS
黄华占 Huanghuazhan	178.0±9.4	MS	0.27	HS
丰源优2297 Fengyuanyou 2297	178.0±17.7	MS	0.26	HS
深两优870 Shenliangyou 870	189.6±40.6	MS	0.22	HS
华香优69 Huaxiangyou 69	203.9±27.3	S	0.16	HS
Y两优1号 Y liangyou 1	232.7±33.7	S	0.04	HS
丰源优263 Fengyuanyou 263	241.6±29.9	S	0.00	HS

品种 Variety	平均单株孢囊数 Mean of cyst per plant	抗性 Resistance	相对抗病指数(RRI) Relative resistance index	抗性 Resistance
盛泰优9712 Shengtaiyou 9712	36.3±4.4	R	0.85	R
农香优204 Nongxiangyou 204	36.4±6.9	R	0.85	R
岳优2155 Yueyou 2155	43.6±5.4	R	0.82	R
准两优608 Zhunliangyou 608	47.1±5.4	R	0.81	R
岳优9264 Yueyou 9264	56.1±9.8	R	0.77	R
岳优3700 Yueyou 3700	56.4±10.4	R	0.77	R
中浙优1号 Zhongzheyou 1	58.9±6.3	R	0.76	R
岳优27 Yueyou 27	58.9±9.3	R	0.76	R
H优518 H you 518	71.0±8.7	R	0.71	R
湘晚糯1号 Xiangwannuo 1	88.7±9.3	R	0.63	MS
贺优50 Heyou 50	94.4±17.5	R	0.61	MS
Y两优9918 Y liangyou 9918	99.7±10.5	R	0.59	MS
T优272 T you 272	115.4±14.6	MS	0.52	MS
丰源优299 Fengyuanyou 299	121.6±17.5	MS	0.50	MS
华两优1548 Hualiangyou 1548	123.9±16.6	MS	0.49	S
C两优608 C liangyou 608	124.6±33.6	MS	0.48	S
Y两优19 Y liangyou 19	143.7±19.5	MS	0.41	S
晶两优华占 Jingliangyouhuazhan	145.1±8.8	MS	0.40	S
深优9586 Shenyou 9586	149.0±19.5	MS	0.38	S
T优109 T you 109	153.1±14.5	MS	0.37	S
粤王丝苗Yuewangsimiao	175.9±10.7	MS	0.27	HS
黄华占 Huanghuazhan	178.0±9.4	MS	0.27	HS
丰源优2297 Fengyuanyou 2297	178.0±17.7	MS	0.26	HS
深两优870 Shenliangyou 870	189.6±40.6	MS	0.22	HS
华香优69 Huaxiangyou 69	203.9±27.3	S	0.16	HS
Y两优1号 Y liangyou 1	232.7±33.7	S	0.04	HS
丰源优263 Fengyuanyou 263	241.6±29.9	S	0.00	HS

品种 Cultivar	土中孢囊数 Number of cysts per litre soil			抗性 Resistance
品种 Cultivar	Pi	Pf	Pf/Pi	抗性 Resistance
岳优3700 Yueyou 3700	72.2±6.8	70.2±9.7	0.97	R
岳优2115 Yueyou 2115	65.4±8.9	43.4±11.2	0.67	R
岳优9264 Yueyou 9264	72.0±10.8	66.6±5.6	0.92	R
盛泰优9712 Shengtaiyou 9712	68.2±7.8	43.0±6.9	0.63	R
丰源优2297 Fengyuanyou 2297	67.4±3.6	162.6±4.8	2.43	S
H优518 H you 518	70.6±6.7	58.0±7.3	0.83	R
贺优50 Heyou 50	65.2±9.5	38.0±10.9	0.58	R
中浙优1号 Zhongzheyou 1	68.0±11.9	38.4±8.9	0.57	R
Y两优1号 Y liangyou 1	67.0±3.6	35.0±6.9	0.52	R
准两优608 Zhunliangyou 608	68.2±5.9	18.6±10.6	0.27	R
Y两优9918 Y liangyou 9918	69.4±7.8	38.6±9.6	0.56	R