中国水稻科学 ›› 2019, Vol. 33 ›› Issue (1): 12-19.DOI: 10.16819/j.1001-7216.2019.8054
卓晓轩, 樊琳琳, 安星宇, 郭敬玮, 杨睿, 曾千春, 罗琼*()
收稿日期:
2018-04-28
修回日期:
2018-06-20
出版日期:
2019-01-10
发布日期:
2019-01-10
通讯作者:
罗琼
基金资助:
Xiaoxuan ZHUO, Linlin FAN, Xingyu AN, Jingwei GUO, Rui YANG, Qianchun ZENG, Qiong LUO*()
Received:
2018-04-28
Revised:
2018-06-20
Online:
2019-01-10
Published:
2019-01-10
Contact:
Qiong LUO
摘要:
【目的】子预44是具有广谱持久稻瘟病抗性的云南地方粳稻品种。为了揭示子预44广谱持久抗瘟机制并在抗稻瘟病育种中进行有效利用,【方法】利用江南香糯与子预44杂交构建的遗传群体及分离自云南罗平的稻瘟病菌株LP36进行子预44的抗性遗传分析和抗性基因定位。【结果】子预44对LP36的抗性为单显性基因控制,暂定名为Pi-zy4(t),它位于水稻第4染色体长臂上SSR标记RM5503与RM3276之间约318 kb区间内。【结论】Pi-zy4(t)为新的抗稻瘟病基因。研究结果为子预44的育种利用和Pi-zy4(t)基因的克隆提供了重要信息。
中图分类号:
卓晓轩, 樊琳琳, 安星宇, 郭敬玮, 杨睿, 曾千春, 罗琼. 云南地方品种子预44中一个新的抗稻瘟病基因的定位[J]. 中国水稻科学, 2019, 33(1): 12-19.
Xiaoxuan ZHUO, Linlin FAN, Xingyu AN, Jingwei GUO, Rui YANG, Qianchun ZENG, Qiong LUO. Mapping of a New Rice Blast Resistance Gene in Ziyu 44, a Rice Landrace from Yunnan Province, China[J]. Chinese Journal OF Rice Science, 2019, 33(1): 12-19.
图1 子预44、江南香糯和日本晴接种稻瘟病菌株LP36的表型 Z-子预44;J-江南香糯;N-日本晴。
Fig. 1. Phenotypes of Ziyu 44, Jiangnanxiangnuo and Nipponbare inoculated with M. oryzae isolate LP36. Z, Ziyu 44; J, Jiangnanxiangnuo; N, Nipponbare.
图2 亲本及F2群体单株接种稻瘟病菌株LP36的表型 Z-子预44;J-江南香糯;R-F2群体抗病单株;S-F2群体感病单株。
Fig. 2. Phenotypes of parents and F2 plants inoculated with M. oryzae isolate LP36. Z, Ziyu 44; J, Jiangnanxiangnuo; R, The resistant plants of F2 population; S, The susceptible plants of F2 population.
亲本或世代 Parent or progeny | 总株数 Number of total plants | 抗病单株 Resistant plants | 感病单株 Susceptible plants | 期望比 Expectation ratio | χc2 |
---|---|---|---|---|---|
子预44 Ziyu 44 | 20 | 20 | 0 | ||
江南香糯 Jiangnanxiangnuo | 20 | 0 | 20 | ||
F2 | 149 | 116 | 33 | 3:1 | 0.55 |
表1 江南香糯×子预44的F2群体对LP36的抗性分离情况
Table 1 Resistance segregation to LP36 in F2 population from Ziyu 44/Jiangnanxiangnuo.
亲本或世代 Parent or progeny | 总株数 Number of total plants | 抗病单株 Resistant plants | 感病单株 Susceptible plants | 期望比 Expectation ratio | χc2 |
---|---|---|---|---|---|
子预44 Ziyu 44 | 20 | 20 | 0 | ||
江南香糯 Jiangnanxiangnuo | 20 | 0 | 20 | ||
F2 | 149 | 116 | 33 | 3:1 | 0.55 |
图4 SSR标记RM5503, RM17450和RM3276在F2群体中的分离 Z-子预44;J-江南香糯;1~22-F2感病单株。
Fig. 4. Segregation of RM5503, RM17450 and RM3276 in F2 population. Z, Ziyu 44;J, Jiangnanxiangnuo;1-22, Susceptible plants of F2 population.
登录号 | 位置 | 蛋白 |
---|---|---|
Accession | Location/bp | Protein |
LOC_Os04g51880 | 30 770 723-30 764 486 | GHMP激酶ATP结合蛋白GHMP kinases ATP-binding protein |
LOC_Os04g51890 | 30 783 636-30 784 530 | OsSAUR20-生长素应答SAUR基因家族成员OsSAUR20-Auxin-responsive SAUR gene family member |
LOC_Os04g51900 | 30 791 816-30 797 525 | 表达蛋白Expressed protein |
LOC_Os04g51910 | 30 803 061-30 798 724 | 蛋白酶体/环状体重复包含蛋白Proteasome/cyclosome repeat containing protein |
LOC_Os04g51920 | 30 808 467-30 805 662 | 蛋白质二硫键异构酶Protein disulfide isomerase |
LOC_Os04g51930 | 30 811 261-30 816 943 | 反转录转座子蛋白,LINE亚类Retrotransposon protein, putative, LINE subclass |
LOC_Os04g51940 | 30 821 130-30 825 520 | RNA结合蛋白YT521-B RNA binding protein YT521-B |
LOC_Os04g51950 | 30 829 581-30 825 556 | 丝氨酸/苏氨酸蛋白激酶HT1 Serine/threonine-protein kinase HT1 |
LOC_Os04g51960 | 30 843 035-30 845 776 | 表达蛋白Expressed protein |
LOC_Os04g51970 | 30 856 049-30 852 287 | 突触小泡2相关蛋白Synaptic vesicle 2-related protein |
LOC_Os04g51980 | 30 860 607-30 863 364 | 转移酶家族结构域包含蛋白Transferase family domain containing protein |
LOC_Os04g51990 | 30 869 666-30 871 743 | 转移酶家族结构域包含蛋白Transferase family domain containing protein |
LOC_Os04g52000 | 30 879 563-30 875 218 | 蛋白磷酸酶2C Protein phosphatase 2C |
LOC_Os04g52020 | 30 903 920-30 888 156 | PHD-指结构域包含蛋白PHD-finger domain containing protein |
LOC_Os04g52030 | 30 907 663-30 904 539 | 锌指结构域,LSD1亚类家族蛋白Zinc finger domain, LSD1 subclass family protein |
LOC_Os04g52040 | 30 913 702-30 908 422 | 转换蛋白1 Transition protein 1(TNP1) |
LOC_Os04g52050 | 30 9193 18-30 914 219 | 转换蛋白1 Transition protein 1(TNP1) |
LOC_Os04g52060 | 30 925 574-30 920 554 | 转座因子蛋白Transposable element protein, putative, containing Pfam profile: PF03004, Transposase_24 |
LOC_Os04g52074 | 30 927 631-30 934 100 | 表达蛋白Expressed protein |
LOC_Os04g52090 | 30 939 177-30 940 275 | AP2结构域包含蛋白AP2 domain containing protein |
LOC_Os04g52100 | 30 948 691-30 945 118 | 肽酶,M24家族蛋白Peptidase, M24 family protein |
LOC_Os04g52110 | 30 950 939-30 952 114 | 晚期胚胎发育丰富的蛋白,第3组Late embryogenesis abundant protein, group 3 |
LOC_Os04g52120 | 30 957 619-30 952 498 | 钾转运蛋白Potassium transporter |
LOC_Os04g52130 | 30 961 604-30 965 503 | 粪卟啉原Ⅲ氧化酶,叶绿体前体Coproporphyrinogen Ⅲ oxidase, chloroplast precursor |
LOC_Os04g52140 | 30 974 112-30 979 849 | 蛋白激酶结构域包含蛋白Protein kinase domain containing protein |
LOC_Os04g52164 | 30 995 338-30 999 600 | 转移酶家族蛋白Transferase family protein |
LOC_Os04g52180 | 31 000 118-31 001 986 | 表达蛋白Expressed protein |
LOC_Os04g52190 | 31 003 843-31 008 092 | 液泡分选受体前体Vacuolar-sorting receptor precursor |
LOC_Os04g52200 | 31 013 915-31 007 297 | RNA识别基序包含蛋白RNA recognition motif containing protein |
LOC_Os04g52210 | 31 019 367-31 024 260 | 萜烯合成酶Terpene synthase |
LOC_Os04g52220 | 31 024 637-31 025 064 | 表达蛋白Expressed protein |
LOC_Os04g52230 | 31 026 617-31 031 248 | ent-贝壳杉烯合酶,叶绿体前体ent-kaurene synthase, chloroplast precursor |
LOC_Os04g52240 | 31 035 046-31 043 425 | 萜烯合成酶Terpene synthase |
LOC_Os04g52250 | 31 051 171-31 052 039 | LTPL123-蛋白酶抑制子/种子储存/ LTP家族蛋白前体 LTPL123-protease inhibitor/seed storage/LTP family protein precursor |
LOC_Os04g52260 | 31 061 682-31 062 703 | LTPL124-蛋白酶抑制剂/种子储存/ LTP家族蛋白前体 LTPL124-protease inhibitor/seed storage/LTP family protein precursor |
LOC_Os04g52270 | 31 068 874-31 063 845 | 衣被蛋白ε亚基 ε coatomer subunit epsilon |
LOC_Os04g52280 | 31 069 545-31 075 601 | 脱氢酶Dehydrogenase |
LOC_Os04g52290 | 31 076 344-31 073 380 | PPR重复结构域包含蛋白PPR repeat domain containing protein |
LOC_Os04g52300 | 31 076 461-31 077 928 | 表达蛋白Expressed protein |
LOC_Os04g52310 | 31 080 558-31 078200 | 金属阳离子转运蛋白Metal cation transporter |
表2 第4染色体长臂上候选区间内注释的40个基因
Table 2 The 40 genes in the candidate interval on the long arm of chromosome 4.
登录号 | 位置 | 蛋白 |
---|---|---|
Accession | Location/bp | Protein |
LOC_Os04g51880 | 30 770 723-30 764 486 | GHMP激酶ATP结合蛋白GHMP kinases ATP-binding protein |
LOC_Os04g51890 | 30 783 636-30 784 530 | OsSAUR20-生长素应答SAUR基因家族成员OsSAUR20-Auxin-responsive SAUR gene family member |
LOC_Os04g51900 | 30 791 816-30 797 525 | 表达蛋白Expressed protein |
LOC_Os04g51910 | 30 803 061-30 798 724 | 蛋白酶体/环状体重复包含蛋白Proteasome/cyclosome repeat containing protein |
LOC_Os04g51920 | 30 808 467-30 805 662 | 蛋白质二硫键异构酶Protein disulfide isomerase |
LOC_Os04g51930 | 30 811 261-30 816 943 | 反转录转座子蛋白,LINE亚类Retrotransposon protein, putative, LINE subclass |
LOC_Os04g51940 | 30 821 130-30 825 520 | RNA结合蛋白YT521-B RNA binding protein YT521-B |
LOC_Os04g51950 | 30 829 581-30 825 556 | 丝氨酸/苏氨酸蛋白激酶HT1 Serine/threonine-protein kinase HT1 |
LOC_Os04g51960 | 30 843 035-30 845 776 | 表达蛋白Expressed protein |
LOC_Os04g51970 | 30 856 049-30 852 287 | 突触小泡2相关蛋白Synaptic vesicle 2-related protein |
LOC_Os04g51980 | 30 860 607-30 863 364 | 转移酶家族结构域包含蛋白Transferase family domain containing protein |
LOC_Os04g51990 | 30 869 666-30 871 743 | 转移酶家族结构域包含蛋白Transferase family domain containing protein |
LOC_Os04g52000 | 30 879 563-30 875 218 | 蛋白磷酸酶2C Protein phosphatase 2C |
LOC_Os04g52020 | 30 903 920-30 888 156 | PHD-指结构域包含蛋白PHD-finger domain containing protein |
LOC_Os04g52030 | 30 907 663-30 904 539 | 锌指结构域,LSD1亚类家族蛋白Zinc finger domain, LSD1 subclass family protein |
LOC_Os04g52040 | 30 913 702-30 908 422 | 转换蛋白1 Transition protein 1(TNP1) |
LOC_Os04g52050 | 30 9193 18-30 914 219 | 转换蛋白1 Transition protein 1(TNP1) |
LOC_Os04g52060 | 30 925 574-30 920 554 | 转座因子蛋白Transposable element protein, putative, containing Pfam profile: PF03004, Transposase_24 |
LOC_Os04g52074 | 30 927 631-30 934 100 | 表达蛋白Expressed protein |
LOC_Os04g52090 | 30 939 177-30 940 275 | AP2结构域包含蛋白AP2 domain containing protein |
LOC_Os04g52100 | 30 948 691-30 945 118 | 肽酶,M24家族蛋白Peptidase, M24 family protein |
LOC_Os04g52110 | 30 950 939-30 952 114 | 晚期胚胎发育丰富的蛋白,第3组Late embryogenesis abundant protein, group 3 |
LOC_Os04g52120 | 30 957 619-30 952 498 | 钾转运蛋白Potassium transporter |
LOC_Os04g52130 | 30 961 604-30 965 503 | 粪卟啉原Ⅲ氧化酶,叶绿体前体Coproporphyrinogen Ⅲ oxidase, chloroplast precursor |
LOC_Os04g52140 | 30 974 112-30 979 849 | 蛋白激酶结构域包含蛋白Protein kinase domain containing protein |
LOC_Os04g52164 | 30 995 338-30 999 600 | 转移酶家族蛋白Transferase family protein |
LOC_Os04g52180 | 31 000 118-31 001 986 | 表达蛋白Expressed protein |
LOC_Os04g52190 | 31 003 843-31 008 092 | 液泡分选受体前体Vacuolar-sorting receptor precursor |
LOC_Os04g52200 | 31 013 915-31 007 297 | RNA识别基序包含蛋白RNA recognition motif containing protein |
LOC_Os04g52210 | 31 019 367-31 024 260 | 萜烯合成酶Terpene synthase |
LOC_Os04g52220 | 31 024 637-31 025 064 | 表达蛋白Expressed protein |
LOC_Os04g52230 | 31 026 617-31 031 248 | ent-贝壳杉烯合酶,叶绿体前体ent-kaurene synthase, chloroplast precursor |
LOC_Os04g52240 | 31 035 046-31 043 425 | 萜烯合成酶Terpene synthase |
LOC_Os04g52250 | 31 051 171-31 052 039 | LTPL123-蛋白酶抑制子/种子储存/ LTP家族蛋白前体 LTPL123-protease inhibitor/seed storage/LTP family protein precursor |
LOC_Os04g52260 | 31 061 682-31 062 703 | LTPL124-蛋白酶抑制剂/种子储存/ LTP家族蛋白前体 LTPL124-protease inhibitor/seed storage/LTP family protein precursor |
LOC_Os04g52270 | 31 068 874-31 063 845 | 衣被蛋白ε亚基 ε coatomer subunit epsilon |
LOC_Os04g52280 | 31 069 545-31 075 601 | 脱氢酶Dehydrogenase |
LOC_Os04g52290 | 31 076 344-31 073 380 | PPR重复结构域包含蛋白PPR repeat domain containing protein |
LOC_Os04g52300 | 31 076 461-31 077 928 | 表达蛋白Expressed protein |
LOC_Os04g52310 | 31 080 558-31 078200 | 金属阳离子转运蛋白Metal cation transporter |
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