超表达蔗糖转运蛋白基因OsSUT1对水稻形态和生理的影响

doi:10.16819/j.1001-7216.2018.8015

摘要/Abstract

摘要：

目的磷对植物细胞内蔗糖和淀粉合成有重要影响,它们之间的关系还有许多方面有待阐明。方法本研究利用转基因技术获得OsSUT1超表达材料,通过水培和盆栽实验研究了超表达该基因对蔗糖、磷含量以及植株形态和生理性状的影响。结果转录水平上的表达分析显示,缺磷诱导OsSUT1在水稻根系中表达。水培实验显示,与野生型相比,正常供磷条件下OsSUT1超表达导致水稻植株地上部蔗糖含量下降,同时水稻植株内的磷含量升高;而在缺磷条件下植株体内蔗糖及磷含量均无显著变化。盆栽实验显示,超表达OsSUT1提高了水稻的分蘖数、有效分蘖数、磷含量、粒长和粒宽。结论这些结果说明OsSUT1对水稻的蔗糖和磷含量以及种子的生长发育有重要作用。

关键词: 水稻, 生长发育, OsSUT1, 蔗糖, 磷

Abstract:

【Objective】Phosphorus exerts an important influence on synthesis of sucrose and starch in plant cell. There are still many aspects need to be clarified about their relationship.【Method】In this study, OsSUT1-overexpressed material was obtained by using transgenic technology. Effects of overexpression of this gene on sucrose and phosphorus contents, as well as plant morphology and physiology were investigated under hydroponics and pot experiment conditions. 【Result】Expression analysis at the transcription level indicated that OsSUT1 was induced by phosphorus deficiency in rice roots. Hydroponics experiment showed that compared with the wild-type plant, over-expression of OsSUT1 in the transgenic plant under normal phosphorus supply resulted in a decrease in sucrose content in shoot and an increase in phosphorus content in both root and shoot. However, there was no significant difference in sucrose and phosphorus contents between the OsSUT1-overexpressed and control plants under phosphorus deficiency conditions. Pot experiment showed that overexpression of OsSUT1 increased the number of tillers, effective tillers, and phosphorus content in rice, and the seed size was also larger than that of the wild type. 【Conclusion】These results indicate that OsSUT1 influences sucrose and phosphorus contents and growth and development of the transgenic rice.

Key words: rice, growth and development, OsSUT1, sugar, phosphorus

中图分类号:

Q786
S511.01

冯冰, 孙雅菲, 艾昊, 刘秀丽, 杨晶, 刘璐, 高飞燕, 徐国华, 孙淑斌. 超表达蔗糖转运蛋白基因OsSUT1对水稻形态和生理的影响[J]. 中国水稻科学, 2018, 32(6): 549-556.

Bing FENG, Yafei SUN, Hao AI, Xiuli LIU, Jing YANG, Lu LIU, Feiyan GAO, Guohua XU, Shubin SUN. Overexpression of Sucrose Transporter OsSUT1 Affects Rice Morphology and Physiology[J]. Chinese Journal OF Rice Science, 2018, 32(6): 549-556.

图/表 8

图1 OsSUT1基因在正常供磷和缺磷条件下的相对表达量^图中所显示数据为平均值±标准差(n=3)。柱上标不同字母表示差异达0.05显著水平。

Fig. 1. Relative expression level of OsSUT1 under Pi-sufficient and Pi-deficient conditions.^ Values are mean±SD(n=3) and different letters indicate that the values differ significantly (P < 0.05).

图2 qRT-PCR鉴定OsSUT1-Ox植株超表达效果^图中所显示数据为平均值±标准差(n=3)。柱上标不同字母表示差异未达0.05显著水平。WT–野生型。

Fig. 2. qRT-PCR analysis of the expression levels of OsSUT1 in OsSUT1-Ox plants.^ Values are means±SD(n=3) and different letters indicate that the values differ significantly (P < 0.05). WT, Wild type.

图3 不同供磷条件下野生型和超表达材料蔗糖含量^图中数据为平均值±标准差(n=5)柱上不同小写字母表示差异达0.05显著水平。

Fig. 3. Sucrose concentration of WT and over-expressed plants under Pi-sufficient and Pi-deficient conditions.^ Values are mean±SD (n = 5) and different lowercase letters indicate that the values differ significantly (P < 0.05).

图4 不同供磷条件下野生型与超表达材料表型分析^ A和D,正常供磷(A)和缺磷(D)条件下野生型和OsSUT1超表达材料的苗期表型,标尺为5 cm;图中数据为平均值±标准差,n = 5。柱上标相同小写字母者表示差异未达0.05显著水平。

Fig. 4. Phenotype analysis of wild type(WT) and over-expressed plants under Pi-sufficient and Pi-deficient conditions.^ A and D, Phenotype under Pi-sufficient (A) and Pi-deficient (D) conditions, Bar = 5 cm; Values are mean±SD (n = 5) and different letters indicate that the values differ significantly (P < 0.05).

图5 不同供磷条件下野生型和超表达材料有效磷含量^ A,正常供磷条件下野生型和OsSUT1超表达材料有效磷含量;B,缺磷条件下野生型和OsSUT1超表达材料有效磷含量;图中显示数据为平均值±标准差,n = 5。柱上标相同小写字母者表示差异未达0.05显著水平。

Fig. 5. Pi concentration of wild type(WT) and over-expressed plants under Pi-sufficient and Pi-deficient conditions.^ A, Pi concentration of WT and OsSUT1-Ox materials under Pi-sufficient conditions; B, Pi concentration of WT and OsSUT1-Ox materials under Pi-deficient conditions. Values are mean±SD (n = 5) and different letters indicate that the values differ significantly (P < 0.05).

图6 正常供磷条件下盆栽实验中野生型和超表达材料各部位总磷含量^图中显示数据为平均值±标准差,n = 5。柱上标相同小写字母者表示差异未达0.05显著水平。

Fig. 6. Total P concentration in different tissues of WT and over-expressed plants in Pi-sufficient pot experiments.^ Values are mean±SD (n = 5) and different letters on the histograms indicate that the values differ significantly (P< 0.05).

图7 正常供磷条件下盆栽实验中野生型和超表达材料表型^ A–野生型和OsSUT1突变体材料成熟期盆栽表型,标尺为10 cm;B–成熟期野生型和OsSUT1突变体材料的植株高度;C–成熟期野生型和OsSUT1突变体材料的分蘖数;D,成熟期野生型和OsSUT1突变体材料的有效分蘖数。平均值±标准差,n=10, 柱上标相同字母者表示差异未达0.05显著水平。

Fig. 7. Phenotype of wild type(WT) and over-expressed plants in Pi-sufficient pot experiments.^ A, WT and OsSUT1 mutant in mature stage, Bar = 10cm; B, Plant height of WT and OsSUT1 mutant; C, Tiller number of WT and OsSUT1 mutant; D, Effective tiller number of WT and OsSUT1 mutant. Values are mean±SD (n = 10) and different letters indicate that the values differ significantly (P < 0.05).

图8 野生型与OsSUT1超表达材料种子形态和统计数据^ A和B–野生型和OsSUT1超表达材料籽粒表型;标尺为1 cm。 C–粒长;D–粒宽。生物学重复为20个;图中显示数据为平均值±标准差,n = 20。柱上不同小写字母者表示差异未达0.05显著水平。。

Fig. 8. Seed morphology and statistics of WT and OsSUT1-Ox materials.^ A and B, Grain phenotype of WT and OsSUT1-Ox materials; Bar = 1 cm. C, Grain length; D, Grain width. Values are mean±SD (n=20) and different letters indicate that the values differ significantly (P < 0.05).

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