控释掺混尿素对稻麦产量及氮素利用率的影响

doi:10.16819/j.1001-7216.2017.6143 288

摘要/Abstract

摘要：

【目的】为了探究控释掺混尿素对稻麦产量及氮素利用率的影响,并分析不同氮肥处理下稻麦生长差异及原因,【方法】采用稻麦轮作两季作物大田试验,共设7个处理,包括不施氮CK处理、常规施肥以及掺混控释氮肥10%、20%、40%、80%、100%（依次记为CK,T₁、T₂、T₃、T₄、T₅和T₆处理）,探讨了控释掺混尿素对稻麦干物质积累与分配、干物质增长速率、氮素积累与分配、产量与产量构成因子以及氮素利用率的影响。【结果】掺混控释氮肥20%以上对稻麦生育中后期干物质与氮积累有明显促进作用。但稻麦成熟期干物质与氮分配均呈现出掺混80%以上控释氮肥处理穗部分配明显较少,而茎、叶分配相对较多的特征,表现出一定程度的茎叶徒长,贪青晚熟趋势。添加20%以上比例控释氮肥均具有显著增产的效果,掺混40%控释氮肥处理分别比常规施肥处理增产14.23%(小麦)和10.93%(水稻),分别比单施控释氮肥处理增产7.37%和3.83%。稻麦季随掺混比例增加,氮肥偏生产力、氮肥农学利用率、氮素吸收效率、氮肥表观利用率均呈先增加后减少趋势,以掺混40%控释氮肥处理最优。各施氮处理均以单施控释氮肥处理的氮收获指数最低,小麦与水稻季分别较常规施肥处理下降了3.99%和8.51%。稻麦季土壤氮依存率随掺混比增加呈先下降后回升趋势,且均以掺混40%控释氮肥最低,分别比常规施肥处理下降了14.38%(小麦)和15.84%(水稻)。【结论】控释掺混氮肥处理可协调稻麦氮素吸收与分配,有利于作物生长发育,达到增产和提高氮素利用效率的作用,综合各因素以20%~40%掺混比例为佳。

关键词: 稻麦轮作, 控释掺混尿素, 产量, 氮素利用率

Abstract:

【Objective】A field experiment was conducted to investigate the effect of controlled-release fertilizer(CRF) combined with urea(UR) on the yield, nitrogen use efficiency and growth in wheat-rice rotation system. 【Method】Seven fertilization patterns including non- N fertilization(CK), farmers’ practice(T₁), 10% CRF+90% UR(T2), 20% CRF+80% UR(T₃), 40% CRF+60% UR(T4), 80% CRF+20% UR(T₅), 100% CRF(T₆) were applied to test the effect of different nitrogen treatments on dry matter accumulation and distribution, the growth rate of dry matter, nitrogen accumulation and distribution, yield and yield components, nitrogen use efficiency.【Result】 The dry matter and nitrogen accumulation in the mid-late stage of wheat and rice increased under the treatment with over 20% of controlled release fertilizer. Over 80% CRF addition decreased the dry matter and nitrogen accumulation in rice panicles, but increased accumulation in the stem and leaf, which resulted in late-maturing. Compared with T₁, the yield was increased significantly with over 20% CRF addition, especially in the T₄ treatment. The yield of wheat was increased by 14.23% and for rice, by 10.93%. Compared with T₆, the yield of wheat increased by 7.37% and for rice, by 3.83% respectively. With the increasing CRF addition ratio, the nitrogen partial productivity(NPP), nitrogen agronomy efficiency(NAE), nitrogen uptake efficiency(NUPE), nitrogen apparent utilization efficiency(NAUE) increased both in wheat and rice at beginning and decreased afterwards, with the peak in T₄. In all the nitrogen treatments, the lowest nitrogen harvest index(NHI) was abserved in T₆, the NHI of wheat and rice decreased by 3.99% and 8.51%, respectively. With the increasing CRF ratio, the contribution rate of soil nitrogen(CRSN) in both wheat and rice decreased at beginning and then increased, with the valley in T₄. Compared with T₁, the CRSN of wheat decreased by 14.38% and for rice, by 15.84% under T₄ treatment. 【Conclusion】These results indicated that controlled-release fertilizer enhanced nitrogen absorption and distribution, promoted crop growth and development, as well as crops yield and nitrogen use efficiency. The rational addition ratio of CRF for wheat and rice production ranged frow 20% to 40%.

Key words: rice-wheat rotation, controlled release blend bulk urea, yield, nitrogen use efficiency

中图分类号:

张敬昇, 李冰, 王昌全, 罗晶, 古珺, 龙思帆, 何杰, 向毫, 尹斌. 控释掺混尿素对稻麦产量及氮素利用率的影响[J]. 中国水稻科学, 2017, 31(3): 288-298.

Jingsheng ZHANG, Bing LI, Changquan WANG, Jing LUO, Jun GU, Sifan LONG, Jie HE, Hao XIANG, Bin YIN. Effects of Controlled Release Blend Bulk Urea on the Yield and Nitrogen Use Efficiency of Wheat and Rice[J]. Chinese Journal OF Rice Science, 2017, 31(3): 288-298.

图/表 6

参考文献 35

[1]	张福锁, 王激清, 张卫峰, 崔振岭, 马文奇, 陈新平, 江荣风. 中国主要粮食作物肥料利用率现状与提高途径. 土壤学报, 2008, 45(5): 915-924.
	Zhang F S, Wang J Q, Zhang W F, Cui Z L, Ma W Q, Chen X P, Jiang R F.Nutrient use efficiencies of major cereal crops in China and measures for improvement.Acta Pedol Sin, 2008, 45(5): 915-924. (in Chinese with English abstract)
[2]	朱兆良, 金继运. 保障我国粮食安全的肥料问题. 植物营养与肥料学报, 2013, 19(2): 259-273.
	Zhu Z L, Jin J Y.Fertilizer use and food security in China.Plant Nutr Fert Sci, 2013, 19(2): 259-273. (in Chinese with English abstract)
[3]	Ghaley B B.Uptake and utilization of 5-spilit nitrogen topdressing in animproved and a traditional rice cultivar in the Bhutan Highlands.Exp Agric, 2012, 48: 536-550.
[4]	樊小林, 刘芳, 廖照源, 刘祥洲, 喻建刚. 我国控释肥料研究的现状和展望. 植物营养与肥料学报, 2009, 15(2): 463-473.
	Fan X L, Liu F, Liao Z Y, Liu X Z, Yu J G.The status and outlook for the study of controlled-release fertilizers in China.Plant Nutr Fert Sci, 2009, 15(2): 463-473. (in Chinese with English abstract)
[5]	陈贤友, 吴良欢, 李金先, 应金耀. 新型控释肥对水稻产量与氮肥利用率的影响探讨. 土壤通报, 2010, 41(1): 133-137.
	Chen X Y, Wu L H, Li J X, Ying J Y.Effects of new controlled release fertilizers on rice yield and nitrogen use efficiency.Chin J Soil Sci, 2010, 41(1): 133-137. (in Chinese with English abstract).
[6]	李敏, 郭熙盛, 叶舒娅, 刘枫, 袁嫚嫚, 黄义德. 硫膜和树脂膜控释尿素对水稻产量、光合特性及氮肥利用率的影响. 植物营养与肥料学报, 2013, 19(4): 808-815.
	Li M, Guo X S, Ye S Y, Liu F, Yuan M M, Huang Y D.Effects of sulfur-and polymer-coated controlled release urea on yield,photosynthetic characteristics and nitrogen fertilizer efficiency of rice.Plant Nutr Fert Sci, 2013, 19(4): 808-815. (in Chinese with English abstract)
[7]	古慧娟, 石元亮, 于阁杰, 王晶. 我国缓/控释肥料的应用效应研究进展. 土壤通报, 2011, 42(1): 220-224.
	Gu H J, Shi Y L, Yu G J, Wang J.Research advances on the use efficiency of slow/controlled release fertilizer.Chin J Soil Sci, 2011, 42(1): 220-224. (in Chinese with English abstract)
[8]	辛志远, 王昌全, 申亚珍, 马菲, 周健民, 杜昌文. 水基包衣控释掺混肥料一次性施用对单季稻氮素利用的影响. 农业环境科学学报, 2016, 35(1): 109-114.
	Xin Z Y, Wang C Q, Shen Y Z, Ma F, Zhou J M, Du C W.Effect of single application of water-borne polymer coated controlled-release blend fertilizer on nitrogen utilization in rice.J Agro-Environ Sci, 2016, 35(1): 109-114. (in Chinese with English abstract)
[9]	郭晨, 徐正伟, 李小坤, 任涛, 吴礼树, 邹家龙, 李彬, 鲁剑巍. 不同施氮处理对水稻产量、氮素吸收及利用率的影响. 土壤, 2014, 46(4): 618-622.
	Guo C, Xu Z W, Li X K, Ren T, Wu L S, Zou J L, Li B, Lu J W.Effects of different nitrogen fertilization treatments on yield, nitrogen uptake and use efficiency of rice.Soils, 2014, 46(4): 618-622. (in Chinese with English abstract)
[10]	李伟, 李絮花, 李海燕, 彭强, 刘旭凤, 唐慎欣. 控释尿素与普通尿素混施对夏玉米产量和氮肥效率的影响. 作物学报, 2012, 38(4): 699-706.
	Li W, Li X H, Li H Y, Peng Q, Liu X F, Tang S X.Effects of different mixing ratios of controlled- release urea and common urea on grain yield and nitrogen use efficiency of summer maize.Acta Agron Sin, 2012, 38(4): 699-706. (in Chinese with English abstract)
[11]	张甘霖, 龚子同. 土壤调查实验室分析方法. 北京: 科学出版社, 2012.
	Zhang G L, Gong Z T.Soil survey laboratory methods. Beijing: Science Press, 2012. (in Chinese with English abstract)
[12]	武维华, 张蜀秋, 袁明, 张军, 王学臣. 植物生理学. 北京:科学出版社, 2003.
	Wu W H, Zhang S Q, Yuan M, Zhang J, Wang X C. Plant Physiology.Beijing: Science Press, 2003. (in Chinese with English abstract)
[13]	张福锁,陈新平,陈清. 中国主要农作物施肥指南. 北京: 中国农业大学出版社, 2009.
	Zhang F S, Chen X P, Chen Q.Fertilization guidelines of main crops in China. Beijing: China Agricultural University Press, 2009. (in Chinese with English abstract)
[14]	李云春, 李小坤, 鲁剑巍, 徐维明, 杨运清, 鲁君明. 控释尿素对水稻产量、养分吸收及氮肥利用率的影响. 华中农业大学学报, 2014, 33(3): 46-51.
	Li Y C, Li X K, Lu J W, Xu W M, Yang Y Q, Lu J M.Effects of controlled- release urea on yield, nutrient uptaking and nitrogen use efficiency of rice.J Huazhong Agric Univ, 2014, 33(3): 46-51. (in Chinese with English abstract).
[15]	王维, 蔡一霞, 张建华, 杨建昌, 朱庆森. 适度土壤干旱对贪青小麦茎贮藏碳水化合物向籽粒运转的调节. 作物学报, 2005, 31(3): 289-296.
	Wang W, Cai Y X, Zhang J H, Yang J C, Zhu Q S.Regulation of controlled soil drying on remobilization of stem-stored carbohydrate to grain in wheat grown under unfavorably-delayed senescence.Acta Agron Sin, 2005, 31(3): 289-296. (in Chinese with English abstract)
[16]	陈远学, 陈晓辉, 唐义琴, 张福锁, 陈新平, 张朝春, 刘静, 徐开未. 不同氮用量下小麦/玉米/大豆周年体系的干物质积累和产量变化. 草业学报, 2014, 23(1): 73-83.
	Chen Y X, Chen X H, Tang Y Q, Zhang F S, Chen X P, Zhang C C, Liu J, Xu K W.Effect of nitrogen fertilizer on dry matter accumulation and yield in wheat/ maize/ soybean intercropping systems.Acta Prat Sin, 2014, 23(1): 73-83. (in Chinese with English abstract)
[17]	Abbate P E, Andrade F H, Culot J P.The effects of radiation and nitrogen on number of grains in wheat.J Agric Sci, 1995, 124: 351-360.
[18]	田中伟, 王方瑞, 戴廷波, 蔡剑, 姜东, 曹卫星. 小麦品种改良过程中物质积累转运特性与产量的关系. 中国农业科学, 2012, 45(4): 801-808.
	Tian Z W, Wang F R, Dai T B, Cai J, Jiang D, Cao W X.Characteristics of dry matter accumulation and translocation during the wheat genetic improvement and their relationship to grain yield.Sci Agric Sin, 2012, 45(4): 801-808. (in Chinese with English abstract)
[19]	韦还和, 孟天瑶, 李超, 张洪程, 史天宇, 马荣荣, 王晓燕, 杨筠文, 戴其根, 霍中洋, 许轲, 魏海燕, 郭保卫. 甬优籼粳杂交稻花后干物质积累模型与特征分析. 作物学报, 2016, 42(2): 265-277.
	Wei H H, Meng T Y, Li C, Zhang H C, Shi T Y, Ma R R, Wang X Y, Yang J W, Dai Q G, Huo Z Y, Xu K, Wei H Y, Guo B W.Dynamic model and its characteristics analysis for dry matter production after heading for india/ japonica hybrid rice of Yongyou series.Acta Agron Sin, 2016, 42(2): 265-277. (in Chinese with English abstract)
[20]	汤永禄, 李朝苏, 吴晓丽, 吴春, 杨武云, 黄钢, 马孝玲. 人工合成小麦衍生品种的物质积累、冠层结构及群体光合特性. 中国农业科学, 2014, 47(5): 844-855.
	Tang Y L, Li C S, Wu X L, Wu C, Yang W Y, Huang G, Ma X L.Accumulation of dry matter, canopy structure and photosynthesis of synthetic hexaploid wheat-derived high- yielding varieties grown in Sichuan Basin, China.Sci Agric Sin, 2014, 47(5): 844-855. (in Chinese with English abstract)
[21]	彭玉, 孙永健, 蒋明金, 徐徽, 秦俭, 杨志远, 马均. 不同水分条件下缓/控释氮肥对水稻干无质量和氮素吸收、运转及分配的影响. 作物学报, 2014, 40(5): 859-870.
	Peng Y, Sun Y J, Jiang M J, Xu H, Qin J, Yang Z Y, Ma J.Effects of water management and slow/ controlled release nitrogen fertilizer on biomass and nitrogen accumulation, translocation, and distribution in rice.Acta Agron Sin, 2014, 40(5): 859-870. (in Chinese with English abstract)
[22]	司东霞, 崔振岭, 陈新平, 吕福堂. 不同控释氮肥对夏玉米同化物质积累及氮平衡的影响. 应用生态学报, 2014, 25(6): 1745-1751.
	Si D X, Cui Z L, Chen X P, Lv F T.Effects of controlled release nitrogen fertilizer application on dry matter accumulation and nitrogen balance of summer maize.Chin J Appl Ecol, 2014, 25(6): 1745-1751. (in Chinese with English abstract)
[23]	李录久, 王家嘉, 吴萍萍, 黄厚宽, 蒋荫锡. 秸秆还田下氮肥运筹对白土田水稻产量和氮吸收利用的影响. 植物营养与肥料学报, 2016, 22(1): 254-262.
	Li L J, Wang J J, Wu P P, Huang H K, Jiang Y X.Effect of different nitrogen application on rice yield and N uptake of white soil under wheat straw turnover. Plant turnover.Plant Nutr Fert Sci, 2014, 25(6): 1745-1751. (in Chinese with English abstract)
[24]	孙永健, 孙园园, 刘树金, 杨志远, 程洪彪, 贾现文, 马均. 水分管理和氮肥运筹对水稻养分吸收、转运及分配的影响. 作物学报, 2011, 37(12): 2221-2232.
	Sun Y J, Sun Y Y, Liu S J, Yang Z Y, Cheng H B, Jia X W, Ma J.Effects of management and nitrogen application strategies on nutrient absorption, transfer, and distribution in rice.Acta Agron Sin, 2011, 37(12): 2221-2232. (in Chinese with English abstract)
[25]	郑剑超, 闫曼曼, 张巨松, 高丽丽, 石洪亮, 郑慧, 张玉玲. 遮荫条件下氮肥运筹对棉花生长和氮素积累的影响. 植物营养与肥料学报, 2016, 22(1): 94-103.
	Zheng J C, Yan M M, Zhang J S, Gao L L, Shi H L, Zheng H, Zhang Y L.Effects of nitrogen application on growth and nitrogen accumulation of cotton under shading condition.Plant Nutr Fert Sci, 2016, 22(1): 94-103. (in Chinese with English abstract)
[26]	胡卫丽, 王永华, 李刘霞, 轩红梅, 郭天财. 氮密调控对两种穗型冬小麦品种茎蘖干物质积累与转运的影响. 麦类作物学报, 2014, 34(6): 808-815.
	Hu W L, Wang Y H, Li L X, Xuan H M, Guo T C.Effects of nitrogen and plant density on dry matter accumulation and translocation in main stem and tillers of two spike- types of winter wheat varieties.J Trit Crops, 2014, 34(6): 808-815. (in Chinese with English abstract)
[27]	王寅, 冯国忠, 张天山, 茹铁军, 袁勇, 高强. 控释氮肥与尿素混施对连作春玉米产量、氮素吸收和氮素平衡的影响. 中国农业科学, 2016, 49(3): 518-528.
	Wang Y, Feng G Z, Zhang T S, Ru T J, Yuan Y, Gao Q.Effects of mixed application of controlled- release N fertilizer and common urea on grain yield, N uptake and soil N balance in continuous spring maize production.Sci Agric Sin, 2016, 49(3): 518-528. (in Chinese with English abstract)
[28]	邢晓鸣, 李小春, 丁艳锋, 王绍华, 刘正辉, 唐设, 丁承强, 李刚华, 魏广彬. 缓控释肥组配对机插常规粳稻群体物质生产和产量的影响. 中国农业科学, 2015, 48(24): 4892-4902.
	Xing X M, Li X C, Ding Y F, Wang S H, Liu Z H, Tang S, Ding C Q, Li G H, Wei G B.Effects of types of controlled released nitrogen and fertilization modes on yield and dry mass production.Sci Agric Sin, 2015, 48(24): 4892-4902. (in Chinese with English abstract)
[29]	李国强, 汤亮, 张文宇, 曹卫星, 朱艳. 不同株型小麦干物质积累与分配对氮肥响应的动态分析. 作物学报, 2009, 35(12): 2258-2265.
	Li G Q, Tang L, Zhang W Y, Cao W X, Zhu Y.Dynamic analysis on response of dry matter accumulation and partitioning to nitrogen fertilizer in wheat cultivars with different plant types.Acta Agron Sin, 2009, 35(12): 2258-2265. (in Chinese with English abstract)
[30]	赵朋, 徐凤, 蒋文慧, 齐鹏, 李春莲, 白海波, 吕学莲, 董建力, 王中华. 春小麦旗叶长度、宽度及叶绿素含量QTL分析. 麦类作物学报, 2015, 35(5): 603-608
	Zhao P, Xu F, Jiang W H, Qi P, Li C L, Bai H B, Lv X L, Dong J L, Wang Z H.Quantitative trait loci analysis of flag leaf length, width and chlorophyll content of spring wheat.J Trit Crops, 2015, 35(5): 603-608. (in Chinese with English abstract)
[31]	聂军, 郑圣先, 戴平安, 肖剑, 易国英. 控释氮肥调控水稻光合功能和叶片衰老的生理基础. 中国水稻科学, 2005, 19(3): 255-261.
	Nie J, Zheng S X, Dai P A, Xiao J, Yi G Y.Regulation of senescence and photosynthetic function of rice leaves by controlled release nitrogen fertilizer.Chin J Rice Sci, 2005, 19(3): 255-261. (in Chinese with English abstract).
[32]	Chalk P M, Craswell E T, Polidoro J C, Chen D.Fate and efficiency of 15N labelled slow- and controlled-release fertilizers.Nutr Cyc Agroecosystems, 2015, 102(2): 167-178.
[33]	Yang J C, Zhang J H, Huang Z L, Wang L.Remobilization of carbon reserves is improved by controlled soil-drying during grain filling of wheat.Crop Sci, 2000, 40: 1645-1655.
[34]	伍少福, 韩科峰, 吴良欢, 石其伟, 周永亮, 沈菁, 高兴友, 顾昊男. 控释掺混尿素对水稻产量、品质和氮肥利用率的影响. 浙江农业学报, 2011, 23(5): 983-987.
	Wu S F, Han K F, Wu L H, Shi Q W, Zhou Y L, Shen J, Gao X Y, Gu H N.Effects of application of controlled- releasing bulk blending urea on yield, quality and nitrogen utilization efficiency of rice.Acta Agric Zhejiangensis, 2011, 23(5): 983-987. (in Chinese with English abstract)
[35]	李伟, 李絮花, 董静, 林治安. 冬小麦控释尿素与普通尿素的最佳配比研究. 植物营养与肥料学报, 2014, 20(3): 629-635.
	Li W, Li X H, Dong J, Lin Z A.The appropriate mixing ratio of control- released urea and common urea in winter wheat production.Plant Nutr Fert Sci, 2014, 20(3): 629-635. (in Chinese with English abstract)

作物 Crop	生育期与器官 Stage and organ	处理 Treatment
作物 Crop	生育期与器官 Stage and organ	CK	T₁	T₂	T₃	T₄	T₅	T₆
小麦	分蘖期 Tillering stage
Wheat	地上部 Aboveground	0.50±0.01 e	0.64±0.02 a	0.61±0.03 b	0.58±0.02 c	0.55±0.01 cd	0.54±0.01 de	0.54±0.01 de
	拔节期 Jointing stage
	茎 Culm	1.96±0.03 f	2.22±0.04 d	2.15±0.04 e	2.49±0.03 b	2.57±0.03 a	2.49±0.05 b	2.35±0.05 c
	叶 Leaf	0.38±0.04 d	0.63±0.02 bc	0.61±0.02 bc	0.66±0.02 b	0.71±0.03 a	0.64±0.02 bc	0.58±0.03 c
	地上部 Aboveground	2.33±0.03 f	2.85±0.05 d	2.76±0.05 e	3.14±0.05 b	3.28±0.05 a	3.13±0.03 b	2.93±0.06 c
	抽穗期 Heading stage
	茎 Culm	2.74±0.01 d	3.55±0.01 c	3.45±0.02 c	3.93±0.01 ab	3.96±0.20 a	3.79±0.01 b	3.77±0.03 b
	叶 Leaf	0.39±0.01 d	0.53±0.01 bc	0.50±0.01 c	0.58±0.03 b	0.67±0.04 a	0.58±0.04 b	0.59±0.02 b
	地上部 Aboveground	3.13±0.01 e	4.08±0.02 c	3.95±0.01 d	4.51±0.04 b	4.63±0.16 a	4.37±0.04 b	4.36±0.02 b
	成熟期 Maturity stage
	茎 Culm	3.56±0.01 d	4.35±0.03 c	4.44±0.12 bc	4.72±0.04 a	4.86±0.15 a	4.70±0.02 a	4.54±0.04 b
	叶 Leaf	0.29±0.01 c	0.51±0.02 b	0.51±0.02 b	0.50±0.03 b	0.48±0.02 b	0.47±0.03 b	0.70±0.02 a
	穗Panicle	2.34±0.06 d	4.90±0.09 c	4.68±0.26 c	5.38±0.10 ab	5.60±0.18 a	5.33±0.12 ab	5.21±0.12 b
	地上部 Aboveground	6.19±0.05 d	9.76±0.07 c	9.63±0.26 c	10.59±0.06 b	10.94±0.09 a	10.50±0.11 b	10.45±0.11 b
水稻	分蘖期 Tillering stage
Rice	地上部 Aboveground	0.49±0.01 d	0.57±0.05 bc	0.52±0.01 cd	0.61±0.02 b	0.75±0.03 a	0.72±0.04 a	0.63±0.05 b
	拔节期 Jointing stage
	茎 Culm	2.24±0.02 d	2.74±0.01 bc	2.79±0.01 ab	2.82±0.01 a	2.88±0.02 a	2.83±0.01 a	2.71±0.09 c
	叶 Leaf	1.21±0.01 d	1.63±0.06 c	1.57±0.12 c	1.75±0.01 b	1.81±0.02 ab	1.90±0.03 a	1.82±0.05 ab
	地上部 Aboveground	3.45±0.03 d	4.36±0.07 c	4.36±0.12 c	4.56±0.02 b	4.69±0.02 a	4.73±0.03 a	4.53±0.05 b
	抽穗期 Heading stage
	茎 Culm	5.76±0.08 c	6.56±0.03 b	6.57±0.11 b	6.92±0.05 a	7.03±0.05 a	6.99±0.10 a	7.09±0.06 a
	叶 Leaf	1.43±0.02 d	1.90±0.18 c	2.13±0.11 abc	2.19±0.16 ab	2.07±0.05 abc	2.01±0.02 bc	2.31±0.12 a
	地上部 Aboveground	7.20±0.08 e	8.46±0.19 d	8.70±0.11 c	9.11±0.12 b	9.11±0.01 b	9.01±0.11 b	9.40±0.06 a
	成熟期 Maturity stage
	茎 Culm	3.56±0.03 e	4.44±0.05 c	4.31±0.06 d	4.44±0.03 c	4.58±0.01 b	4.73±0.02 a	4.75±0.03 a
	叶 Leaf	1.14±0.03 d	1.21±0.05 d	1.39±0.06 c	1.53±0.10 b	1.51±0.02 b	1.59±0.02 ab	1.66±0.03 a
	穗Panicle	6.72±0.10 d	7.89±0.05 c	7.78±0.09 c	8.63±0.19 ab	8.75±0.08 a	8.60±0.16 ab	8.43±0.08 b
	地上部 Aboveground	11.41±0.11 c	13.55±0.07 b	13.48±0.15 b	14.61±0.22 a	14.85±0.06 a	14.92±0.17 a	14.85±0.07 a

作物 Crop	生育期与器官 Stage and organ	处理 Treatment
作物 Crop	生育期与器官 Stage and organ	CK	T₁	T₂	T₃	T₄	T₅	T₆
小麦	分蘖期 Tillering stage
Wheat	地上部 Aboveground	0.50±0.01 e	0.64±0.02 a	0.61±0.03 b	0.58±0.02 c	0.55±0.01 cd	0.54±0.01 de	0.54±0.01 de
	拔节期 Jointing stage
	茎 Culm	1.96±0.03 f	2.22±0.04 d	2.15±0.04 e	2.49±0.03 b	2.57±0.03 a	2.49±0.05 b	2.35±0.05 c
	叶 Leaf	0.38±0.04 d	0.63±0.02 bc	0.61±0.02 bc	0.66±0.02 b	0.71±0.03 a	0.64±0.02 bc	0.58±0.03 c
	地上部 Aboveground	2.33±0.03 f	2.85±0.05 d	2.76±0.05 e	3.14±0.05 b	3.28±0.05 a	3.13±0.03 b	2.93±0.06 c
	抽穗期 Heading stage
	茎 Culm	2.74±0.01 d	3.55±0.01 c	3.45±0.02 c	3.93±0.01 ab	3.96±0.20 a	3.79±0.01 b	3.77±0.03 b
	叶 Leaf	0.39±0.01 d	0.53±0.01 bc	0.50±0.01 c	0.58±0.03 b	0.67±0.04 a	0.58±0.04 b	0.59±0.02 b
	地上部 Aboveground	3.13±0.01 e	4.08±0.02 c	3.95±0.01 d	4.51±0.04 b	4.63±0.16 a	4.37±0.04 b	4.36±0.02 b
	成熟期 Maturity stage
	茎 Culm	3.56±0.01 d	4.35±0.03 c	4.44±0.12 bc	4.72±0.04 a	4.86±0.15 a	4.70±0.02 a	4.54±0.04 b
	叶 Leaf	0.29±0.01 c	0.51±0.02 b	0.51±0.02 b	0.50±0.03 b	0.48±0.02 b	0.47±0.03 b	0.70±0.02 a
	穗Panicle	2.34±0.06 d	4.90±0.09 c	4.68±0.26 c	5.38±0.10 ab	5.60±0.18 a	5.33±0.12 ab	5.21±0.12 b
	地上部 Aboveground	6.19±0.05 d	9.76±0.07 c	9.63±0.26 c	10.59±0.06 b	10.94±0.09 a	10.50±0.11 b	10.45±0.11 b
水稻	分蘖期 Tillering stage
Rice	地上部 Aboveground	0.49±0.01 d	0.57±0.05 bc	0.52±0.01 cd	0.61±0.02 b	0.75±0.03 a	0.72±0.04 a	0.63±0.05 b
	拔节期 Jointing stage
	茎 Culm	2.24±0.02 d	2.74±0.01 bc	2.79±0.01 ab	2.82±0.01 a	2.88±0.02 a	2.83±0.01 a	2.71±0.09 c
	叶 Leaf	1.21±0.01 d	1.63±0.06 c	1.57±0.12 c	1.75±0.01 b	1.81±0.02 ab	1.90±0.03 a	1.82±0.05 ab
	地上部 Aboveground	3.45±0.03 d	4.36±0.07 c	4.36±0.12 c	4.56±0.02 b	4.69±0.02 a	4.73±0.03 a	4.53±0.05 b
	抽穗期 Heading stage
	茎 Culm	5.76±0.08 c	6.56±0.03 b	6.57±0.11 b	6.92±0.05 a	7.03±0.05 a	6.99±0.10 a	7.09±0.06 a
	叶 Leaf	1.43±0.02 d	1.90±0.18 c	2.13±0.11 abc	2.19±0.16 ab	2.07±0.05 abc	2.01±0.02 bc	2.31±0.12 a
	地上部 Aboveground	7.20±0.08 e	8.46±0.19 d	8.70±0.11 c	9.11±0.12 b	9.11±0.01 b	9.01±0.11 b	9.40±0.06 a
	成熟期 Maturity stage
	茎 Culm	3.56±0.03 e	4.44±0.05 c	4.31±0.06 d	4.44±0.03 c	4.58±0.01 b	4.73±0.02 a	4.75±0.03 a
	叶 Leaf	1.14±0.03 d	1.21±0.05 d	1.39±0.06 c	1.53±0.10 b	1.51±0.02 b	1.59±0.02 ab	1.66±0.03 a
	穗Panicle	6.72±0.10 d	7.89±0.05 c	7.78±0.09 c	8.63±0.19 ab	8.75±0.08 a	8.60±0.16 ab	8.43±0.08 b
	地上部 Aboveground	11.41±0.11 c	13.55±0.07 b	13.48±0.15 b	14.61±0.22 a	14.85±0.06 a	14.92±0.17 a	14.85±0.07 a

作物 Crop	生育期与器官 Stage and organ	处理 Treatment
作物 Crop	生育期与器官 Stage and organ	CK	T₁	T₂	T₃	T₄	T₅	T₆
小麦	分蘖期 Tillering stage
Wheat	地上部 Aboveground	8.50±2.22 c	15.54±0.81 a	13.00±0.32 b	12.60±0.70 b	12.06±0.47 b	12.00±0.41 b	11.39±0.42 b
	拔节期 Jointing stage
	茎 Culm	6.11±1.13 b	9.97±1.55 a	12.63±1.76 a	12.84±1.30 a	14.35±1.66 a	11.59±2.31 a	10.98±3.13 a
	叶 Leaf	3.59±0.41 c	9.61±1.11 b	10.32±1.81 b	12.33±1.83 a	13.10±0.21 a	9.83±0.92 b	9.23±0.46 b
	地上部 Aboveground	9.70±0.83 c	19.58±2.42 b	22.95±3.06 ab	25.17±2.30 ab	27.45±1.86 a	21.43±2.84 b	20.21±3.27 b
	抽穗期 Maturity stage
	茎 Culm	6.74±1.89 d	12.22±1.67 c	13.02±0.33 c	15.81±1.23 b	19.99±1.00 a	20.10±2.56 a	18.28±1.29 ab
	叶 Leaf	3.15±0.51 c	6.97±0.87 ab	5.73±1.06 b	7.38±0.91 ab	8.42±0.35 a	7.97±0.94 a	7.87±0.82 a
	地上部 Aboveground	9.89±2.38 d	19.19±2.36 c	18.74±1.29 c	23.20±2.13 bc	28.41±1.30 a	28.07±3.46 a	26.15±1.48 ab
	成熟期 Maturity stage
	茎 Culm	6.39±0.72 c	11.98±0.34 b	11.85±0.92 b	14.27±0.61 a	14.43±0.45 a	14.46±1.31 a	14.87±0.63 a
	叶 Leaf	3.15±0.51 b	6.58±0.47 a	6.34±0.15 a	6.66±0.37 a	7.53±0.64 a	6.95±0.74 a	7.57±0.73 a
	穗Panicle	16.63±1.30 f	41.18±0.56 d	39.25±0.23 e	45.92±0.19 b	47.80±0.49 a	45.06±0.90 bc	43.92±0.66 c
	地上部 Aboveground	26.17±1.56 e	59.74±0.21 c	57.43±0.90 d	66.85±0.75 b	69.77±1.35 a	66.47±0.28 b	66.36±0.66 b
水稻	分蘖期 Tillering stage
Rice	地上部 Aboveground	5.73±0.41 d	9.72±0.75 a	7.34±0.18 c	8.16±0.39 bc	9.50±0.55 a	8.96±0.45 ab	7.64±0.50 c
	拔节期 Jointing stage
	茎 Culm	10.45±0.61 f	13.71±0.21 cd	11.79±0.70 e	14.68±0.50 c	19.43±0.45 a	15.83±0.59 b	13.13±0.88 d
	叶 Leaf	14.13±0.76 e	19.11±1.71 d	19.86±2.36 cd	21.96±0.23 bc	26.76±0.30 a	24.32±0.71 b	22.93±0.75 b
	地上部 Aboveground	24.58±1.32 e	32.82±1.89 d	31.65±2.19 d	36.64±0.56 c	46.19±0.67 a	40.14±0.64 b	36.06±0.23 c
	抽穗期 Heading stage
	茎 Culm	19.87±1.10 d	26.27±1.70 c	20.03±3.73 d	28.79±1.77 bc	34.47±1.11 a	32.09±1.29 ab	30.38±0.36 b
	叶 Leaf	13.26±0.95 c	20.88±0.82 b	21.57±1.59 b	25.62±1.53 a	26.77±0.63 a	26.08±0.65 a	28.10±2.09 a
	地上部 Aboveground	33.13±2.05 e	47.15±1.17 c	41.60±4.76 d	54.41±3.26 b	61.24±1.20 a	58.18±1.22 ab	58.48±2.45 ab
	成熟期 Maturity stage
	茎 Culm	5.45±0.25 d	9.74±0.67 c	9.35±0.59 c	10.98±0.21 b	11.48±0.31 ab	11.95±0.42 a	12.28±0.06 a
	叶 Leaf	5.33±0.39 f	9.40±0.50 e	10.52±0.36 d	11.95±0.11 c	12.93±0.36 b	13.81±0.42 a	14.31±0.27 a
	穗Panicle	34.75±1.07 e	51.37±1.20 d	51.81±0.80 d	54.08±1.06 c	59.35±1.15 a	56.53±1.21 b	53.16±0.67 cd
	地上部 Aboveground	45.54±1.71 e	70.51±2.31 d	71.68±0.21 d	77.01±1.02 c	83.77±1.18 a	82.28±1.35 a	79.76±0.94 b

作物 Crop	生育期与器官 Stage and organ	处理 Treatment
作物 Crop	生育期与器官 Stage and organ	CK	T₁	T₂	T₃	T₄	T₅	T₆
小麦	分蘖期 Tillering stage
Wheat	地上部 Aboveground	8.50±2.22 c	15.54±0.81 a	13.00±0.32 b	12.60±0.70 b	12.06±0.47 b	12.00±0.41 b	11.39±0.42 b
	拔节期 Jointing stage
	茎 Culm	6.11±1.13 b	9.97±1.55 a	12.63±1.76 a	12.84±1.30 a	14.35±1.66 a	11.59±2.31 a	10.98±3.13 a
	叶 Leaf	3.59±0.41 c	9.61±1.11 b	10.32±1.81 b	12.33±1.83 a	13.10±0.21 a	9.83±0.92 b	9.23±0.46 b
	地上部 Aboveground	9.70±0.83 c	19.58±2.42 b	22.95±3.06 ab	25.17±2.30 ab	27.45±1.86 a	21.43±2.84 b	20.21±3.27 b
	抽穗期 Maturity stage
	茎 Culm	6.74±1.89 d	12.22±1.67 c	13.02±0.33 c	15.81±1.23 b	19.99±1.00 a	20.10±2.56 a	18.28±1.29 ab
	叶 Leaf	3.15±0.51 c	6.97±0.87 ab	5.73±1.06 b	7.38±0.91 ab	8.42±0.35 a	7.97±0.94 a	7.87±0.82 a
	地上部 Aboveground	9.89±2.38 d	19.19±2.36 c	18.74±1.29 c	23.20±2.13 bc	28.41±1.30 a	28.07±3.46 a	26.15±1.48 ab
	成熟期 Maturity stage
	茎 Culm	6.39±0.72 c	11.98±0.34 b	11.85±0.92 b	14.27±0.61 a	14.43±0.45 a	14.46±1.31 a	14.87±0.63 a
	叶 Leaf	3.15±0.51 b	6.58±0.47 a	6.34±0.15 a	6.66±0.37 a	7.53±0.64 a	6.95±0.74 a	7.57±0.73 a
	穗Panicle	16.63±1.30 f	41.18±0.56 d	39.25±0.23 e	45.92±0.19 b	47.80±0.49 a	45.06±0.90 bc	43.92±0.66 c
	地上部 Aboveground	26.17±1.56 e	59.74±0.21 c	57.43±0.90 d	66.85±0.75 b	69.77±1.35 a	66.47±0.28 b	66.36±0.66 b
水稻	分蘖期 Tillering stage
Rice	地上部 Aboveground	5.73±0.41 d	9.72±0.75 a	7.34±0.18 c	8.16±0.39 bc	9.50±0.55 a	8.96±0.45 ab	7.64±0.50 c
	拔节期 Jointing stage
	茎 Culm	10.45±0.61 f	13.71±0.21 cd	11.79±0.70 e	14.68±0.50 c	19.43±0.45 a	15.83±0.59 b	13.13±0.88 d
	叶 Leaf	14.13±0.76 e	19.11±1.71 d	19.86±2.36 cd	21.96±0.23 bc	26.76±0.30 a	24.32±0.71 b	22.93±0.75 b
	地上部 Aboveground	24.58±1.32 e	32.82±1.89 d	31.65±2.19 d	36.64±0.56 c	46.19±0.67 a	40.14±0.64 b	36.06±0.23 c
	抽穗期 Heading stage
	茎 Culm	19.87±1.10 d	26.27±1.70 c	20.03±3.73 d	28.79±1.77 bc	34.47±1.11 a	32.09±1.29 ab	30.38±0.36 b
	叶 Leaf	13.26±0.95 c	20.88±0.82 b	21.57±1.59 b	25.62±1.53 a	26.77±0.63 a	26.08±0.65 a	28.10±2.09 a
	地上部 Aboveground	33.13±2.05 e	47.15±1.17 c	41.60±4.76 d	54.41±3.26 b	61.24±1.20 a	58.18±1.22 ab	58.48±2.45 ab
	成熟期 Maturity stage
	茎 Culm	5.45±0.25 d	9.74±0.67 c	9.35±0.59 c	10.98±0.21 b	11.48±0.31 ab	11.95±0.42 a	12.28±0.06 a
	叶 Leaf	5.33±0.39 f	9.40±0.50 e	10.52±0.36 d	11.95±0.11 c	12.93±0.36 b	13.81±0.42 a	14.31±0.27 a
	穗Panicle	34.75±1.07 e	51.37±1.20 d	51.81±0.80 d	54.08±1.06 c	59.35±1.15 a	56.53±1.21 b	53.16±0.67 cd
	地上部 Aboveground	45.54±1.71 e	70.51±2.31 d	71.68±0.21 d	77.01±1.02 c	83.77±1.18 a	82.28±1.35 a	79.76±0.94 b

处理 Treatment		穗长 Spike length /cm					旗叶(剑叶)长 FLL /cm		旗叶(剑叶)面积 FLA /cm²
小麦Wheat
CK	2339.33±59.13 d	5.44±0.14 e	11.67±1.16 b	35.82±2.45 a	-	14.97±0.16 c		1.58±0.04 b	19.60±0.71 d
T₁	4898.67±88.31 c	6.67±0.29 d	38.00±2.00 a	40.30±1.33 a	-	15.90±0.42 ab		1.65±0.01 a	21.73±0.53 bc
T₂	4670.80±260.00 c	6.48±0.03 d	38.33±5.69 a	40.20±2.44 a	-	15.68±0.38 b		1.63±0.01 a	21.26±0.60 c
T₃	5375.47±102.88 ab	7.13±0.37 c	37.67±3.51 a	39.82±3.03 a	-	16.43±0.11 a		1.65±0.01 a	22.54±0.18 ab
T₄	5595.73±184.84 a	7.95±0.20 a	34.00±3.00 a	41.07±1.79 a	-	16.46±0.14 a		1.67±0.02 a	22.85±0.02 a
T₅	5325.73±122.38 ab	7.67±0.25 ab	39.00±7.00 a	40.00±2.08 a	-	16.35±0.20 a		1.67±0.01 a	22.71±0.20 ab
T₆	5211.87±121.60 b	7.37±0.23 bc	41.00±8.00 a	39.72±0.60 a	-	16.10±0.07 ab		1.67±0.01 a	22.36±0.25 ab
水稻Rice
CK	6719.92±104.16 d	24.46±1.38 b	168.67±2.31 d	27.17±0.36 c	85.13±0.01 d		33.94±0.65 d	1.39±0.03 b	35.29±0.12 d
T₁	7890.32±50.66 c	30.05±0.82 a	191.00±5.29 bc	27.53±0.12 c	88.71±0.01 c		35.50±0.27 c	1.51±0.02 a	40.29±0.71 c
T₂	7776.66±86.26 c	29.37±1.09 ab	178.33±4.51 cd	27.55±0.24 c	89.49±0.01 bc		35.54±0.44 c	1.50±0.02 a	39.90±0.89 c
T₃	8634.78±187.04 ab	31.66±2.35 a	202.67±11.55 b	29.48±0.56 a	91.41±0.01 ab		35.74±0.30 c	1.53±0.03 a	40.91±0.46 bc
T₄	8752.50±83.34 a	32.74±3.02 a	217.33±7.51 a	29.85±0.44 a	92.32±0.01 a		36.99±0.20 a	1.54±0.02 a	42.81±0.74 a
T₅	8603.71±159.04 ab	32.39±4.02 a	222.67±10.79 a	28.57±0.21 b	91.16±0.01 ab		36.58±0.33 ab	1.55±0.04 a	42.43±0.79 a
T₆	8430.43±80.65 b	27.01±2.01 ab	229.00±6.08 a	27.55±0.52 c	89.43±0.01 bc		35.97±0.38 bc	1.55±0.01 a	41.82±0.50 ab