灌浆期夜间高温胁迫下耐热和热敏感水稻籽粒的比较蛋白质组分析

doi:10.16819/j.1001.7216.2017.6101

摘要/Abstract

摘要：

目的为了阐明水稻灌浆期耐夜间高温的分子机制,提高水稻耐热性,鉴定了水稻近等基因系耐热纯系XN0437T与热敏感纯系XN0437S在灌浆期夜间高温胁迫下的差异表达蛋白质。方法采用桶栽方法培育水稻,于开花期标记同一天扬花的颖花以保障所取颖花样品的生育进程一致;于水稻灌浆初期(花后第8天)进行夜间高温处理。高温处理结束后剪取带标记的颖花提取水稻籽粒总蛋白质,采用8-plex iTRAQ试剂盒进行蛋白质样品的差异显色标记,标记样品采用高效液相系统进行质谱鉴定及定量分析。结果鉴定并定量了3130个蛋白质,耐热与热敏感水稻纯系间存在36个差异表达蛋白质。蛋白质功能注释结果显示,鉴定的36个差异表达蛋白质中仅14个蛋白质(占38.9%)注释了功能,12个蛋白质(占33.3%)为推测性功能注释, 10个(占27.8%)为功能未知的蛋白质。已注释功能的14个蛋白质中,5个蛋白质参与能量代谢,3个蛋白质参与物质转运与代谢,2个蛋白质参与光合作用,3个蛋白质为响应逆境的锌指蛋白质,1个蛋白质为响应逆境的热激蛋白质。结论灌浆期夜间高温影响水稻籽粒细胞内参与能量代谢、物质转运与代谢、光合作用等相关蛋白质的表达模式。水稻籽粒细胞中锌指蛋白质Q67TK9、Q10N88上调表达,锌指蛋白质Q5YLY5下调表达,有利于提高水稻灌浆期对夜间高温的耐热性。

关键词: 水稻, 灌浆期, 夜间高温, 蛋白质组学, iTRAQ技术

Abstract:

【Objective】To understand molecular mechanism of rice tolerance to high night temperature at filling stage,we identify the differentially expressed proteins (DEPs) and screen the proteins involving in regulating the heat-tolerance in rice. 【Method】The heat-tolerant rice line XN0437T and the heat-sensitive rice line XN0437S originated from a near-genetic lines were used as plant materials. Rice was pot-cultivated under traditional management. To ensure that only grain samples with uniform growth were used for proteomes analysis, rice panicle bloomed on the same date were labeled with same markers and spikelets flowering on the same date on the labeled panicle were further labeled. On the 8th day after flowering, rice plants with same label were transferred to chambers for high night temperature treatment.Rice grains with same label were harvested and total proteins of the grain samples were extracted after high temperature treatment. The 8-plex iTRAQ kits combined with the LC-MS/MS technology were used to analyze the DEPs between the heat-tolerant and heat-sensitive lines. 【Result】After protein’s databases searching and differentially expressed proteins analysis, 3130 proteins were finally identified and 36 proteins showed differential expression levels between the heat-tolerant and heat-sensitive line. For the 36 DEPs, 14 proteins (38.9%) had functional annotation, 12 (33.3%) had putative function, and 10 (33.3%) were functional unknown proteins. The 14 function-known proteins were involved in energy metabolism (five proteins), transport and metabolism (three proteins), photosynthesis (two proteins) and defense response (four proteins). 【Conclusion】High night temperature impacts the expressed patterns of the proteins involved in energy metabolism, matter transport and metabolism, photosynthesis and defense response in rice at filling stage. We suggested that up-regulated expression of the zinc finger proteins (Q67TK9 and Q10N88), and down-regulated expression of the zinc finger protein (Q5YLY5) in rice grain could enhance the heat-tolerance in rice.

Key words: rice, filling stage, high night temperature, proteomics, iTRAQ technique

黄小平, 张宏玉, 雷刚, 王志美, 章智, 贺超, 廖江林, 黄英金. 灌浆期夜间高温胁迫下耐热和热敏感水稻籽粒的比较蛋白质组分析[J]. 中国水稻科学, 2017, 31(1): 13-22.

Xiaoping HUANG, Hongyu ZHANG, Gang LEI, Zhimei WANG, Zhi ZHANG, Chao HE, Jianglin LIAO, Yingjin HUANG. Analysis on Comparative Proteomics of Rice Grain Between Heat-tolerant and Heat-sensitive Lines Under High Night Temperature Stress at Filling Stage[J]. Chinese Journal OF Rice Science, 2017, 31(1): 13-22.

图/表 4

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蛋白质ID Protein ID	基因ID Gene ID	HT	HS	HT/HS	蛋白质的功能描述 Functional illustration of protein
能量代谢 Energy metabolism
Q9FU69	LOC_Os01g08090	-1.613	-2.899	1.800	糖基转移酶 Glycosyltransferase
Q7Y179	LOC_Os03g46660	1.794	1.000	1.793	葡聚糖-1,3-β-葡萄糖苷内切酶7 Glucan endo-1,3-beta-glucosidase 7
Q0IPF8	LOC_Os12g10570	-1.495	1.012	-1.513	ATP合成酶亚基β ATP synthase subunit beta
Q2R1N0	LOC_Os11g38650	-1.739	-1.085	-1.605	糖基转移酶 Glycosyltransferase
Q84T00	LOC_Os03g58800	-1.805	1.044	-1.887	ATP酶家族蛋白质 ATPase, AAA family protein
物质转运与代谢 Matter translocation and metabolism
Q8GTK2	LOC_Os07g46350	1.832	1.097	1.669	羧肽酶 Carboxypeptidase
Q7Y007	LOC_Os03g47940	2.076	1.330	1.561	类似GDSL脂肪酶或酰基水解酶家族蛋白质 GDSL-like Lipase/Acylhydrolase family protein
Q10BU2	LOC_Os03g58980	-1.672	1.267	-2.119	类似(种子)萌发蛋白质3-7 Germin-like protein 3-7
光合作用 Photosynthesis
Q2R237	LOC_Os11g37130	3.115	1.199	2.598	叶绿体独立转位酶蛋白质 TATB Sec-independent protein translocase protein TATB, chloroplastic
Q658I1	LOC_Os06g03790	-1.667	1.674	-2.793	核糖体蛋白质L29 Ribosomal protein L29
逆境应答 Stress response
Q67TK9	B1012G11.2	2.852	-1.041	2.967	锌指蛋白质 Zinc knuckle containing protein-like
Q10N88	LOC_Os03g17020	2.341	1.326	1.765	ZIGA2锌指蛋白质 ARFGAP-like zinc finger-containing protein ZIGA2
Q84Q72	LOC_Os03g16030	1.906	2.975	-1.560	18.1 kD热激蛋白质类型Ⅰ 18.1 kD class Ⅰ heat shock protein
Q5YLY5	LOC_Os06g50840	-4.167	-1.560	-2.667	锌指蛋白质 Zinc finger protein-like
推测性功能注释蛋白质 Preliminary annotation of unknown-function protein
Q6ZJ47	LOC_Os08g02550	3.150	-1.522	4.796	可能的COP9复合亚基3 Putative COP9 complex subunit 3, FUS11
Q6ZCC9	LOC_Os08g02030	1.842	-1.764	3.251	可能的AER蛋白质 Putative AER
Q6ZCP8	LOC_Os08g07060	2.046	-1.477	3.020	可能的泛素氧化还原酶亚基1 Putative ubiquinone oxidoreductase subunit 1
Q6I587	LOC_Os05g5081	2.045	1.057	1.934	可能的钙依赖蛋白质激酶 Putative calcium-dependent protein kinase
Q53M16	LOC_Os11g13690	1.109	-1.560	1.730	假定蛋白质 Hypothetical protein
Q9LWS6	LOC_Os06g02470	1.699	-1.003	1.704	臭氧胁迫响应相关蛋白质 Ozone-responsive stress-related protein-like
Q7XI37	LOC_Os07g42890	1.918	1.150	1.668	可能的FH互作蛋白质FIP1 Putative FH protein interacting protein FIP1
Q69MT6	LOC_Os09g34140	1.908	1.158	1.649	假定蛋白质 Hypothetical protein OSJNBb0034B12.21
Q338P8	LOC_Os10g25010	1.570	1.013	1.550	可能的钙离子结合蛋白质 Probable calcium-binding protein CML8
Q8H8U1	LOC_Os03g17510	1.038	1.574	-1.517	可能的PPR重复蛋白质 Putative PPR repeat containing protein
Q7XI22	LOC_Os07g08880	1.215	1.877	-1.546	可能的ES43蛋白质 Putative ES43 protein
Q94GQ6	LOC_Os03g53620	1.129	1.959	-1.736	可能的脱落酸诱导蛋白质 Putative abscisic acid-inducible protein
功能未知蛋白质 Unknown-function protein
C7JA46	LOC_Os12g44380	1.792	-1.144	2.051
Q7XQ85	LOC_Os04g48850	-1.015	-1.894	1.864
Q0E0B4	LOC_Os02g35630	1.075	-1.548	1.664
Q7XVN6	LOC_Os04g32070	1.511	-1.055	1.594
Q0E3F2	LOC_Os02g08130	2.037	1.282	1.589
Q6YUH8	LOC_Os02g46956	1.174	1.848	-1.572
Q0DTK3	LOC_Os03g13850	-1.575	1.043	-1.642
Q6ES31	LOC_Os09g24710	1.018	1.757	-1.727
Q5N9C8	LOC_Os01g51220	-1.116	1.771	-1.976
Q0E225	LOC_Os02g17760	-2.793	-1.285	-2.179

蛋白质ID Protein ID	基因ID Gene ID	HT	HS	HT/HS	蛋白质的功能描述 Functional illustration of protein
能量代谢 Energy metabolism
Q9FU69	LOC_Os01g08090	-1.613	-2.899	1.800	糖基转移酶 Glycosyltransferase
Q7Y179	LOC_Os03g46660	1.794	1.000	1.793	葡聚糖-1,3-β-葡萄糖苷内切酶7 Glucan endo-1,3-beta-glucosidase 7
Q0IPF8	LOC_Os12g10570	-1.495	1.012	-1.513	ATP合成酶亚基β ATP synthase subunit beta
Q2R1N0	LOC_Os11g38650	-1.739	-1.085	-1.605	糖基转移酶 Glycosyltransferase
Q84T00	LOC_Os03g58800	-1.805	1.044	-1.887	ATP酶家族蛋白质 ATPase, AAA family protein
物质转运与代谢 Matter translocation and metabolism
Q8GTK2	LOC_Os07g46350	1.832	1.097	1.669	羧肽酶 Carboxypeptidase
Q7Y007	LOC_Os03g47940	2.076	1.330	1.561	类似GDSL脂肪酶或酰基水解酶家族蛋白质 GDSL-like Lipase/Acylhydrolase family protein
Q10BU2	LOC_Os03g58980	-1.672	1.267	-2.119	类似(种子)萌发蛋白质3-7 Germin-like protein 3-7
光合作用 Photosynthesis
Q2R237	LOC_Os11g37130	3.115	1.199	2.598	叶绿体独立转位酶蛋白质 TATB Sec-independent protein translocase protein TATB, chloroplastic
Q658I1	LOC_Os06g03790	-1.667	1.674	-2.793	核糖体蛋白质L29 Ribosomal protein L29
逆境应答 Stress response
Q67TK9	B1012G11.2	2.852	-1.041	2.967	锌指蛋白质 Zinc knuckle containing protein-like
Q10N88	LOC_Os03g17020	2.341	1.326	1.765	ZIGA2锌指蛋白质 ARFGAP-like zinc finger-containing protein ZIGA2
Q84Q72	LOC_Os03g16030	1.906	2.975	-1.560	18.1 kD热激蛋白质类型Ⅰ 18.1 kD class Ⅰ heat shock protein
Q5YLY5	LOC_Os06g50840	-4.167	-1.560	-2.667	锌指蛋白质 Zinc finger protein-like
推测性功能注释蛋白质 Preliminary annotation of unknown-function protein
Q6ZJ47	LOC_Os08g02550	3.150	-1.522	4.796	可能的COP9复合亚基3 Putative COP9 complex subunit 3, FUS11
Q6ZCC9	LOC_Os08g02030	1.842	-1.764	3.251	可能的AER蛋白质 Putative AER
Q6ZCP8	LOC_Os08g07060	2.046	-1.477	3.020	可能的泛素氧化还原酶亚基1 Putative ubiquinone oxidoreductase subunit 1
Q6I587	LOC_Os05g5081	2.045	1.057	1.934	可能的钙依赖蛋白质激酶 Putative calcium-dependent protein kinase
Q53M16	LOC_Os11g13690	1.109	-1.560	1.730	假定蛋白质 Hypothetical protein
Q9LWS6	LOC_Os06g02470	1.699	-1.003	1.704	臭氧胁迫响应相关蛋白质 Ozone-responsive stress-related protein-like
Q7XI37	LOC_Os07g42890	1.918	1.150	1.668	可能的FH互作蛋白质FIP1 Putative FH protein interacting protein FIP1
Q69MT6	LOC_Os09g34140	1.908	1.158	1.649	假定蛋白质 Hypothetical protein OSJNBb0034B12.21
Q338P8	LOC_Os10g25010	1.570	1.013	1.550	可能的钙离子结合蛋白质 Probable calcium-binding protein CML8
Q8H8U1	LOC_Os03g17510	1.038	1.574	-1.517	可能的PPR重复蛋白质 Putative PPR repeat containing protein
Q7XI22	LOC_Os07g08880	1.215	1.877	-1.546	可能的ES43蛋白质 Putative ES43 protein
Q94GQ6	LOC_Os03g53620	1.129	1.959	-1.736	可能的脱落酸诱导蛋白质 Putative abscisic acid-inducible protein
功能未知蛋白质 Unknown-function protein
C7JA46	LOC_Os12g44380	1.792	-1.144	2.051
Q7XQ85	LOC_Os04g48850	-1.015	-1.894	1.864
Q0E0B4	LOC_Os02g35630	1.075	-1.548	1.664
Q7XVN6	LOC_Os04g32070	1.511	-1.055	1.594
Q0E3F2	LOC_Os02g08130	2.037	1.282	1.589
Q6YUH8	LOC_Os02g46956	1.174	1.848	-1.572
Q0DTK3	LOC_Os03g13850	-1.575	1.043	-1.642
Q6ES31	LOC_Os09g24710	1.018	1.757	-1.727
Q5N9C8	LOC_Os01g51220	-1.116	1.771	-1.976
Q0E225	LOC_Os02g17760	-2.793	-1.285	-2.179