中国水稻科学 ›› 2016, Vol. 30 ›› Issue (3): 265-272.DOI: 10.16819/j.1001-7216.2016.5188
收稿日期:
2015-12-22
修回日期:
2016-01-08
出版日期:
2016-05-10
发布日期:
2016-05-10
通讯作者:
许明辉
作者简介:
# 共同第一作者;
基金资助:
Ji-qiong MA, Yi YANG, Yi-ding SUN, Yan-yan WANG, Ming-hui XU*()
Received:
2015-12-22
Revised:
2016-01-08
Online:
2016-05-10
Published:
2016-05-10
Contact:
Ming-hui XU
About author:
# These authors contributed equally to this work;
摘要:
稻瘟病抗性基因序列变异的分析与认识是挖掘水稻抗稻瘟病基因资源的基础。对来源于云南省56个县(市)的218份地方稻品种稻瘟病抗性基因Pita第二编码区功能位点区段的609个碱基进行了测序分析。结果表明,所有供试品种在此序列上有18个变异位点,存在46种DNA单倍型和28种蛋白类型,其中DNA单倍型Pita-H1、Pita-H2、 Pita-H6频率较高,三者比例分别为17.89%、29.82%、20.18%,合计67.89%,是云南的优势单倍型,而其他单倍型频率较低,频率0.46%~3.67%。携带Pita抗性基因(编码区第2752位功能位点碱基为G)的品种共61个,占供试材料的27.98%,其中39个品种(占63.93%)与越南抗性品种Tetep序列一致。Pita抗性基因在水稻各亚种和生态型中的分布是不平衡的,籼稻中的频率为35.06%,粳稻中为24.11%,水稻中31.03%,陆稻中21.92%,黏稻中30.77%,糯稻中18.37%。Pita抗性基因在云南省广泛分布,36县(市)检测到了Pita抗性基因,但南部地区频率高于其他地区,且有的县频率较高,呈小集中的特点,海拔2200m以上的7个品种未能检测到Pita抗性基因。
中图分类号:
马继琼, 杨奕, 孙一丁, 王炎炎, 许明辉. 云南地方水稻品种抗稻瘟病基因Pita功能区段序列变异分析[J]. 中国水稻科学, 2016, 30(3): 265-272.
Ji-qiong MA, Yi YANG, Yi-ding SUN, Yan-yan WANG, Ming-hui XU. Sequence Variation of Functional Fragment in Pita Gene for Rice Blast Resistance in Yunnan Rice Landraces[J]. Chinese Journal OF Rice Science, 2016, 30(3): 265-272.
单倍型 Haplotype | 品种数 No. of accessions | 比例 Percentage /% | 2184 | 2249 | 2283 | 2291 | 2354 | 2373 | 2388 | 2395 | 2402 | 2466 | 2477 | 2495 | 2575 | 2584 | 2715 | 2726 | 2752* | 2766 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Tetep | T | T | A | C | C | G | A | C | T | T | C | C | C | G | C | C | G | A | ||
日本晴Nipponbare | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | ||
H1 | 39 | 17.89 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
H2 | 65 | 29.82 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . |
H3 | 3 | 1.38 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | A | . | . |
H4 | 8 | 3.67 | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | . | . | |
H5 | 8 | 3.67 | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | T | . | |
H6 | 44 | 20.18 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | G |
H7 | 7 | 3.21 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | |
H8 | 1 | 0.46 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | A | . | . |
H9 | 1 | 0.46 | . | . | . | . | . | . | . | T | . | . | . | . | T | . | . | . | . | . |
H10 | 3 | 1.38 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | . | . | T | G |
H11 | 2 | 0.92 | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | A | T | G |
H12 | 1 | 0.46 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | T | . |
H13 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | G | A | . | . |
H14 | 1 | 0.46 | . | . | . | . | . | C | G | T | . | . | . | . | . | . | . | A | . | . |
H15 | 1 | 0.46 | C | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | T | G |
H16 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | . | A | T | . |
H17 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | . |
H18 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | A | . | . |
H19 | 1 | 0.46 | . | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | . | . |
H20 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | G | A | T | G |
H21 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | G |
H22 | 1 | 0.46 | C | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | . | . |
H23 | 1 | 0.46 | C | . | . | . | . | . | G | T | C | . | . | . | T | . | . | . | . | . |
H24 | 1 | 0.46 | . | . | . | . | . | . | . | T | . | . | . | . | T | . | . | . | T | . |
H25 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | . |
H26 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | . | . | . | . | T | G |
H27 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | G | . | T | G |
H28 | 1 | 0.46 | . | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | T | . |
H29 | 2 | 0.92 | C | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | T | . |
H30 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | A | T | G |
H31 | 1 | 0.46 | . | C | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | G |
H32 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | . | . | G | . | T | G |
H33 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | G |
H34 | 1 | 0.46 | C | C | . | . | . | . | G | T | . | C | . | . | T | . | . | . | . | . |
H35 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | T | . | . | A | T | . |
H36 | 1 | 0.46 | C | C | . | . | . | . | G | T | . | . | . | . | T | C | . | . | T | . |
H37 | 2 | 0.92 | C | . | . | . | . | C | G | T | C | . | . | . | T | C | . | . | T | . |
H38 | 1 | 0.46 | C | . | . | T | . | C | G | T | . | . | . | . | T | . | . | . | T | G |
H39 | 1 | 0.46 | C | C | . | . | . | C | G | T | . | C | . | . | T | . | . | . | T | G |
H40 | 1 | 0.46 | C | . | . | T | . | C | G | T | . | C | . | . | T | C | . | . | T | G |
H41 | 1 | 0.46 | C | . | T | . | T | C | G | T | . | . | G | . | T | . | . | . | T | G |
H42 | 1 | 0.46 | C | . | . | . | . | C | G | T | . | C | G | A | T | C | . | A | . | . |
H43 | 1 | 0.46 | C | C | . | . | . | C | G | T | . | C | G | A | T | . | G | A | . | . |
H44 | 1 | 0.46 | C | C | . | . | . | C | G | T | C | C | G | A | T | C | . | . | T | . |
H45 | 1 | 0.46 | C | . | T | T | T | C | G | T | C | C | . | A | T | C | . | . | T | . |
H46 | 1 | 0.46 | C | C | T | T | T | C | G | T | C | C | G | . | T | . | . | . | T | G |
合计 | 218 | 100 |
表1 云南水稻地方品种Pita基因功能序列变异位点与单倍型
Table 1 Sequence variant loci and haplotypes of Pita gene in rice landraces from Yunnan.
单倍型 Haplotype | 品种数 No. of accessions | 比例 Percentage /% | 2184 | 2249 | 2283 | 2291 | 2354 | 2373 | 2388 | 2395 | 2402 | 2466 | 2477 | 2495 | 2575 | 2584 | 2715 | 2726 | 2752* | 2766 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Tetep | T | T | A | C | C | G | A | C | T | T | C | C | C | G | C | C | G | A | ||
日本晴Nipponbare | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | ||
H1 | 39 | 17.89 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
H2 | 65 | 29.82 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . |
H3 | 3 | 1.38 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | A | . | . |
H4 | 8 | 3.67 | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | . | . | |
H5 | 8 | 3.67 | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | T | . | |
H6 | 44 | 20.18 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | G |
H7 | 7 | 3.21 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | |
H8 | 1 | 0.46 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | A | . | . |
H9 | 1 | 0.46 | . | . | . | . | . | . | . | T | . | . | . | . | T | . | . | . | . | . |
H10 | 3 | 1.38 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | . | . | T | G |
H11 | 2 | 0.92 | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | A | T | G |
H12 | 1 | 0.46 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | T | . |
H13 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | G | A | . | . |
H14 | 1 | 0.46 | . | . | . | . | . | C | G | T | . | . | . | . | . | . | . | A | . | . |
H15 | 1 | 0.46 | C | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | T | G |
H16 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | . | A | T | . |
H17 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | . |
H18 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | A | . | . |
H19 | 1 | 0.46 | . | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | . | . |
H20 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | G | A | T | G |
H21 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | G |
H22 | 1 | 0.46 | C | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | . | . |
H23 | 1 | 0.46 | C | . | . | . | . | . | G | T | C | . | . | . | T | . | . | . | . | . |
H24 | 1 | 0.46 | . | . | . | . | . | . | . | T | . | . | . | . | T | . | . | . | T | . |
H25 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | . |
H26 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | . | . | . | . | T | G |
H27 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | . | . | G | . | T | G |
H28 | 1 | 0.46 | . | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | T | . |
H29 | 2 | 0.92 | C | . | . | . | . | C | G | T | . | . | . | . | T | . | . | . | T | . |
H30 | 1 | 0.46 | . | . | . | . | . | . | G | T | . | . | . | . | T | . | . | A | T | G |
H31 | 1 | 0.46 | . | C | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | G |
H32 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | . | . | G | . | T | G |
H33 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | T | . | . | . | T | G |
H34 | 1 | 0.46 | C | C | . | . | . | . | G | T | . | C | . | . | T | . | . | . | . | . |
H35 | 1 | 0.46 | C | . | . | . | . | . | G | T | . | . | . | . | T | . | . | A | T | . |
H36 | 1 | 0.46 | C | C | . | . | . | . | G | T | . | . | . | . | T | C | . | . | T | . |
H37 | 2 | 0.92 | C | . | . | . | . | C | G | T | C | . | . | . | T | C | . | . | T | . |
H38 | 1 | 0.46 | C | . | . | T | . | C | G | T | . | . | . | . | T | . | . | . | T | G |
H39 | 1 | 0.46 | C | C | . | . | . | C | G | T | . | C | . | . | T | . | . | . | T | G |
H40 | 1 | 0.46 | C | . | . | T | . | C | G | T | . | C | . | . | T | C | . | . | T | G |
H41 | 1 | 0.46 | C | . | T | . | T | C | G | T | . | . | G | . | T | . | . | . | T | G |
H42 | 1 | 0.46 | C | . | . | . | . | C | G | T | . | C | G | A | T | C | . | A | . | . |
H43 | 1 | 0.46 | C | C | . | . | . | C | G | T | . | C | G | A | T | . | G | A | . | . |
H44 | 1 | 0.46 | C | C | . | . | . | C | G | T | C | C | G | A | T | C | . | . | T | . |
H45 | 1 | 0.46 | C | . | T | T | T | C | G | T | C | C | . | A | T | C | . | . | T | . |
H46 | 1 | 0.46 | C | C | T | T | T | C | G | T | C | C | G | . | T | . | . | . | T | G |
合计 | 218 | 100 |
蛋白类型 Protein type | DNA单倍型 Haplotypes | 750 | 761 | 764 | 785 | 791 | 801 | 826 | 832 | 859 | 862 | 905 | 909 | 918* |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Tetep | L | R | A | T | M | F | S | T | L | D | S | S | A | |
日本晴Nipponbare | . | . | . | . | . | . | . | . | . | . | . | . | S | |
Pita-p1 | Pita-H1, Pita-H4 | . | . | . | . | . | . | . | . | . | . | . | . | . |
Pita-p2 | Pita-H2, Pita-H5, Pita-H 6, Pita-H7, Pita-H10, Pita-H15, Pita-H26 | . | . | . | . | . | . | . | . | . | . | . | . | S |
Pita-p3 | Pita-H3 | . | . | . | . | . | . | . | . | . | . | . | Y | . |
Pita-p4 | Pita-H9 | . | . | . | . | . | . | . | . | F | . | . | . | . |
Pita-p5 | Pita-H11, Pita-H16 | . | . | . | . | . | . | . | . | . | . | . | Y | S |
Pita-p6 | Pita-H8, Pita-H13 | . | . | . | . | . | . | . | . | . | . | R | Y | . |
Pita-p7 | Pita-H12, Pita-H27, Pita-H32 | . | . | . | . | . | . | . | . | . | . | R | . | S |
Pita-p8 | Pita-H17, Pita-H21, Pita-H24, Pita-H25, Pita-H33 | . | . | . | . | . | . | . | . | F | . | . | . | S |
Pita-p9 | Pita-H18 | . | . | . | . | . | . | . | . | F | . | . | Y | . |
Pita-p10 | Pita-H23 | . | . | . | . | . | S | . | . | F | . | . | . | . |
Pita-p11 | Pita-H14 | . | . | . | . | I | . | . | . | . | . | . | Y | . |
Pita-p12 | Pita-H19, Pita-H22 | . | . | . | . | I | . | . | . | F | . | . | . | . |
Pita-p13 | Pita-H34 | P | . | . | . | . | . | . | . | F | . | . | . | . |
Pita-p14 | Pita-H20 | . | . | . | . | . | . | . | T | . | . | R | Y | S |
Pita-p15 | Pita-H30, Pita-H35 | . | . | . | . | . | . | . | T | F | . | . | Y | S |
Pita-p16 | Pita-H28, Pita-H29 | . | . | . | . | I | . | . | T | F | . | . | . | S |
Pita-p17 | Pita-H31 | P | . | . | . | . | . | . | T | F | . | . | . | S |
Pita-p18 | Pita-H36 | P | . | . | . | . | . | . | T | F | H | . | . | S |
Pita-p19 | Pita-H39 | P | . | . | . | I | . | . | T | F | . | . | . | S |
Pita-p20 | Pita-H38 | . | . | V | . | I | . | . | T | F | . | . | . | S |
Pita-p21 | Pita-H37 | . | . | . | . | I | S | . | T | F | H | . | . | S |
Pita-p22 | Pita-H40 | . | . | V | . | I | . | . | T | F | H | . | . | S |
Pita-p23 | Pita-H41 | . | S | . | I | I | . | C | T | F | . | . | . | S |
Pita-p24 | Pita-H42 | . | . | . | . | I | . | C | N | F | H | . | Y | . |
Pita-p25 | Pita-H43 | P | . | . | . | I | . | C | N | F | . | R | Y | . |
Pita-p26 | Pita-H44 | P | . | . | . | I | S | C | N | F | H | . | . | S |
Pita-p27 | Pita-H45 | . | S | V | I | I | S | . | N | F | H | . | . | S |
Pita-p28 | Pita-H46 | P | S | V | I | I | S | C | T | F | . | . | . | S |
表2 云南水稻地方品种Pita基因编码蛋白序列变异位点与蛋白质类型
Table 2 Sequence variant loci and type of Pita gene encoded proteins in rice landraces from Yunnan.
蛋白类型 Protein type | DNA单倍型 Haplotypes | 750 | 761 | 764 | 785 | 791 | 801 | 826 | 832 | 859 | 862 | 905 | 909 | 918* |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Tetep | L | R | A | T | M | F | S | T | L | D | S | S | A | |
日本晴Nipponbare | . | . | . | . | . | . | . | . | . | . | . | . | S | |
Pita-p1 | Pita-H1, Pita-H4 | . | . | . | . | . | . | . | . | . | . | . | . | . |
Pita-p2 | Pita-H2, Pita-H5, Pita-H 6, Pita-H7, Pita-H10, Pita-H15, Pita-H26 | . | . | . | . | . | . | . | . | . | . | . | . | S |
Pita-p3 | Pita-H3 | . | . | . | . | . | . | . | . | . | . | . | Y | . |
Pita-p4 | Pita-H9 | . | . | . | . | . | . | . | . | F | . | . | . | . |
Pita-p5 | Pita-H11, Pita-H16 | . | . | . | . | . | . | . | . | . | . | . | Y | S |
Pita-p6 | Pita-H8, Pita-H13 | . | . | . | . | . | . | . | . | . | . | R | Y | . |
Pita-p7 | Pita-H12, Pita-H27, Pita-H32 | . | . | . | . | . | . | . | . | . | . | R | . | S |
Pita-p8 | Pita-H17, Pita-H21, Pita-H24, Pita-H25, Pita-H33 | . | . | . | . | . | . | . | . | F | . | . | . | S |
Pita-p9 | Pita-H18 | . | . | . | . | . | . | . | . | F | . | . | Y | . |
Pita-p10 | Pita-H23 | . | . | . | . | . | S | . | . | F | . | . | . | . |
Pita-p11 | Pita-H14 | . | . | . | . | I | . | . | . | . | . | . | Y | . |
Pita-p12 | Pita-H19, Pita-H22 | . | . | . | . | I | . | . | . | F | . | . | . | . |
Pita-p13 | Pita-H34 | P | . | . | . | . | . | . | . | F | . | . | . | . |
Pita-p14 | Pita-H20 | . | . | . | . | . | . | . | T | . | . | R | Y | S |
Pita-p15 | Pita-H30, Pita-H35 | . | . | . | . | . | . | . | T | F | . | . | Y | S |
Pita-p16 | Pita-H28, Pita-H29 | . | . | . | . | I | . | . | T | F | . | . | . | S |
Pita-p17 | Pita-H31 | P | . | . | . | . | . | . | T | F | . | . | . | S |
Pita-p18 | Pita-H36 | P | . | . | . | . | . | . | T | F | H | . | . | S |
Pita-p19 | Pita-H39 | P | . | . | . | I | . | . | T | F | . | . | . | S |
Pita-p20 | Pita-H38 | . | . | V | . | I | . | . | T | F | . | . | . | S |
Pita-p21 | Pita-H37 | . | . | . | . | I | S | . | T | F | H | . | . | S |
Pita-p22 | Pita-H40 | . | . | V | . | I | . | . | T | F | H | . | . | S |
Pita-p23 | Pita-H41 | . | S | . | I | I | . | C | T | F | . | . | . | S |
Pita-p24 | Pita-H42 | . | . | . | . | I | . | C | N | F | H | . | Y | . |
Pita-p25 | Pita-H43 | P | . | . | . | I | . | C | N | F | . | R | Y | . |
Pita-p26 | Pita-H44 | P | . | . | . | I | S | C | N | F | H | . | . | S |
Pita-p27 | Pita-H45 | . | S | V | I | I | S | . | N | F | H | . | . | S |
Pita-p28 | Pita-H46 | P | S | V | I | I | S | C | T | F | . | . | . | S |
图1 云南地方水稻品种Pita基因功能片段编码的29种蛋白单倍型系统进化关系 *抗性单倍型。
Fig. 1. Phylogenetic tree constructed with the 29 protein haplotypes encoded by Pita genes from Yunnan rice landraces. * Indicates the resistant haplotypes.
群体类型 Group type | 材料数 No. of accessions | 携带G样品数 No. of varieties carrying G locus | 频率 Frequency/% |
---|---|---|---|
总体Total population | 218 | 61 | 27.98 |
籼稻indica | 77 | 27 | 35.06 |
粳稻japonica | 141 | 34 | 24.11 |
水稻Rice | 145 | 45 | 31.03 |
陆稻Upland rice | 73 | 16 | 21.92 |
黏稻Non-glutinous rice | 169 | 52 | 30.77 |
糯稻Glutinous rice | 49 | 9 | 18.37 |
表3 Pita抗性基因在云南地方水稻品种各亚种和生态类型中的分布
Table 3 Distribution of Pita resistance genes in different subspecies and ecotypes.
群体类型 Group type | 材料数 No. of accessions | 携带G样品数 No. of varieties carrying G locus | 频率 Frequency/% |
---|---|---|---|
总体Total population | 218 | 61 | 27.98 |
籼稻indica | 77 | 27 | 35.06 |
粳稻japonica | 141 | 34 | 24.11 |
水稻Rice | 145 | 45 | 31.03 |
陆稻Upland rice | 73 | 16 | 21.92 |
黏稻Non-glutinous rice | 169 | 52 | 30.77 |
糯稻Glutinous rice | 49 | 9 | 18.37 |
地点 Location | 材料数 No. of accessions | 抗性基因数 No. of resistance genes | 频率 Frequency /% | 地点 Location | 材料数 No. of accessions | 抗性基因数 No. of resistance genes | 频率 Frequency /% |
---|---|---|---|---|---|---|---|
西双版纳州Xishuangbanna | 21 | 8 | 38.10 | 保山市Baoshan | 14 | 5 | 35.58 |
勐腊县 Mengla | 2 | 0 | 腾冲县 Tengchong | 7 | 2 | ||
勐海县 Menghai | 11 | 2 | 昌宁县 Changning | 2 | 1 | ||
景洪市Jinghong | 8 | 3 | 龙陵县 Longling | 2 | 1 | ||
普洱市Pu'er | 52 | 17 | 32.69 | 隆阳区Longyang | 3 | 1 | |
孟连县 Menglian | 9 | 3 | 玉溪市 Yuxi | 13 | 3 | 23.08 | |
澜沧县 Lancang | 8 | 1 | 新平县 Xinping | 8 | 2 | ||
西盟县 Ximeng | 5 | 3 | 峨山县 Eshan | 2 | 1 | ||
江城县 Jiangcheng | 5 | 1 | 江川县 Jiangchuan | 2 | 0 | ||
宁洱县 Ning’er | 3 | 1 | 易门县 Yimen | 1 | 0 | ||
墨江县 Mojiang | 21 | 8 | 昆明市Kunming | 2 | 1 | 50.00 | |
景东县 Jingdong | 1 | 0 | 富民县 Fumin | 2 | 1 | ||
红河州 Honghe | 16 | 6 | 37.50 | 曲靖市Qujing | 3 | 1 | 33.33 |
金平县 Jinping | 4 | 2 | 富源县 Fuyuan | 1 | 0 | ||
屏边县 Pingbian | 7 | 2 | 师宗县 Shizong | 1 | 0 | ||
红河县 Honghe | 1 | 1 | 麒麟区Qilin | 1 | 1 | ||
开远市Kaiyuan | 3 | 1 | 昭通市Zhaotong | 13 | 4 | 30.77 | |
弥勒县 Mile | 1 | 0 | 巧家县 Qiaojia | 1 | 0 | ||
文山州Wenshan | 12 | 5 | 41.67 | 镇雄县 Zhenxiong | 1 | 0 | |
富宁县 Funing | 1 | 0 | 彝良县 Yiliang | 2 | 1 | ||
文山县 Wenshan | 5 | 2 | 大关县 Daguan | 3 | 0 | ||
广南县 Guangnan | 6 | 3 | 盐津县 Yanjin | 3 | 2 | ||
临沧市Lincang | 36 | 6 | 16.67 | 威信县 Weixin | 3 | 1 | |
沧源县 Cangyuan | 2 | 0 | 大理市Dali | 3 | 1 | 33.33 | |
双江县 Shuangjiang | 2 | 1 | 大理市Dali | 1 | 1 | ||
耿马县 Gengma | 12 | 2 | 剑川县 Jianchuan | 1 | 0 | ||
镇康县 Zhenkang | 4 | 1 | 云龙县 Yunlong | 1 | 0 | ||
临翔区Linxiang | 1 | 0 | 丽江市 Lijiang | 12 | 3 | 25.00 | |
永德县 Yongde | 10 | 1 | 永胜县 Yongsheng | 3 | 3 | ||
云县 Yunxian | 3 | 1 | 宁蒗县 Ninglang | 3 | 0 | ||
凤庆县 Fengqing | 2 | 0 | 玉龙县 Yulong | 3 | 0 | ||
德宏州Dehong | 20 | 4 | 20.00 | 华坪县 Huaping | 3 | 0 | |
瑞丽县 Ruili | 6 | 1 | 迪庆州Diqing | 1 | 0 | 0 | |
芒市Mangshi | 2 | 0 | 维西县 Weixi | 1 | 0 | ||
陇川县 Longchuan | 2 | 0 | 总计 Total | 218 | 61 | 27.98 | |
盈江县 Yingjiang | 5 | 2 | |||||
梁河县 Lianghe | 5 | 1 |
表4 Pita抗性基因在云南不同地区和县(市)的分布
Table 4 Distribution of Pita resistance genes in Yunnan different regions.
地点 Location | 材料数 No. of accessions | 抗性基因数 No. of resistance genes | 频率 Frequency /% | 地点 Location | 材料数 No. of accessions | 抗性基因数 No. of resistance genes | 频率 Frequency /% |
---|---|---|---|---|---|---|---|
西双版纳州Xishuangbanna | 21 | 8 | 38.10 | 保山市Baoshan | 14 | 5 | 35.58 |
勐腊县 Mengla | 2 | 0 | 腾冲县 Tengchong | 7 | 2 | ||
勐海县 Menghai | 11 | 2 | 昌宁县 Changning | 2 | 1 | ||
景洪市Jinghong | 8 | 3 | 龙陵县 Longling | 2 | 1 | ||
普洱市Pu'er | 52 | 17 | 32.69 | 隆阳区Longyang | 3 | 1 | |
孟连县 Menglian | 9 | 3 | 玉溪市 Yuxi | 13 | 3 | 23.08 | |
澜沧县 Lancang | 8 | 1 | 新平县 Xinping | 8 | 2 | ||
西盟县 Ximeng | 5 | 3 | 峨山县 Eshan | 2 | 1 | ||
江城县 Jiangcheng | 5 | 1 | 江川县 Jiangchuan | 2 | 0 | ||
宁洱县 Ning’er | 3 | 1 | 易门县 Yimen | 1 | 0 | ||
墨江县 Mojiang | 21 | 8 | 昆明市Kunming | 2 | 1 | 50.00 | |
景东县 Jingdong | 1 | 0 | 富民县 Fumin | 2 | 1 | ||
红河州 Honghe | 16 | 6 | 37.50 | 曲靖市Qujing | 3 | 1 | 33.33 |
金平县 Jinping | 4 | 2 | 富源县 Fuyuan | 1 | 0 | ||
屏边县 Pingbian | 7 | 2 | 师宗县 Shizong | 1 | 0 | ||
红河县 Honghe | 1 | 1 | 麒麟区Qilin | 1 | 1 | ||
开远市Kaiyuan | 3 | 1 | 昭通市Zhaotong | 13 | 4 | 30.77 | |
弥勒县 Mile | 1 | 0 | 巧家县 Qiaojia | 1 | 0 | ||
文山州Wenshan | 12 | 5 | 41.67 | 镇雄县 Zhenxiong | 1 | 0 | |
富宁县 Funing | 1 | 0 | 彝良县 Yiliang | 2 | 1 | ||
文山县 Wenshan | 5 | 2 | 大关县 Daguan | 3 | 0 | ||
广南县 Guangnan | 6 | 3 | 盐津县 Yanjin | 3 | 2 | ||
临沧市Lincang | 36 | 6 | 16.67 | 威信县 Weixin | 3 | 1 | |
沧源县 Cangyuan | 2 | 0 | 大理市Dali | 3 | 1 | 33.33 | |
双江县 Shuangjiang | 2 | 1 | 大理市Dali | 1 | 1 | ||
耿马县 Gengma | 12 | 2 | 剑川县 Jianchuan | 1 | 0 | ||
镇康县 Zhenkang | 4 | 1 | 云龙县 Yunlong | 1 | 0 | ||
临翔区Linxiang | 1 | 0 | 丽江市 Lijiang | 12 | 3 | 25.00 | |
永德县 Yongde | 10 | 1 | 永胜县 Yongsheng | 3 | 3 | ||
云县 Yunxian | 3 | 1 | 宁蒗县 Ninglang | 3 | 0 | ||
凤庆县 Fengqing | 2 | 0 | 玉龙县 Yulong | 3 | 0 | ||
德宏州Dehong | 20 | 4 | 20.00 | 华坪县 Huaping | 3 | 0 | |
瑞丽县 Ruili | 6 | 1 | 迪庆州Diqing | 1 | 0 | 0 | |
芒市Mangshi | 2 | 0 | 维西县 Weixi | 1 | 0 | ||
陇川县 Longchuan | 2 | 0 | 总计 Total | 218 | 61 | 27.98 | |
盈江县 Yingjiang | 5 | 2 | |||||
梁河县 Lianghe | 5 | 1 |
海拔 Elevation/m | 材料数 No. of accessions | 抗性基因数 No. of resistance genes | 比例 Percentage /% |
---|---|---|---|
<800 | 20 | 6 | 30.0 |
801-1200 | 85 | 20 | 23.5 |
1201-1600 | 73 | 23 | 31.5 |
1601-2000 | 28 | 10 | 35.7 |
2001-2200 | 5 | 3 | 60.0 |
>2200 | 7 | 0 | 0.0 |
表5 Pita抗性基因在云南不同海拔地区的分布
Table 5 Distribution of Pita resistance genes at different elevation in Yunnan.
海拔 Elevation/m | 材料数 No. of accessions | 抗性基因数 No. of resistance genes | 比例 Percentage /% |
---|---|---|---|
<800 | 20 | 6 | 30.0 |
801-1200 | 85 | 20 | 23.5 |
1201-1600 | 73 | 23 | 31.5 |
1601-2000 | 28 | 10 | 35.7 |
2001-2200 | 5 | 3 | 60.0 |
>2200 | 7 | 0 | 0.0 |
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