水稻新裂颖突变体sg1的遗传分析与基因定位

doi:10.3969/j.issn.1001-7216.2015.01.003

摘要/Abstract

摘要：

从中籼3037辐射突变体库中获得一个颖花开裂突变体(split glume1, sg1),表型分析发现该突变体不仅颖壳异常,且伴随着生育期提前,植株变矮,颖花退化等现象,结实率也显著降低。遗传分析和分子生物学分析结果表明该突变受1对隐性核基因控制,并最终将其定位在水稻第1染色体上分子标记M1-z21与M1-z27之间,物理距离约为47 kb,这是一种控制颖花发育的新基因,通过预测发现该区段内存在6个开放阅读框。

关键词: 水稻, 花器官, 颖花开裂突变体, 精细定位

Abstract:

A split glume mutant is from the mutant library of indica variety Zhongxian 3037 radiated by ⁶⁰Co γ-ray. Compared to its wild type, the mutant has not only split glumes, but also characterized by early heading, lower plant height, reduced seed setting rate and degenerated spikelets, which implies that the mutation has a pleiotropic effect. Genetic and molecular analysis indicated that the mutant was controlled by a recessive nuclear gene, temporally designated as split glume 1 (sg1), and then SG1 was fine mapped to a 47-kb DNA region between the STS markers M1-z21 and M1-z27 on 1, where six ORFs in total were predicted. On the basis of the location of SG1, it is deduced that SG1 is a novel gene regulating the rice glume development.

Key words: rice, flower organ, sg1, fine mapping

中图分类号:

Q343.5
Q755

曾生元, 郭旻, 李荣德, 盛生兰, 龚红兵, 严长杰. 水稻新裂颖突变体sg1的遗传分析与基因定位[J]. 中国水稻科学, 2015, 29(1): 22-27.

Sheng-yuan ZENG, Min GUO, Rong-de LI, Sheng-lan SHENG, Hong-bin GONG, Chang-jie YAN. Genetic Analysis and Fine Mapping for a Novel split glume Mutant in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(1): 22-27.

图/表 5

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性状 Trait	中籼3037 Zhongxian 3037 (Mean±SD)	突变体 sg1 (Mean±SD)	P值 P-value
株高 Plant height/cm	112.7±1.8	85.2±3.4	0.000
穗长 Panicle length/cm	24.4±0.5	23.8±1.3	0.504
每穗粒数 Grain number per panicle	227.6±40.1	145.7±19.3	0.033
结实率 Seed setting rate/%	89.0±0.2	28.0±0.9	0.000

性状 Trait	中籼3037 Zhongxian 3037 (Mean±SD)	突变体 sg1 (Mean±SD)	P值 P-value
株高 Plant height/cm	112.7±1.8	85.2±3.4	0.000
穗长 Panicle length/cm	24.4±0.5	23.8±1.3	0.504
每穗粒数 Grain number per panicle	227.6±40.1	145.7±19.3	0.033
结实率 Seed setting rate/%	89.0±0.2	28.0±0.9	0.000

组合 Combination	F₁	F₂
组合 Combination	F₁	正常表型株数 Normal plants	突变表型株数 Individuals with mutant type	χ²(3:1)
中花11/sg1 Zhonghua 11/sg1	正常 Normal	1705	95	373.4
sg1/越光 sg1/Koshihikari	正常 Normal	789	61	144.0

组合 Combination	F₁	F₂
组合 Combination	F₁	正常表型株数 Normal plants	突变表型株数 Individuals with mutant type	χ²(3:1)
中花11/sg1 Zhonghua 11/sg1	正常 Normal	1705	95	373.4
sg1/越光 sg1/Koshihikari	正常 Normal	789	61	144.0

标记名称 Name	正向引物序列(5’-3’) Forward primer sequence(5’-3’)	反向引物序列(5’-3’) Reverse primer sequence(5’-3’)	产物所在位置 Position	所在克隆 PAC/BAC
d33	TTCACTACAGATACCATAAACC	CTCAGTCACACTTCATCCCTA	12 842-13 102	AP003295
M1-z3	CGTTTGCCGTCCTGTTC	CCGAGGTCTGCCTGATTTA	74 824-74 943	AP003265
M1-z20	TATAGACGTTTCACTGATGG	GGTGGAAGTCAAGAATAATG	34 968-35 112	AP003265
M1-z21	ATCATCATCACGGTTTCA	ACCTGTTCCTTGCCTCA	15 269-15 396	AP003265
M1-z27	CAATCAAGCCCATAACTAC	TGGGTAGTTACACGAGATC	62 113-62 229	AP003265
M1-z28	GGAGCAGCACAAGAAGG	CCAGTTGAGTTGGAGGG	48 595-48 718	AP003265