水稻C2C2锌指蛋白基因OsLSD2对日本晴品种生长及氮素利用的影响

doi:10.3969/j.issn.1001-7216.2014.04.013

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (4): 435-441.DOI: 10.3969/j.issn.1001-7216.2014.04.013

水稻C2C2锌指蛋白基因OsLSD2对日本晴品种生长及氮素利用的影响

童君^#,陈景光^#,朱静雯,范晓荣^* ,徐国华

南京农业大学资源与环境科学学院，南京 210095；

收稿日期:2013-09-25 修回日期:2014-03-03 出版日期:2014-07-10 发布日期:2014-07-10
通讯作者: 范晓荣* ,
基金资助:
国家自然科学基金资助项目(NSFC 31172013)；国家转基因专项(2009ZX08009126B)；中央高校基本科研业务费自主创新重点研究项目(2050205)。

Effects of Rice C2C2 Zinc Finger Protein Gene OsLSD2 on the Growth and Nitrogen Utilization of Nipponbare

TONG Jun^#， CHEN Jingguang^#， ZHU Jingwen， FAN Xiaorong^*， XU Guohua

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China；

Received:2013-09-25 Revised:2014-03-03 Online:2014-07-10 Published:2014-07-10
Contact: FAN Xiaorong*，

摘要/Abstract

摘要： LSD1基因家族是一类特殊的C2C2型锌指蛋白基因。 OsLSD2 是LSD1 型基因家族的一员。已知LSD1基因参与了植物抗病信号途径。为了培育环境友好型水稻，我们研究了OsLSD2在日本晴中的过量表达。T3代转基因水稻表型表现为株高降低，分蘖数平均减少2个，结实率只有64%~72%，单株产量下降33%~42%，生育期提前15 d，对氮素的吸收利用率降低等，说明该基因过量表达显著抑制水稻的生长和氮素转运。

关键词: 水稻, LSD1型锌指蛋白基因, 过量表达, 氮素转运

Abstract: OsLSD2 is a member of LSD1 gene family， which encodes C2C2 zinc finger protein gene. As it is known that LSD1 family is involved in disease resistance. In order to create environmentfriendly rice plant, we investigated the effects of overexpression of this gene in Nipponbare (WT) on plant growth and nitrogen use efficiency. The T3 generation plants showed that the plant height was reduced significantly；tiller number was averagely decreased by two; seed setting rate was only 64%-72%; the grain yield was reduced by 33%-42% compared with WT; the growth duration was shortened by 15 days; and the absorption and utilization rate of nitrogen were reduced significantly as OsLSD2 overexpressed. The phenotype of transformed lines suggested the overexpression of OsLSD2 can significantly repress the growth and nitrogen translocation in rice.

Key words: rice, LSD1type zinc finger protein gene, overexpression； nitrogen translocation

中图分类号:

童君#,陈景光#,朱静雯,范晓荣* ,徐国华. 水稻C2C2锌指蛋白基因OsLSD2对日本晴品种生长及氮素利用的影响[J]. 中国水稻科学, 2014, 28(4): 435-441.

TONG Jun#， CHEN Jingguang#， ZHU Jingwen， FAN Xiaorong*， XU Guohua. Effects of Rice C2C2 Zinc Finger Protein Gene OsLSD2 on the Growth and Nitrogen Utilization of Nipponbare[J]. Chinese Journal of Rice Science, 2014, 28(4): 435-441.

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水稻C2C2锌指蛋白基因OsLSD2对日本晴品种生长及氮素利用的影响

Effects of Rice C2C2 Zinc Finger Protein Gene OsLSD2 on the Growth and Nitrogen Utilization of Nipponbare

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