杂交籼稻骨干保持系岗46B稻谷粒形及垩白的QTL分析

doi:10.3969/j.issn.1001-7216.2014.03.003

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (3): 235-242.DOI: 10.3969/j.issn.1001-7216.2014.03.003

杂交籼稻骨干保持系岗46B稻谷粒形及垩白的QTL分析

高方远^1,# ,邱玲^1,2,# ,陆贤军¹,任鄄胜¹ ,吴贤婷¹ ,任光俊¹ ,曾礼华^2,*

¹四川省农业科学院作物研究所，成都 610066； ²四川师范大学，成都 610110；

收稿日期:2013-11-02 修回日期:2013-11-26 出版日期:2014-05-10 发布日期:2014-05-10
通讯作者: 曾礼华2,*
基金资助:
四川省青年科技创新研究团队专项计划资助项目（2011JTD0022）；四川省财政创新能力提升工程资助项目（2013GXJS005）；国家863计划资助项目（2012AA1011025）。

QTL Analysis on Grain Shape and Chalkiness of an Elite Maintainer Line Gang 46B in Hybrid Rice (Oryza sativa L.)

GAO Fangyuan ^1,# , QIU Ling ^1,2,# , LU Xianjun ¹, REN Juansheng ¹, WU Xianting ¹, REN Guangjun ¹, ZENG Lihua ^2,*

¹Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; ² Sichuan Normal University, Chengdu 610110, China;

Received:2013-11-02 Revised:2013-11-26 Online:2014-05-10 Published:2014-05-10
Contact: ZENG Lihua2,*

摘要/Abstract

摘要： 利用籼稻保持系骨干亲本岗46B与美国水稻品种Lemont 多次回交培育的低垩白、窄粒株系K1075与岗46B构建F2群体，对稻谷粒长、粒宽、长宽比、粒厚、百粒重和垩白粒率进行数量性状位点（QTL）分析。6个性状在F2群体中均呈正态连续分布，属于典型的数量性状。除粒长与百粒重间没有显著相关性外，其他性状间均存在显著的正相关或负相关。采用SSR标记构建遗传连锁图，通过复合区间作图法共检测到14个控制稻谷粒形和垩白的QTL，包括2个控制粒长的QTL（qGL2和qGL5），2个控制粒宽的QTL（qGW5和qGW8），5个控制长宽比的QTL（qLWR2、qLWR5、qLWR6、qLWR8和qLWR12），1个控制粒厚的QTL（qGT5），1个控制百粒重的QTL（qHGW5）和3个控制垩白粒率的QTL（qPGWC5、qPGWC6和qPGWC8）。在这些位点中，岗46B等位基因增加粒宽、粒厚、粒重和垩白粒率，减小粒长和长宽比。控制粒宽、粒厚、粒重、垩白粒率和长宽比的主效QTL均位于第5染色体RM17990-RM18004-RM18068区间，这一结果与仅选择极端表型单株，采用连锁及连锁不平衡方法（LD）获得的结果一致。

关键词: 水稻, 粒形, 垩白, 数量性状位点, 连锁及连锁不平衡

Abstract: To identify the genetic loci that control grain length, width, lengthwidth ratio, thickness, 100grain weight and the percentage of grains with chalkiness (GL, GW, LWR, GT, HGW, PGWC), we constructed an F2 population by crossing an elite indica maintainer line Gang 46B(G46B) with K1075, an introgression line of G46B derived from multibackcross with an US rice variety Lemont, with low percentage of chalkiness and narrow grain. All six investigated traits followed a continuous phenotypic distribution. Significantly positive or negative correlations were observed between all six traits, except the GL and GW. A genetic map was constructed with 30 simple sequence repeat (SSR) markers and a total of 14 QTLs for grain shape and chalkiness were detected by composite interval mapping (CIM). Among which, two were for GL (qGL2 and qGL5), two for GW (qGW5 and qGW8), five for LWR (qLWR2, qLWR5, qLWR6, qLWR8 and qLWR12), one for GT (qGT5), one for HGW (qHGW5), and three for PGWC (qPGWC5, qPGWC6 and qPGWC8). The G46B alleles contributed to increasing GW, GT, HGW and PGWC, while decreasing GL and LWR at all detected loci. The major QTLs controlling GW, GT, GW, PGWC and LWR were located in the same region RM17990RM18004RM18068 on chromosome 5, which was correspondent to the results identified in extreme phenotype plants using linkage and linkage disequilibrium.

Key words: rice, grain shape, chalkiness, quantitative trait locus, linkage and linkage disequilibrium

中图分类号:

高方远1,#,邱玲1,2,# ,陆贤军1 ,任鄄胜1 ,吴贤婷1 ,任光俊1 ,曾礼华2,*. 杂交籼稻骨干保持系岗46B稻谷粒形及垩白的QTL分析[J]. 中国水稻科学, 2014, 28(3): 235-242.

GAO Fangyuan1,#, QIU Ling1,2,#, LU Xianjun1, REN Juansheng1, WU Xianting1, REN Guangjun1, ZENG Lihua2,*. QTL Analysis on Grain Shape and Chalkiness of an Elite Maintainer Line Gang 46B in Hybrid Rice (Oryza sativa L.)[J]. Chinese Journal of Rice Science, 2014, 28(3): 235-242.

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杂交籼稻骨干保持系岗46B稻谷粒形及垩白的QTL分析

QTL Analysis on Grain Shape and Chalkiness of an Elite Maintainer Line Gang 46B in Hybrid Rice (Oryza sativa L.)

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