褐飞虱体内细菌群落的PCRDGGE分析

doi:10.3969/j.issn.1001-7216.2014.02.015

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (2): 217-222.DOI: 10.3969/j.issn.1001-7216.2014.02.015

• 研究简报 • 上一篇

褐飞虱体内细菌群落的PCRDGGE分析

徐红星^1,2,郑许松²,杨亚军²,王新²,傅强³,叶恭银^1,* ,吕仲贤^2,*

¹浙江大学昆虫科学研究所, 杭州 310029；²浙江省农业科学院植物保护与微生物研究所, 杭州 310021; ³中国水稻研究所，杭州 310006；

收稿日期:2013-11-07 修回日期:2014-01-23 出版日期:2014-03-10 发布日期:2014-03-10
通讯作者: 叶恭银1,*,吕仲贤2,*
基金资助:
国家973计划资助项目（2010CB126202）；浙江省优先主题农业项目（2011C12022）。

PCRDGGE Analysis of the Bacterial Community in Different Populations of Rice Brown Planthopper, Nilaparvata lugens Stl

XU Hongxing ^1,2 , ZHENG Xusong ² , YANG Yajun ² , WANG Xin ² , FU Qiang ³ , YE Gongyin ^1*,LV Zhongxian ^2,*

¹ Institute of Insect Science, Zhejiang University Hangzhou 310058, China; ² Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; ³China National Rice Research Institute, Hangzhou 310006, China;

Received:2013-11-07 Revised:2014-01-23 Online:2014-03-10 Published:2014-03-10
Contact: YE Gongyin1*, LV Zhongxian2,*

摘要/Abstract

摘要： 应用变性梯度凝胶电泳（denatured gradient gel electrophoresis，DGGE）分析了褐飞虱体内细菌的群落结构。结果表明，褐飞虱1龄～5龄若虫体内细菌的菌群结构随其生长发育而异，而羽化后的成虫与1龄若虫的菌群结构具有较高的相似性。根据DGGE聚类分析结果，9个地理种群褐飞虱体内细菌可分为3类，菲律宾单独一组，泰国与我国的海南、云南和浙江种群为一组，越南与我国的广西、湖南和江西种群为一组。取食感虫品种的TN1种群褐飞虱体内细菌的群落结构相似，与取食抗虫品种的ASD7种群和Mudgo种群之间具有较明显的差异，但取食不同抗虫水稻品种的褐飞虱还未能得到有效区分。

关键词: 变性梯度凝胶电泳, 褐飞虱, 菌群结构, 地理种群, 致害性

Abstract: The bacterial community structure in different virulent populations of brown planthopper (BPH), Nilaparvata lugens (Stl), was analyzed by denatured gradient gel electrophoresis (DGGE). The results showed that the bacterial community in BPH nymph from the 1st to the 5th instars varied with nymphal growth and development, and the bacterial community in the 1st instar BPH nymph was similar to that in adults. Nine geographic BPH populations were obviously divided into three groups according to the cluster analysis based on DGGE fingerprint. The first group came from the Philippines, the second from Thailand and the provinces of Hainan, Yunnan and Zhejiang of China, and the third from Vietnam and the provinces of Guangxi, Hunan, and Jiangxi of China. BPH populations on Mudgo (with resistant gene Bph1) and ASD7 (with resistant gene bph2) have similar bacterial community, while differed to that fed on susceptible rice variety, TN1.

Key words: denatured gradient gel electrophoresis (DGGE), brown planthopper (BPH), Nilaparvata lugens, bacterial community, geographic population, virulent to resistant varieties

中图分类号:

徐红星1,2,郑许松2,杨亚军2,王新2,傅强3,叶恭银1,*,吕仲贤2,*. 褐飞虱体内细菌群落的PCRDGGE分析[J]. 中国水稻科学, 2014, 28(2): 217-222.

XU Hongxing1,2, ZHENG Xusong2, YANG Yajun2, WANG Xin2, FU Qiang3, YE Gongyin1*, . PCRDGGE Analysis of the Bacterial Community in Different Populations of Rice Brown Planthopper, Nilaparvata lugens Stl[J]. Chinese Journal of Rice Science, 2014, 28(2): 217-222.

参考文献

\[1\]程遐年, 吴进才, 马飞. 褐飞虱研究与防治. 北京: 中国农业出版社， 2003.

\[2\]程家安, 朱金良, 祝增荣，等. 稻田飞虱灾变与环境调控. 环境昆虫学报， 2008, 30(2): 176182.

\[3\]Savary S, Horgan F, Willocquet L, et al. A review of principles for sustainable pest management in rice. Crop Prot, 2012, 32: 5463.

\[4\]陆剑飞, 黄国洋. 浙江省褐飞虱暴发成灾的原因与治理对策. 农药科学与管理, 2006, 27(1): 4243.

\[5\]巫国瑞, 俞晓平, 陶林勇. 褐飞虱和白背飞虱灾害的长期预测. 中国农业科学, 1997, 30(4): 2529.

\[6\]翟保平.   稻飞虱: 国际视野下的中国问题. 应用昆虫学报, 2011, 48(5): 11841193.

\[7\]Lu Z X, Yu X P, Chen J M, et al.   Dynamics of yeastlike symbiote and its relationship with the virulence of brown planthopper, Nilaparvata lugens Stl, to resistant rice varieties. J AsiaPacific Entomol, 2004, 7(3): 317323.

\[8\]徐红星, 吕仲贤, 陈建明, 等. 褐飞虱在适应抗性水稻品种“IR26”过程中的氨基酸含量变化. 中国生态农业学报, 2008, 16(4): 925928.

\[9\]陈峰, 傅强, 罗举, 等. 苗期抗性不同的水稻品种成株期对褐飞虱的抗性. 中国水稻科学, 2009, 23(2): 201206.

\[10\]Horgan F. Mechanisms of resistance: A major gap in understanding planthopperrce interaction//Heong K L, Hardy B. Planthoppers: New Threats to the Sustainability of Intensive Rice Production Systems in Asia. Los Baos (Philippines): International Rice Research Institute, 2009： 281302.

\[11\]吕仲贤, 俞晓平, 陈建明, 等. 共生菌对褐飞虱生长发育和生殖的影响. 植物保护学报, 2001, 28(3): 193197.

\[12\]李青, 罗善昱, 韦素美, 等. 褐飞虱生物型测定及其与迁飞关系分析. 昆虫知识， 1999, 36(5): 257260.

\[13\]Moran N A. Symbiosis. Curr Biol， 2006, 16: 866871.

\[14\]Hosokawa T, Koga R, Kikuchi Y, et al. Wolbachia as a bacteriocyte associated nutritional mutualist. Proc Natl Acad Sci USA, 2009, 107(2): 769774.

\[15\]王国超, 傅强, 赖风香, 等. 褐飞虱体内类酵母共生菌与氨基酸营养的关系. 昆虫学报, 2005, 48(4): 483490.

\[16\]徐红星, 郑许松, 刘淑平, 等. 昆虫内共生菌在昆虫防御中的作用. 昆虫知识, 2009, 46(3): 350354.

\[17\]Feldhaar H, Gross R. Insects as hosts for Mutualistic bacteria. Int J Med Microbiol, 2009, 299: 18.

\[18\]张珏锋, 吕仲贤, 陈法军, 等. 褐飞虱不同致害性种群体内共生菌18S rDNA 部分序列比较. 昆虫学报, 2006, 49(3): 528532.

\[19\]Oliver K M, Moran N A, Hunter M S. Variation in resistance to parasitism in aphids is due to symbionts not host genotype. Proc Natl Acad Sci USA, 2005, 102: 1279512800.

\[20\]Lyte M, Gaykema R, Goehler L E. Behavior modification of host by microbes. BMC Biol, 2009, 7(32): 121127.

\[21\]Campbell B C. On the role of microbial symbiotes in herbivorous insect//Bernays E A. InsectPlant Interaction. Florida: CRC Press Inc, 1990：144.

\[22\]Simon J C, Carré S, Boutin M, et al. Host based divergence in populations of the pea aphid: Insights from nuclear markers and the prevalence of facultative symbionts. Proc R Soc London (B)， 2003, 270: 17031712.

\[23\]Tang M,Lv L, Jin S L, et al. Bacterial symbionts of the brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae). Appl Environ Microbiol， 2010, 76(6): 17401745.

\[24\]Fuhrman J A, Comeau D E, Hagstrom A, et al. Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies. Appl Environ Microbiol, 1988, 54: 14261429.

\[25\]Sandstrom J P, Russel J A, White J P, et al. Independment origins and horizontal transfer of bacterial symbionts in insects. Mol Ecol, 2001, 10: 217.

\[26\]王渭霞, 罗举, 赖凤香, 等. 水稻褐飞虱内生共生细菌Arsenophonus的鉴定和系统分析. 昆虫学报, 2010, 53(6): 647654.

\[27\]Muyzer G, Dewaal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reactionamplified genes coding for 16S rRNA. Appl Environ Microbiol, 1993, 59(3): 695700.

\[28\]Sekiguchi H, Watanabe M, Nakahara T, Uchiyama H. Succession of bacterial community structure along the Changjiang River determined by denaturing gradient gel electrophoresis and clone library analysis. Appl Environ Microbiol, 2002, 68(13): 51425150.

\[29\]倪学勤,   Gong J, Yu H, 等. PCRDGGE技术分析蛋鸡MHC基因对肠道细菌种群结构的影响. 中国农业科学, 2009, 42(7): 25642571.

\[30\]刘淑平, 王新, 徐红星, 等. 应用巢式PCRDGGE技术分析稻虱缨小蜂体内Wolbachia的多样性. 昆虫学报, 2011, 54(12): 13541360.

\[31\]Otuka A, Matsumura M, Watanabe T, et al. A migration analysis for rice planthoppers, Sogatella furcifera (Horváth) and Nilaparvata lugens (Stl) (Delphacidae), emigrating from northern Vietnam from April to May. Appl Entomol Zool, 2008, 43(4): 527534.

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褐飞虱体内细菌群落的PCRDGGE分析

PCRDGGE Analysis of the Bacterial Community in Different Populations of Rice Brown Planthopper, Nilaparvata lugens Stl

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