水稻新种质YSBR1抗纹枯病机制的初步研究

doi:10.3969/j.issn.1001-7216.2014.02.004

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (2): 132-140.DOI: 10.3969/j.issn.1001-7216.2014.02.004

水稻新种质YSBR1抗纹枯病机制的初步研究

左示敏^*,张玉梅,薛芗,朱亚军,张亚芳,陈宗祥,陈夕军,潘学彪^*

扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室，江苏扬州 225009；

收稿日期:2013-10-03 修回日期:2013-11-08 出版日期:2014-03-10 发布日期:2014-03-10
通讯作者: 左示敏* ,潘学彪*
基金资助:
国家转基因生物新品种培育重大专项（2012ZX08009001）；江苏省农业科技自主创新资金资助项目\[CX（12）1003\]；江苏省科技支撑计划资助项目（BE2012303）；江苏省高校自然科学基金资助项目（12KJB210007）；扬州大学科技创新基金资助项目（2012CXJ060）。

Preliminary Study on Resistance Mechanism of the New Rice Line YSBR1 to Sheath Blight

ZUO Shimin^* , ZHANG Yumei, XUE Xiang, ZHU Yajun, ZHANG Yafang, CHEN Zongxiang, CHEN Xijun, PAN Xuebiao ^*

Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Agriculture, Yangzhou University/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou 225009, China;

Received:2013-10-03 Revised:2013-11-08 Online:2014-03-10 Published:2014-03-10
Contact: ZUO Shimin*,PAN Xuebiao*

摘要/Abstract

摘要： 通过测定蜡质含量、硅化细胞、硅含量以及相关防御酶和防卫基因诱导表达特征，对抗纹枯病品系YSBR1的抗性机制进行了初步研究。结果显示，叶片和叶鞘的蜡质含量在不同抗、感品种间差异不显著；叶片上、下表面的硅化细胞排列密度在各品种间总体差异不明显，各品种叶鞘内侧均未发现硅化细胞；纹枯菌接种后YSBR1叶片下表面和叶鞘内表面硅元素含量均显著或极显著上升，中抗品种Jasmine 85叶鞘内表面硅含量亦极显著上升，感病品种Lemont不同部位的硅含量在处理之间差异均不显著；纹枯病菌接种后YSBR1中的苯丙氨酸解氨酶和多酚氧化酶活性的诱导表达及活性高峰值总体上快于或高于其它供试品种；接种后12 h，防卫基因OsPR4的表达量在YSBR1中急速上升且明显高于Jasmine 85和Lemont，OsPR10在特青中为诱导表达，但在YSBR1和Lemont中为组成型强表达。综上，YSBR1对纹枯病的高水平抗性与其防卫反应的快速启动有关。

关键词: 水稻纹枯病, 抗病种质, 蜡质, 硅, 防御反应

Abstract: The preliminary mechanism of the new rice line YSBR1 with high resistance to sheath blight disease(SB) was investigated through analyzing the content of wax and silicon, the arrangement of silicon cell, the activity of the wellknown defense enzymes, and the expression pattern of some pathogenesisrelated (PR) genes in response to SB fungus infection. The wax contents of leaves and leaf sheaths were not significantly different among varieties with various resistance levels to SB disease including YSBR1. The arrangement of silicon cells in the upper and lower surfaces of leaf blades was similar among varieties, and no silicon cell was found in the inner surfaces of leaf sheaths. In response to SB fungus infection, the contents of silicon in the lower surfaces of leaf blades and the inner surfaces of leaf sheaths in YSBR1 were significantly induced, as well as in inner surfaces of leaf sheaths of Jasmine 85 with moderate resistance to SB. However, it was not found in all tissues tested in susceptible Lemont. In YSBR1, the activities of phenylalanine ammonia lyase(PAL ) and polyphenol oxidase(PPO) were induced faster and significantly higher by SB fungus than those in other varieties. The expression level of OsPR4 in YSBR1 was upregulated rapidly and obviously higher than those in Jasmine 85 and Lemont 12 h post inoculation. The OsPR10 gene was found constitutively expressed in YSBR1 and Lemont, but induced expression in Jasmine 85 by SB fungus infection. Taken together, our results suggest that the defense system of YSBR1 is rapidly activated upon SB fungus infection,which is responsible for its high resistance to SB.

Key words: rice sheath blight, resistant variety, wax content, silicon, defense response

中图分类号:

左示敏* ,张玉梅,薛芗,朱亚军,张亚芳,陈宗祥,陈夕军,潘学彪*. 水稻新种质YSBR1抗纹枯病机制的初步研究[J]. 中国水稻科学, 2014, 28(2): 132-140.

ZUO Shimin*, ZHANG Yumei, XUE Xiang, ZHU Yajun, ZHANG Yafang, CHEN Zongxiang, CHEN Xijun, PAN Xuebiao*. Preliminary Study on Resistance Mechanism of the New Rice Line YSBR1 to Sheath Blight[J]. Chinese Journal of Rice Science, 2014, 28(2): 132-140.

参考文献

\[1\]廖皓年, 肖陵生, 王华生, 等. 水稻纹枯病发生历史及演变原因简析. 广西植保, 1997（3）: 3538.

\[2\]Lee F N, Rush M C. Rice sheath blight: A major rice disease. Plant Dis, 1983, 67:829832.

\[3\]Zhen A P, Lin R M, Zhang D H, et al. The evolution and pathogenic mechanisms of the rice sheath blight pathogen. Nat Commun, 2013, 4: 1424.

\[4\]左示敏, 张亚芳, 陈宗祥, 等. 水稻抗纹枯病遗传育种研究进展. 中国科学: 生命科学, 2010, 40(11): 10141023.

\[5\]Liu G, Jia Y, McClung A, et al. Confirming QTLs and finding additional loci responsible for resistance to rice sheath blight disease. Plant Dis, 2013, 97(1): 113117.

\[6\]Zuo S M, Yin Y J, Pan C H, et al. Fine mapping of qSB11LE, the QTL that confers partial resistance to rice sheath blight. Theor Appl Genet, 2013, 126: 12571272.

\[7\]童蕴慧, 徐敬友, 潘学彪, 等. 水稻植株对纹枯病菌侵染反应及其机理的初步研究. 江苏农业研究, 2000, 21(4): 4547.

\[8\]陈志谊, 王玉环, 殷尚志. 水稻纹枯病抗性机制的研究. 中国农业科学, 1992, 25(4): 4146.

\[9\]张国良, 戴其根, 张洪程. 施硅增强水稻对纹枯病的抗性. 植物生理与分子生物学学报, 2006, 32(5): 600606.

\[10\]Datnoff L E, Deren C W, Snyder G H. Silicon fertilization for disease management of rice in Florida. Crop Prot, 1997(6):525531.

\[11\]Zhang G L, Cui Y X, Ding X W, et al. Stimulation of phenolic metabolism by silicon contributes to rice resistance to sheath blight. J Plant Nutr Soil Sci, 2013, 176:118124.

\[12\]吴样孙, 陈一壮, 蒙信满, 等. 水稻纹枯病抗性反应中主要防御酶的活性变化.中国农学通报, 2008(5): 327330.

\[13\]敖世恩, 杨媚, 周而勋, 等. 离体筛选的水稻抗纹枯病突变体的生理生化特性分析.华南农业大学学报, 2006, 27(2): 3941.

\[14\]赵长江, 鲁国东, 杜晓昱, 等. 水稻纹枯病发病过程PR1和PBZ1的表达动态. 植物病理学报. 2006, 36(4): 317321.

\[15\]左示敏, 王子斌, 陈夕军, 等. 水稻纹枯病改良新抗源YSBR1的抗性评价. 作物学报, 2009, 35(4): 608614.

\[16\]袁隆平. 新株型育种进展. 杂交水稻, 2011, 26(4): 7274.

\[17\]陈夕军, 王玲, 左示敏, 等. 水稻纹枯病寄主病原物互作鉴别品种与菌株的筛选. 植物病理学报, 2009, 39(5): 514520.

\[18\]王子斌, 左示敏, 李刚, 等. 水稻纹枯病几种田间病级调查标准的比较研究. 扬州大学学报: 农业与生命科学版, 2009, 30(4): 5762.

\[19\]王子斌, 李刚, 左示敏, 等. 一个F2 无性系群体的纹枯病抗性QTL的初步定位研究. 扬州大学学报: 农业与生命科学版, 2010, 31(4): 2830.

\[20\]周小云, 陈信波, 徐向丽, 等. 稻叶表皮蜡质提取方法及含量的比较.湖南农业大学学报: 自然科学版, 2007, 33(3): 273276.

\[21\]Wang N L, Xiao B Z, Xiong L Z. Identification of a cluster of PR4like genes involved in stress responses in rice. J Plant Physiol, 2011, 168(18): 22122224.

\[22\]乐美旺, 陈实, 潘庆华, 等. 水稻和稻瘟病菌互作中的信号传导及防御反应基因诱导表达的研究. 植物病理学报, 2007, 37(1): 4249.

\[23\]左示敏, 张亚芳, 殷跃军, 等. 田间水稻纹枯病抗性鉴定体系的确立与完善. 扬州大学学报: 农业与生命科学版, 2006, 27(4): 5761.

\[24\] Chen X B, Mark Goodwin S, Virginia L, et al．Cloning and characterization of the WAX2 gene of Arabidopsis involved in cuticle membrane and wax production. Plant Cell, 2003, 15: 11701185.

\[25\]Jetter R, Schaffer S. Dynamic change of the epicuticular wax film during leaf development. Plant Physiol, 2001, 126:17251737.

\[26\]Mariani C, Wolters M. Complex waxes. Plant Cell, 2000, 12: 17951798.

\[27\]冯东昕, 李宝栋. 可溶性硅在植物抵御病害中的作用.植物病理学报, 1998, 28(4): 293297.

\[28\]Zhu T, Song F, Zheng Z. Molecular characterization of the rice pathogenesisrelated protein, OsPR4b, and its antifungal activity against Rhizoctonia solani. J Phytopathol, 2006, 154: 378384.

\[29\]Agrawal G K, Jwa N S, Han K S, et al. Isolation of a novel rice PR4 type gene whose mRNA expression is modulated by blast pathogen attack and signaling components. Plant Physiol Biochem, 2003, 41:8190.

\[30\]Glazebrook J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol, 2005, 43: 205227.

\[31\]Chern M S, Fitzgerald H A, Yadav R, et al. Evidence for a diseaseresistance pathway in rice similar to the NPR1mediated signaling pathway in Arabidopsis. Plant J, 2001, 27(2): 101113.

\[32\]左示敏, 殷跃军, 张亚芳, 等. 植物数量抗病基因克隆及其抗性机理的研究进展. 分子植物育种, 2006 (5): 603613.

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水稻新种质YSBR1抗纹枯病机制的初步研究

Preliminary Study on Resistance Mechanism of the New Rice Line YSBR1 to Sheath Blight

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