磷转运蛋白OsPT6在水稻武育粳7号苗期磷素吸收利用中的作用

doi:10.3969/j.issn.1001-7216.2014.01.001

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (1): 1-8.DOI: 10.3969/j.issn.1001-7216.2014.01.001

• 研究报告 • 下一篇

磷转运蛋白OsPT6在水稻武育粳7号苗期磷素吸收利用中的作用

张芳^#,艾昊^#,王丹凤,曹越,史书林,顾冕,孙淑斌^*,徐国华

南京农业大学资源与环境科学学院, 南京210095;

收稿日期:2013-02-03 修回日期:2013-07-04 出版日期:2014-01-10 发布日期:2014-01-10
通讯作者: 孙淑斌*
基金资助:
国家自然科学基金资助项目（31172014, 30871582）。

Roles of OsPT6 on the Phosphate Uptake and Utilization in the Rice Seeding of Wuyujing 7

ZHANG Fang^#, AI Hao^#, WANG Danfeng, CAO Yue, SHI Shulin, GU Mian, SUN Shubin^*, XU Guohua

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;

Received:2013-02-03 Revised:2013-07-04 Online:2014-01-10 Published:2014-01-10
Contact: SUN Shubin*,

摘要/Abstract

摘要： 磷转运蛋白OsPT6为水稻Pht1家族成员之一，具有吸收和转运磷酸盐双重功能。以水稻高产品种武育粳7号的OsPT6超表达转基因材料为试材，研究磷转运蛋白OsPT6在武育粳7号磷素吸收利用中的作用。结果表明：1)OsPT6在武育粳7号的地下部表达丰度较高，同时在地下部和地上部该基因均受缺磷诱导表达量显著增加。2)OsPT6超表达后，转基因植株生长良好。正常供磷和低磷处理35 d后,OsPT6超表达植株地上部和地下部干物质量都显著增加。3)OsPT6超表达转基因材料在不同浓度磷素处理后，各组织全磷含量有所增加，其中营养器官尤为显著。

关键词: 水稻, 磷转运蛋白, 超表达

Abstract: As one of members of the Pht1 transporters family in rice,the phosphate transporter OsPT6 plays a broad role in Pi uptake, translocation and internal transport throughout the plant. With the OsPT6 overexpressed transgenic rice of Wuyujing 7, a highyielding variety, we studied the roles of OsPT6 on the phosphate uptake and utilization in rice. The main results are the following: 1)OsPT6 is abundantly expressed in roots of Wuyujing 7. Furthermore, the expression levels of OsPT6 in shoots and roots are induced by phosphate deficiency. 2)The overexpression of OsPT6 can promote the growth of plants. The dry weight of shoots and roots of the OsPT6 overexpressed transgenic plants was significantly increased, in both sufficient and deficientPi conditions for 35 days. 3)The total P concentration increases in different tissues and organs of OsPT6 overexpressed transgenic plants, especially in vegetable organs, at different Pisupply levels.

Key words: rice, phosphate transporter, overexpression

中图分类号:

张芳#,艾昊#,王丹凤,曹越,史书林,顾冕,孙淑斌*,徐国华. 磷转运蛋白OsPT6在水稻武育粳7号苗期磷素吸收利用中的作用[J]. 中国水稻科学, 2014, 28(1): 1-8.

ZHANG Fang#, AI Hao#, WANG Danfeng, CAO Yue, SHI Shulin, GU Mian, SUN Shubin*, XU Guohua. Roles of OsPT6 on the Phosphate Uptake and Utilization in the Rice Seeding of Wuyujing 7[J]. Chinese Journal of Rice Science, 2014, 28(1): 1-8.

参考文献

\[1\]Theodorou M E, Plaxton W C. Metabolic adaptations of plant respiration to nutritional phosphate deprivation. Plant Physiol, 1993, 101: 339344.

\[2\]Mimura T. Regulation of phosphate transport and homeostasis in the plant cells. Int Rev Cytol, 1999, 191: 149200.

\[3\]Vance C P, UhdeStone C, Allan D L. Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytol, 2003, 157: 423447.

\[4\]Schachtman D P, Reid R J, Ayling S M. Phosphorus uptake by plants: From soil to cell. Plant Physiol, 1998, 116: 447453.

\[5\]Raghothama K G. Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol, 1999, 150: 665693.

\[6\]Smith F W, Mudge S R, Rae A L, et al. Phosphate transporter in plants. Plant Soil, 2003, 248: 7183.

\[7\]Muchhal U S, Pardo J M, Raghothama K G. Phosphate transporters from the higher plant Arabidopsis thaliana. Proc Natl Acad Sci USA, 1996, 93: 1051910523.

\[8\]Rausch C, Bucher M. Molecular mechanisms of phosphate transport in plants. Planta, 2002, 16: 2337.

\[9\]Mudge S R, Rae A L, Diatloff E, et al. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J, 2002, 31: 341351.

\[10\]Rae A L, Cybinski D H, Jarmey J M, et al. Characterization of two phosphate transporters from barley: Evidence for diverse function and kinetic properties among members of the Pht1 family. Plant Mol Biol, 2003, 53: 2736.

\[11\] Poirier Y, Bucher M. Phosphate transport and homeostasis in Arabidopsis//Somerville C R, Meyerowitz E M. The Arabidopsis Book. Rockville, M D: Am Soc Plant Biol, 2002: 24.

\[12\] Chen A Q, Hu J, Sun S B, et al. Conservation and divergence of both phosphate and mycorrhizaregulated physiological responses and expression patterns of phosphate transporters in solanaceous species. New Phytol, 2007, 173: 817831.

\[13\] Paszkowski U, Kroken S, Roux C, et al. Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis. Proc Natl Acad Sci USA, 2002, 99: 1332413329.

\[14\] Sun S B, Gu M, Cao Y, et al. A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphatereplete rice. Plant Physiol, 2012, 159: 15711581.

\[15\] Jia H F, Ren H Y, Gu M, et al. Phosphate transporter gene, OsPht1; 8, is involved in phosphate homeostasis in rice. Plant Physiol, 2011, 156: 11641175.

\[16\] Ai P H, Sun S B, Zhao J N, et al. Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation. Plant J， 2009, 57: 798809.

\[17\] 周群演, 蒋红梅. “武运粳7号”主要特性与高产栽培技术. 上海农业科技, 2000(3): 3133.

\[18\] 鲍士旦. 土壤农化分析. 北京：中国农业出版社， 1999.

\[19\] Elliott G C, Lauchli A. Phosphorus efficiency and phosphate iron interactions in maize. Agron J, 1985, 77: 399403.

\[20\] Siddiqi M Y, Glass A D. Utilization index: A modified approach to the estimation and comparison of nutrient utilization efficiency in plants. J Plant Nutr, 1981, 4: 289302.

\[21\] Shin H, Shin H S, Dewbre G R, et al. Phosphate transport in Arabidopsis: Pht1;1 and Pht1;4 play a major role in phosphate acquisition from both low and highphosphate environments. Plant J, 2004, 39: 629642.

\[22\] Chen A Q, Hu J, Sun S, et al. Conservation and divergence of both phosphate and mycorrhizaregulated physiological responses and expression patterns of phosphate transporters in solanaceous species. New Phytol, 2007, 173: 817831.

\[23\] Misson J, Thibaud M C, Bechtold N, et al. Transcriptional regulation and functional properties of Arabidopsis Pht1;4, a high affinity transporter contributing greatly to phosphate uptake in phosphate deprived plants. Plant Mol Biol, 2004, 55: 727741.

\[24\] Remy E, Cabrito T R, Batista1 R A, et al. The Pht1;9 and Pht1;8 transporters mediate inorganic phosphate acquisition by the Arabidopsis thaliana root during phosphorus starvation. New Phytol, 2012, 195: 356371.

\[25\] Mudge S R, Rae A L, Diatloff E, et al. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J, 2002, 31: 341353.

\[26\] Liu F, Wang Z, Ren H, et al. OsSPX1 suppresses the function of OsPHR2 in the regulation of expression of OsPT2 and phosphate homeostasis in shoots of rice. Plant J, 2010, 62: 508517.

[1]	郭展, 张运波. 水稻对干旱胁迫的生理生化响应及分子调控研究进展[J]. 中国水稻科学, 2024, 38(4): 335-349.
[2]	韦还和, 马唯一, 左博源, 汪璐璐, 朱旺, 耿孝宇, 张翔, 孟天瑶, 陈英龙, 高平磊, 许轲, 霍中洋, 戴其根. 盐、干旱及其复合胁迫对水稻产量和品质形成影响的研究进展[J]. 中国水稻科学, 2024, 38(4): 350-363.
[3]	许丹洁, 林巧霞, 李正康, 庄小倩, 凌宇, 赖美玲, 陈晓婷, 鲁国东. OsOPR10正调控水稻对稻瘟病和白叶枯病的抗性[J]. 中国水稻科学, 2024, 38(4): 364-374.
[4]	候小琴, 王莹, 余贝, 符卫蒙, 奉保华, 沈煜潮, 谢杭军, 王焕然, 许用强, 武志海, 王建军, 陶龙兴, 符冠富. 黄腐酸钾提高水稻秧苗耐盐性的作用途径分析[J]. 中国水稻科学, 2024, 38(4): 409-421.
[5]	胡继杰, 胡志华, 张均华, 曹小闯, 金千瑜, 章志远, 朱练峰. 根际饱和溶解氧对水稻分蘖期光合及生长特性的影响[J]. 中国水稻科学, 2024, 38(4): 437-446.
[6]	刘福祥, 甄浩洋, 彭焕, 郑刘春, 彭德良, 文艳华. 广东省水稻孢囊线虫病调查与鉴定[J]. 中国水稻科学, 2024, 38(4): 456-461.
[7]	陈浩田, 秦缘, 钟笑涵, 林晨语, 秦竞航, 杨建昌, 张伟杨. 水稻根系和土壤性状与稻田甲烷排放关系的研究进展[J]. 中国水稻科学, 2024, 38(3): 233-245.
[8]	缪军, 冉金晖, 徐梦彬, 卜柳冰, 王平, 梁国华, 周勇. 过量表达异三聚体G蛋白γ亚基基因RGG2提高水稻抗旱性[J]. 中国水稻科学, 2024, 38(3): 246-255.
[9]	尹潇潇, 张芷菡, 颜绣莲, 廖蓉, 杨思葭, 郭岱铭, 樊晶, 赵志学, 王文明. 多个稻曲病菌效应因子的信号肽验证和表达分析[J]. 中国水稻科学, 2024, 38(3): 256-265.
[10]	朱裕敬, 桂金鑫, 龚成云, 罗新阳, 石居斌, 张海清, 贺记外. 全基因组关联分析定位水稻分蘖角度QTL[J]. 中国水稻科学, 2024, 38(3): 266-276.
[11]	魏倩倩, 汪玉磊, 孔海民, 徐青山, 颜玉莲, 潘林, 迟春欣, 孔亚丽, 田文昊, 朱练峰, 曹小闯, 张均华, 朱春权. 信号分子硫化氢参与硫肥缓解铝对水稻生长抑制作用的机制[J]. 中国水稻科学, 2024, 38(3): 290-302.
[12]	周甜, 吴少华, 康建宏, 吴宏亮, 杨生龙, 王星强, 李昱, 黄玉峰. 不同种植模式对水稻籽粒淀粉含量及淀粉关键酶活性的影响[J]. 中国水稻科学, 2024, 38(3): 303-315.
[13]	关雅琪, 鄂志国, 王磊, 申红芳. 影响中国水稻生产环节外包发展因素的实证研究：基于群体效应视角[J]. 中国水稻科学, 2024, 38(3): 324-334.
[14]	许用强, 姜宁, 奉保华, 肖晶晶, 陶龙兴, 符冠富. 水稻开花期高温热害响应机理及其调控技术研究进展[J]. 中国水稻科学, 2024, 38(2): 111-126.
[15]	吕海涛, 李建忠, 鲁艳辉, 徐红星, 郑许松, 吕仲贤. 稻田福寿螺的发生、危害及其防控技术研究进展[J]. 中国水稻科学, 2024, 38(2): 127-139.

磷转运蛋白OsPT6在水稻武育粳7号苗期磷素吸收利用中的作用

Roles of OsPT6 on the Phosphate Uptake and Utilization in the Rice Seeding of Wuyujing 7

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics